Strain identifier

BacDive ID: 18074

Type strain: Yes

Species: Blautia luti

Strain Designation: BInIX

Strain history: DSM 14534 <-- D. Taras <-- G. Le Blay.

NCBI tax ID(s): 649762 (strain), 89014 (species)

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General

@ref: 5286

BacDive-ID: 18074

DSM-Number: 14534

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive

description: Blautia luti BInIX is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from Human feces.

NCBI tax id

NCBI tax idMatching level
89014species
649762strain

strain history

@refhistory
5286<- D.Taras <- G. Le Blay, BInIX
67770DSM 14534 <-- D. Taras <-- G. Le Blay.

doi: 10.13145/bacdive18074.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Blautia
  • species: Blautia luti
  • full scientific name: Blautia luti (Simmering et al. 2002) Liu et al. 2008

  • synonyms

    • @ref: 20215
    • synonym: Ruminococcus luti

@ref: 5286

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Blautia

species: Blautia luti

full scientific name: Blautia luti (Simmering et al. 2002) Liu et al. 2008

strain designation: BInIX

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 99.719

Culture and growth conditions

culture medium

  • @ref: 5286
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
5286positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
5286anaerobe
56852anaerobe
69480anaerobe99.998

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.366

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea+hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase+3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase+3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380urease+3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
5286+-++-+++-----+---------------
5286++++-+++-----+---------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling date
5286Human fecesGermanyDEUEurope
56852Human fecesGermanyDEUEurope1999-02-01
67770Human feces

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

  • @ref: 5286
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Blautia luti gene for 16S rRNA, partial sequence, strain: DSM 14534AB6915761481ena649762
5286Blautia luti 16S rRNA gene, strain bln9AJ1331241335ena89014
67770Blautia luti gene for 16S ribosomal RNA, partial sequence, strain: JCM 17040LC0106921492ena89014

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Blautia luti DSM 14534649762.4wgspatric649762
67770Blautia luti DSM 14534 = JCM 17040GCA_009707925contigncbi649762

GC content

@refGC-contentmethod
528643.3
6777043.3high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno68no
gram-positiveyes87.676no
anaerobicyes99.146no
halophileno79.632no
spore-formingno65.467no
glucose-utilyes89.189no
thermophileno98.97no
flagellatedno91.816no
aerobicno98.425yes
motileno86.887no
glucose-fermentyes80.66no

External links

@ref: 5286

culture collection no.: DSM 14534, CCUG 45635, JCM 17040, KCTC 5974, CIP 107222#, JCM 14658#, CIP 107222, KCTC 15704

straininfo link

  • @ref: 87062
  • straininfo: 100937

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12353871Ruminococcus luti sp. nov., isolated from a human faecal sample.Simmering R, Taras D, Schwiertz A, Le Blay G, Gruhl B, Lawson PA, Collins MD, Blaut MSyst Appl Microbiol10.1078/0723-2020-001122002Bacteria/growth & development/isolation & purification/metabolism, Bacteria, Anaerobic/metabolism, Base Sequence, Feces/*microbiology, Genes, Bacterial, Gram-Positive Bacteria/classification/cytology/*isolation & purification, Humans, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/analysis, Sequence AlignmentEnzymology
Pathogenicity30451443Exopolysaccharides Produced by Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos Affect Their Adhesion to Enterocytes.Konieczna C, Slodzinski M, Schmidt MTPol J Microbiol10.21307/pjm-2018-0322018*Bacterial Adhesion, Caco-2 Cells, Enterocytes/*microbiology, Faecalibacterium/enzymology, Gastrointestinal Microbiome, Humans, Hydrolysis, Lactobacillus casei/*chemistry/physiology, Lactobacillus rhamnosus/*chemistry/physiology, Polysaccharides, Bacterial/*metabolism, ProbioticsEnzymology
Genetics32381603Draft Genome Sequence of Blautia luti DSM 14534(T), Isolated from Human Stool.Ortiz RL, Melis-Arcos F, Covarrubias PC, Ugalde JA, Apte ZS, Perez-Donoso J, Cardenas JP, Almonacid DEMicrobiol Resour Announc10.1128/MRA.00088-202020Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5286Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14534)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14534
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
56852Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 45635)https://www.ccug.se/strain?id=45635
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
87062Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100937.1StrainInfo: A central database for resolving microbial strain identifiers