Archive BacDiveID:10.13145/bacdive18015.20230509.8.1

Strain identifier

BacDive ID: 18015

Type strain:

Species: Tissierella creatinophila

Strain history: <- J. R. Andreesen <- A. Schleicher; KRE 4

NCBI tax ID(s): 1123403 (strain), 79681 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 2885

BacDive-ID: 18015

DSM-Number: 6911

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive

description: Tissierella creatinophila DSM 6911 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from anaerobic sewage sludge.

NCBI tax id

NCBI tax id	Matching level
79681	species
1123403	strain

strain history

@ref: 2885
history: <- J. R. Andreesen <- A. Schleicher; KRE 4

doi: 10.13145/bacdive18015.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/bacillota
domain: Bacteria
phylum: Bacillota
class: Clostridia
order: Tissierellales
family: Tissierellaceae
genus: Tissierella
species: Tissierella creatinophila
full scientific name: Tissierella creatinophila Harms et al. 1998

@ref: 2885

domain: Bacteria

phylum: Firmicutes

class: Tissierellia

order: Clostridiales

family: Tissierellaceae

genus: Tissierella

species: Tissierella creatinophila

full scientific name: Tissierella creatinophila Harms et al. 1998

type strain: yes

Morphology

cell morphology

@ref: 69480
gram stain: positive
confidence: 100

Culture and growth conditions

culture medium

@ref: 2885
name: TISSIERELLA CREATINOPHILA MEDIUM (DSMZ Medium 824)
growth: yes
link: https://mediadive.dsmz.de/medium/824
composition: Name: TISSIERELLA CREATINOPHILA MEDIUM (DSMZ Medium 824) Composition: Creatine 3.78486 g/l Na-formate 2.70916 g/l Yeast extract 1.99203 g/l Na2CO3 1.49402 g/l KCl 0.996016 g/l Na2S x 9 H2O 0.498008 g/l MgSO4 x 7 H2O 0.498008 g/l NaCl 0.498008 g/l NH4Cl 0.249004 g/l KH2PO4 0.199203 g/l CaCl2 x 2 H2O 0.149402 g/l HCl 0.00249004 g/l FeCl2 x 4 H2O 0.00149402 g/l NaOH 0.000498008 g/l Sodium resazurin 0.000498008 g/l Pyridoxine hydrochloride 0.000298805 g/l Thiamine-HCl x 2 H2O 0.000199203 g/l Nicotinic acid 0.000199203 g/l CoCl2 x 6 H2O 0.000189243 g/l Calcium pantothenate 9.96016e-05 g/l Vitamin B12 9.96016e-05 g/l MnCl2 x 4 H2O 9.96016e-05 g/l p-Aminobenzoic acid 7.96813e-05 g/l ZnCl2 6.97211e-05 g/l Riboflavin 4.98008e-05 g/l Thiamine HCl 4.98008e-05 g/l Calcium D-(+)-pantothenate 4.98008e-05 g/l (DL)-alpha-Lipoic acid 4.98008e-05 g/l Na2MoO4 x 2 H2O 3.58566e-05 g/l NiCl2 x 6 H2O 2.39044e-05 g/l D-(+)-biotin 1.99203e-05 g/l Folic acid 1.99203e-05 g/l Biotin 1.99203e-05 g/l H3BO3 5.9761e-06 g/l Na2WO4 x 2 H2O 3.98406e-06 g/l Na2SeO3 x 5 H2O 2.98805e-06 g/l CuCl2 x 2 H2O 1.99203e-06 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
2885	positive	growth	30	mesophilic
60579	positive	growth	30-34	mesophilic

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance	confidence
2885	anaerobe
60579	obligate anaerobe
69480	anaerobe	97.739

spore formation

@ref	spore formation	confidence
69481	yes	98
69480	yes	98.789

Isolation, sampling and environmental information

isolation

@ref	sample type	country	origin.country	continent
2885	anaerobic sewage sludge	Germany	DEU	Europe
60579	Sewage sludge,anaerobic	Germany	DEU	Europe

isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Sewage sludge
#Condition	#Anoxic (anaerobic)

taxonmaps

@ref: 69479
File name: preview.99_3584.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_207;96_1862;97_2238;98_2746;99_3584&stattab=map
Last taxonomy: Tissierella creatinophila subclade
16S sequence: GQ461823
Sequence Identity:
Total samples: 3691
soil counts: 427
aquatic counts: 996
animal counts: 2115
plant counts: 153

Safety information

risk assessment

@ref: 2885
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Tissierella creatinophila strain DSM 6911 16S ribosomal RNA gene, partial sequence	GQ461823	1400	ena	79681
2885	A.sobria cepS gene	X80277	1149	ena	646

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Tissierella creatinophila DSM 6911	GCA_001940565	contig	ncbi	1123403
66792	Tissierella creatinophila DSM 6911	1123403.4	wgs	patric	1123403

GC content

@ref: 2885
GC-content: 30
method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	yes	98	no
motile	yes	79.899	no
flagellated	no	72.569	no
gram-positive	yes	85.309	no
anaerobic	yes	97.767	yes
aerobic	no	97.642	no
halophile	no	66.736	no
spore-forming	yes	76.513	no
thermophile	no	89.395	no
glucose-util	yes	81.623	no
glucose-ferment	yes	61.742	no

External links

@ref: 2885

culture collection no.: DSM 6911, CCUG 55419, KRE 4

straininfo link

@ref	straininfo
87002	48324
87003	409024

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	9734055	Tissierella creatinophila sp. nov., a gram-positive, anaerobic, non-spore-forming, creatinine-fermenting organism.	Harms C, Schleicher A, Collins MD, Andreesen JR	Int J Syst Bacteriol	10.1099/00207713-48-3-983	1998	Base Sequence, Creatinine/metabolism, Fermentation, Gram-Positive Asporogenous Rods/*classification/growth & development/metabolism, Molecular Sequence Data	Metabolism
Genetics	28336595	Genome Sequence of Creatinine-Fermenting Tissierella creatinophila Strain KRE 4(T) (DSM 6911).	Nacke H, Daniel R, Poehlein A	Genome Announc	10.1128/genomeA.00051-17	2017

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
2885	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6911)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-6911
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
60579	Curators of the CCUG	Culture Collection University of Gothenburg (CCUG) (CCUG 55419)	https://www.ccug.se/strain?id=55419
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg		10.1093/nar/gkaa1025	BRENDA, the ELIXIR core data resource in 2021: new developments and updates	Nucleic Acids Res. 49: D498-D508 2020	33211880
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch		10.21203/rs.3.rs-2527258/v1	Predictions based on the model GenomeNet Sporulation v. 1
87002	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID48324.1	StrainInfo: A central database for resolving microbial strain identifiers
87003	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID409024.1	StrainInfo: A central database for resolving microbial strain identifiers