Strain identifier
BacDive ID: 18018
Type strain: 
Species: Tissierella praeacuta
Strain Designation: BL 4858
Strain history: <- ATCC <- W.E.C. Moore, VPI 12193 <- California State Hlth Dept. (Strain BL 4858, Botulism Lab., CDC, Atlanta, Ga., USA).
NCBI tax ID(s): 43131 (species)
version 8.1 (current version)
General
@ref: 2275
BacDive-ID: 18018
DSM-Number: 5675
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, motile, human pathogen
description: Tissierella praeacuta BL 4858 is an anaerobe, spore-forming, mesophilic human pathogen that was isolated from abdominal abscess.
NCBI tax id
- NCBI tax id: 43131
- Matching level: species
strain history
- @ref: 2275
- history: <- ATCC <- W.E.C. Moore, VPI 12193 <- California State Hlth Dept. (Strain BL 4858, Botulism Lab., CDC, Atlanta, Ga., USA).
doi: 10.13145/bacdive18018.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Tissierellales
- family: Tissierellaceae
- genus: Tissierella
- species: Tissierella praeacuta
- full scientific name: Tissierella praeacuta (Tissier 1908) Collins and Shah 1986
synonyms
@ref synonym 20215 Clostridium hastiforme 20215 Bacteroides praeacutus 20215 Coccobacillus praeacutus
@ref: 2275
domain: Bacteria
phylum: Firmicutes
class: Tissierellia
order: Clostridiales
family: Tissierellaceae
genus: Tissierella
species: Tissierella praeacuta
full scientific name: Tissierella praeacuta (Tissier 1908) Collins and Shah 1986 emend. Alauzet et al. 2014
strain designation: BL 4858
type strain: no
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 91.962 | |
| 69480 | 100 | positive |
Culture and growth conditions
culture medium
- @ref: 2275
- name: CHOPPED MEAT MEDIUM (DSMZ Medium 78)
- growth: yes
- link: https://mediadive.dsmz.de/medium/78
- composition: Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 2275 | positive | growth | 37 | mesophilic |
| 47503 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 2275 | anaerobe | |
| 47503 | anaerobe | |
| 69480 | anaerobe | 99.999 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 2275 | abdominal abscess | ||||
| 47503 | Human abdominal abscess | California | USA | USA | North America |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host Body-Site | #Other | #Abdomen |
| #Host Body-Site | #Other | #Abscess |
taxonmaps
- @ref: 69479
- File name: preview.99_7347.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_207;96_3436;97_4218;98_5390;99_7347&stattab=map
- Last taxonomy: Tissierella praeacuta subclade
- 16S sequence: X80841
- Sequence Identity:
- Total samples: 2197
- soil counts: 286
- aquatic counts: 370
- animal counts: 1412
- plant counts: 129
Safety information
risk assessment
- @ref: 2275
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Tissierella praeacuta strain ATCC 33268 16S ribosomal RNA gene, partial sequence | DQ911276 | 319 | ena | 43131 |
| 20218 | Tissierella praeacuta strain ATCC 33268 16S ribosomal RNA gene, partial sequence | GQ461814 | 1415 | ena | 43131 |
| 20218 | Clostridium hastiforme 16S rRNA gene, strain DSM 5675 | X77848 | 1423 | ena | 43131 |
| 20218 | Clostridium hastiforme rrn gene for 16S ribosomal RNA, strain DSM 5675 | X80841 | 1521 | ena | 43131 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Tissierella praeacuta DSM 5675 | GCA_004346445 | contig | ncbi | 43131 |
| 66792 | Tissierella praeacuta strain DSM 5675 | 43131.5 | wgs | patric | 43131 |
| 66792 | Tissierella praeacuta DSM 5675 | 2802428849 | draft | img | 43131 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| gram-positive | yes | 77.814 | no |
| anaerobic | yes | 98.512 | yes |
| halophile | no | 83.638 | no |
| spore-forming | yes | 89.63 | no |
| glucose-util | yes | 76.089 | no |
| aerobic | no | 96.794 | no |
| flagellated | no | 82.795 | no |
| thermophile | no | 94.179 | yes |
| motile | yes | 82.83 | no |
| glucose-ferment | no | 58.92 | no |
External links
@ref: 2275
culture collection no.: CCUG 21837, KCTC 5022, NRRL B-41124, BCRC 14555, DSM 5675, ATCC 33268, VPI 12193
straininfo link
- @ref: 87007
- straininfo: 44945
literature
- topic: Phylogeny
- Pubmed-ID: 15143047
- title: Clostridium hastiforme is a later synonym of Tissierella praeacuta.
- authors: Bae JW, Park JR, Chang YH, Rhee SK, Kim BC, Park YH
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.63068-0
- year: 2004
- mesh: Clostridium/*classification/genetics/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Nucleic Acid Hybridization, Phenotype, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as Topic
- topic2: Phenotype
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 2275 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5675) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5675 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 47503 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 21837) | https://www.ccug.se/strain?id=21837 | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 87007 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID44945.1 | StrainInfo: A central database for resolving microbial strain identifiers |