Strain identifier

BacDive ID: 18005

Type strain: Yes

Species: Peptoniphilus methioninivorax

Strain history: <- DSM <- AP Rooney, NRRL, NRRL B-23883

NCBI tax ID(s): 907224 (species)

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General

@ref: 16269

BacDive-ID: 18005

DSM-Number: 22461

keywords: 16S sequence, Bacteria, anaerobe, coccus-shaped

description: Peptoniphilus methioninivorax DSM 22461 is an anaerobe, coccus-shaped bacterium that was isolated from retail ground beef.

NCBI tax id

  • NCBI tax id: 907224
  • Matching level: species

strain history

@refhistory
16269<- A. P. Rooney, NRRL; NRRL-B23883
67771<- DSM <- AP Rooney, NRRL, NRRL B-23883

doi: 10.13145/bacdive18005.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Peptoniphilaceae
  • genus: Peptoniphilus
  • species: Peptoniphilus methioninivorax
  • full scientific name: Peptoniphilus methioninivorax Rooney et al. 2011

@ref: 16269

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Peptoniphilaceae

genus: Peptoniphilus

species: Peptoniphilus methioninivorax

full scientific name: Peptoniphilus methioninivorax Rooney et al. 2011

type strain: yes

Morphology

cell morphology

@refcell shapegram stain
67771coccus-shaped
67771positive

Culture and growth conditions

culture medium

  • @ref: 16269
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperature
16269positivegrowth37
67771positivegrowth37

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16269anaerobe
67771anaerobe

spore formation

  • @ref: 67771
  • spore formation: no

murein

  • @ref: 16269
  • murein short key: A21.05
  • type: A4ß L-Orn-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
16269-+--------------+++--+-++++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16269retail ground beefFarmington, ILUSAUSANorth America
67771From retail ground beefFarmingtonUSAUSANorth America

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Meat

taxonmaps

  • @ref: 69479
  • File name: preview.99_41698.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4110;96_19320;97_23981;98_30315;99_41698&stattab=map
  • Last taxonomy: Peptoniphilus methioninivorax subclade
  • 16S sequence: GU440754
  • Sequence Identity:
  • Total samples: 3189
  • soil counts: 78
  • aquatic counts: 362
  • animal counts: 2708
  • plant counts: 41

Safety information

risk assessment

  • @ref: 16269
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16269
  • description: Peptoniphilus methioninivorax strain NRRL B-23883 16S ribosomal RNA gene, partial sequence
  • accession: GU440754
  • length: 1451
  • database: nuccore
  • NCBI tax ID: 907224

GC content

  • @ref: 16269
  • GC-content: 32.0
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 16269

culture collection no.: DSM 22461, KCTC 15197, NRRL B-23883

straininfo link

  • @ref: 86992
  • straininfo: 397086

literature

  • topic: Phylogeny
  • Pubmed-ID: 20817843
  • title: Peptoniphilus methioninivorax sp. nov., a Gram-positive anaerobic coccus isolated from retail ground beef.
  • authors: Rooney AP, Swezey JL, Pukall R, Schumann P, Spring S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.024232-0
  • year: 2010
  • mesh: Anaerobiosis, Animals, Cattle, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Gram-Positive Cocci/*classification/genetics/*isolation & purification/metabolism, Meat/*microbiology, Molecular Sequence Data, Peptones/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
16269Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22461)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22461
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86992Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID397086.1StrainInfo: A central database for resolving microbial strain identifiers