Strain identifier

BacDive ID: 18024

Type strain: Yes

Species: Caldicellulosiruptor acetigenus

Strain Designation: 6A

Strain history: <- B. Ahring, 6 A

NCBI tax ID(s): 301953 (species)

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General

@ref: 3634

BacDive-ID: 18024

DSM-Number: 9545

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, chemoorganoheterotroph, thermophilic, Gram-negative, rod-shaped, colony-forming

description: Caldicellulosiruptor acetigenus 6A is an anaerobe, chemoorganoheterotroph, thermophilic bacterium that forms circular colonies and was isolated from alkaline hot spring.

NCBI tax id

  • NCBI tax id: 301953
  • Matching level: species

strain history

  • @ref: 3634
  • history: <- B. Ahring, 6 A

doi: 10.13145/bacdive18024.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Syntrophomonadaceae
  • genus: Caldicellulosiruptor
  • species: Caldicellulosiruptor acetigenus
  • full scientific name: Caldicellulosiruptor acetigenus (Nielsen et al. 1994) Onyenwoke et al. 2006

  • synonyms

    @refsynonym
    20215Caldicellulosiruptor kristjanssonii
    20215Caldicellulosiruptor lactoaceticus
    20215Thermoanaerobium acetigenum

@ref: 3634

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridia, not assigned to family

genus: Caldicellulosiruptor

species: Caldicellulosiruptor lactoaceticus

full scientific name: Caldicellulosiruptor lactoaceticus Mladenovska et al. 1997

strain designation: 6A

type strain: yes

Morphology

cell morphology

  • @ref: 43351
  • gram stain: negative
  • cell length: 1.5-3.5 µm
  • cell width: 0.7 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43351
  • colony size: 1 mm
  • colony color: white opalascent
  • colony shape: circular
  • incubation period: 7 days
  • medium used: avicel agar roll tube

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3634THERMOANAEROBACTER (BA) MEDIUM (DSMZ Medium 671)yeshttps://mediadive.dsmz.de/medium/671Name: THERMOANAEROBACTER (BA) MEDIUM (DSMZ Medium 671) Composition: Cellobiose 3.95648 g/l Cellulose 1.97824 g/l (optional) Na2CO3 0.98912 g/l NH4Cl 0.98912 g/l Yeast extract 0.74184 g/l K2HPO4 x 3 H2O 0.395648 g/l Na2S x 9 H2O 0.24728 g/l MgCl2 x 6 H2O 0.098912 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Nicotinic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water
43351avicel agar roll tubeyes

culture temp

  • @ref: 3634
  • growth: positive
  • type: growth
  • temperature: 70
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 3634
  • oxygen tolerance: anaerobe

nutrition type

  • @ref: 43351
  • type: chemoorganoheterotroph

spore formation

  • @ref: 43351
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43351NaClpositivegrowth0-0.5 %
43351NaClnogrowth1 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4335130089acetate-growth
4335122599arabinose-growth
4335118305arbutin-growth
43351casein-growth
4335117057cellobiose+growth
4335162968cellulose+degradation
4335116988D-ribose-growth
4335152071dextran-growth
4335116236ethanol-growth
4335128757fructose-growth
4335128260galactose-growth
4335117234glucose-growth
4335124996lactate-growth
4335117716lactose+growth
4335117306maltose+growth
4335129864mannitol-growth
4335137684mannose-growth
4335117790methanol-growth
4335117632nitrate-assimilation
4335117309pectin+degradation
4335115361pyruvate-growth
4335116634raffinose-growth
4335126546rhamnose-growth
4335130911sorbitol-growth
4335128017starch+degradation
4335117992sucrose-growth
4335127082trehalose-growth
4335137166xylan+degradation
4335118222xylose+growth
43351yeast extract (0.01 %, w/v)-growth

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
4335128971ampicillinyesyes10 µg/mL
4335117698chloramphenicolyesyes10 µg/mL
433516104kanamycinyesyes10 µg/mL
433517507neomycinyesyes10 µg/mL
4335117334penicillinyesyes10 µg/mL
4335117076streptomycinyesyes10 µg/mL
4335127902tetracyclineyesyes10 µg/mL
4335128001vancomycinyesyes10 µg/mL

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3634alkaline hot springHveragerdiIcelandISLEurope
43351alkaline hot spring in HveragerdiIcelandIcelandISLEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)
#Condition#Alkaline

taxonmaps

  • @ref: 69479
  • File name: preview.99_3934.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_153;96_509;97_797;98_2035;99_3934&stattab=map
  • Last taxonomy: Caldicellulosiruptor
  • 16S sequence: X82842
  • Sequence Identity:
  • Total samples: 24
  • soil counts: 2
  • aquatic counts: 15
  • animal counts: 7

Safety information

risk assessment

  • @ref: 3634
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 3634
  • description: Caldicellulosiruptor lactoaceticus partial 16S rRNA gene, type strain 6AT
  • accession: X82842
  • length: 1505
  • database: ena
  • NCBI tax ID: 632516

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Caldicellulosiruptor lactoaceticus 6A, DSM 95452506485007completeimg632516
66792Caldicellulosiruptor acetigenus 6AGCA_000193435completencbi632516

GC content

@refGC-contentmethod
363435.2high performance liquid chromatography (HPLC)
4335134.4-36high performance liquid chromatography (HPLC)

External links

@ref: 3634

culture collection no.: DSM 9545

straininfo link

  • @ref: 87013
  • straininfo: 48645

Reference

@idauthorscataloguedoi/urltitlejournal
3634Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9545)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9545
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
43351Zuzana Mladenovska, Indra M. Mathrani, Birgitte K. Ahring10.1007/BF00305357Isolation and characterization of Caldicellulosiruptor lactoaceticus sp. nov., an extremely thermophilic, cellulolytic, anaerobic bacteriumArch Microbiol 163: 223-230 1995
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87013Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48645.1StrainInfo: A central database for resolving microbial strain identifiers