Archive BacDiveID:10.13145/bacdive18157.20230509.8.1

Strain identifier

BacDive ID: 18157

Type strain:

Species: Cystobacter sp.

NCBI tax ID(s): 1965334 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 18281

BacDive-ID: 18157

keywords: Bacteria, mesophilic, motile, rod-shaped, colony-forming

description: Cystobacter sp. Cb 502 is a mesophilic, motile, rod-shaped bacterium that forms bulky tough slimy dark reddish brown swarm with radial wires and cross folds, medium surround the swarm also brownish colored, no fruiting bodies on this medium colonies and builds a fruiting body with sporangioles and spores.

NCBI tax id

NCBI tax id: 1965334
Matching level: species

doi: 10.13145/bacdive18157.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Deltaproteobacteria
order: Myxococcales
family: Archangiaceae
genus: Cystobacter
species: Cystobacter sp.
full scientific name: Cystobacter Schroeter 1886 (Approved Lists 1980)

@ref: 18281

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Cystobacteraceae

genus: Cystobacter

species: Cystobacter sp.

full scientific name: Cystobacter sp.

type strain: no

Morphology

cell morphology

@ref: 18281
cell length: 4.7 µm
cell width: 0.8 µm
cell shape: rod-shaped
motility: yes

colony morphology

@ref	colony color	colony shape	medium used
18281	dark reddish brown	bulky tough slimy dark reddish brown swarm with radial wires and cross folds, medium surround the swarm also brownish colored, no fruiting bodies on this medium	cy-agar
18281		swarms structured by radial veining, containing rudimentarily developed fruiting bodies: Nests of lightly packed colorless, medium sized, translucent sporangioles, mostly embedded in agar.	VY/2 - Agar
18281		growth medium strong; comparable to growth on VY/2 agar translucent sporangioles are lightly brown colored	VY/5

multicellular morphology

@ref: 18281
forms multicellular complex: yes
complex name: fruiting body with sporangioles and spores
complex color: golden yellow
further description: Tough, easily peelable slimy coating. Sporangiole are spherical to oval; crowded, densely in clusters, golden yellow colored, small, bear a striking similarity to Sorangium. Myxospores are short, thick, often bean-shaped, non-refractile, combined with 2 or 1 refractile granules, 1.7-2.2 µm long (usually about 2 µm) and 0.95-1.4 µm thick (usually about 1 µm). Sporangioles with distinct rough shells. Swarms often in oscillating waves. Sizes may differ on distinct cultivation media.

multimedia

@ref	multimedia content	caption	intellectual property rights
18281	/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_nummerierte_Staemme/Cb_502/	Cb 502	Helmholtz-Zentrum für Infektionsforschung GmbH
18281	/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_nummerierte_Staemme/Cb_502/	Cb 502	Helmholtz-Zentrum für Infektionsforschung GmbH
18281	/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_nummerierte_Staemme/Cb_502/	Cb 502	Helmholtz-Zentrum für Infektionsforschung GmbH
18281	/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_nummerierte_Staemme/Cb_502/	Cb 502	Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@ref	name	composition	growth
18281	VY/2 AGAR	Baker's yeast 5.00 g CaCl₂ x 2 H₂O 1.36 g Vitamin B₁₂ 0.50 mg Agar (Difco) 15.00 g Distilled water 1000.00 ml Sterilize vitamin B₁₂ separately by filtration. Prepare and store yeast cells as autoclaved stocksuspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50°C (use pH-indicator paper).
18281	VY/5 - Agar	Baker's yeast 0.2% CaCl₂ x 2 H₂O 0.1% MgSO₄ x 7 H₂O 0.15% Agar (Difco) 1,5% Vitamin B₁₂ 0.50 g/ml Agar (Difco) 1,5% Vitamin B₇(Biotin) 0.50 g/ml Distilled water 1000.00 ml Sterilize vitamins separately by filtration. Prepare and store yeast cells as autoclaved stocksuspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50°C (use pH-indicator paper).
18281	VY/2 - Agar	Baker's yeast 5.00 g CaCl₂ x 2 H₂O 1.36 g Vitamin B₁₂ 0.50 mg Agar (Difco) 15.00 g Distilled water 1000.00 ml Sterilize vitamin B₁₂ separately by filtration. Prepare and store yeast cells as autoclaved stocksuspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50°C (use pH-indicator paper).
18281	VY/5	Baker's yeast 0.2% CaCl₂ x 2 H₂O 0.1% MgSO₄ x 7 H₂O 0.15% Agar (Difco) 1,5% Vitamin B₁₂ 0.50 g/ml Agar (Difco) 1,5% Vitamin B₇(Biotin) 0.50 g/ml Distilled water 1000.00 ml Sterilize vitamins separately by filtration. Prepare and store yeast cells as autoclaved stocksuspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50°C (use pH-indicator paper).
18281	CHITIN 3 AGAR	Ingredients: Yeast extract (Difco) 0.05% MgSO4 x 7H2O 0.1% Agar 1.2% Preparation / Remarks: pH 7.2 After autoclaving add 10 mL of sterile Chitin suspension (100 g/L Chitin in H2O) to 100 mL of the medium and pour on cy-plates.	yes
18281	CHITIN 4 AGAR	Ingredients: Yeast extract (Difco) 0.05% MgSO4 x 7H2O 0.1% Agar 1.2% Preparation / Remarks: pH 7.2 After autoclaving add 10 mL of sterile Chitin suspension (100 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates.	no
18281	CY-AGAR	Ingredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 (KOH) autoclaved	yes
18281	PROBION L AGAR	Ingredients: Probion L 600 (Hoechst) 0.8% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 autoclaved	yes
18281	VY/2 AGAR	Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved	yes
18281	VY/5 AGAR	Ingredients: Baker's yeast 0.2% (based on fresh weight) CaCl2 x 2H2O 0.1% MgSO4 x 7H2O 0.15% Vitamin B12 0.05 % Biotin 0.5 g/mL Agar 1.5% Preparation / Remarks: pH 7.2 (KOH) autoclaved	yes

culture temp

@ref: 18281
range: mesophilic

culture pH

@ref: 18281
ability: positive
type: optimum
pH: 7.2

Physiology and metabolism

observation

@ref	observation
18281	Strong ability to Yeast lysis
18281	Ability of bacterial lysis medium
18281	No ability for chitin degradation
18281	Ability for Yeast lysis

Isolation, sampling and environmental information

isolation

@ref: 18281
sample type: compost material
sampling date: 1974-06-01
geographic location: Freiburg/Breisgau
country: Germany
origin.country: DEU
continent: Europe
latitude: 48.0101
longitude: 7.85608
enrichment culture: w+Sarc (water agar + nutrient bacteria cells)
enrichment culture composition: Agar (Difco) 15.0g CaCl2 x 2 H2O 0.1% Distilled water 1000.0 ml Adjust pH of agar to 7.2 with KOH before autoclaving. Freshly grown Micrococcus luteus cells serve as food bacteria for the myxobacteria. The M. luteus cells are harvested from complex medium such as nutrient agar or broth, and mixed with sterile water to yield a paste. For example, a pellet after centrifugation is mixed with about the same volume of sterile water. Streak lines or a cross of the bacterial paste onto the agar s
enrichment culture duration: 21 days
enrichment culture temperature: 20

isolation source categories

Cat1: #Engineered
Cat2: #Biodegradation
Cat3: #Composting

External links

@ref: 18281

culture collection no.: Cb 502

Reference

@id	authors	title	doi/url
18281	Reichenbach, H.	Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ	10.1099/ijsem.0.004332