Strain identifier

BacDive ID: 17982

Type strain: Yes

Species: Howardella ureilytica

Strain history: DSM 15118 <-- T. R. Cook GPC 589.

NCBI tax ID(s): 404403 (species)

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General

@ref: 5719

BacDive-ID: 17982

DSM-Number: 15118

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, coccus-shaped

description: Howardella ureilytica DSM 15118 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from sheep rumen.

NCBI tax id

  • NCBI tax id: 404403
  • Matching level: species

strain history

@refhistory
5719<- A. R. Cook, Health Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury, UK; GPC 589 <- A. R. Cook {1989}
67770DSM 15118 <-- T. R. Cook GPC 589.

doi: 10.13145/bacdive17982.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Howardella
  • species: Howardella ureilytica
  • full scientific name: Howardella ureilytica Cook et al. 2007

@ref: 5719

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Howardella

species: Howardella ureilytica

full scientific name: Howardella ureilytica Cook et al. 2007

type strain: yes

Morphology

cell morphology

  • @ref: 32018
  • gram stain: positive
  • cell length: 0.8 µm
  • cell width: 0.64 µm
  • cell shape: coccus-shaped
  • motility: no

Culture and growth conditions

culture medium

  • @ref: 5719
  • name: HOWARDELLA MEDIUM (DSMZ Medium 1085)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1085
  • composition: Name: HOWARDELLA MEDIUM (DSMZ Medium 1085) Composition: Casitone 20.0 g/l Na2HPO4 5.0 g/l Yeast extract 5.0 g/l MgCl2 x 6 H2O 1.1 g/l Urea 1.0 g/l Na-thioglycolate 0.75 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
5719positivegrowth37mesophilic
32018positiveoptimum37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5719anaerobe
32018anaerobe

spore formation

  • @ref: 32018
  • spore formation: no

observation

  • @ref: 32018
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3201830089acetate+carbon source
3201816199urea+carbon source

enzymes

  • @ref: 32018
  • value: urease
  • activity: +
  • ec: 3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5719sheep rumenUnited KingdomGBREurope
67770Rumen fluid of a sheep

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Caprinae (Sheep/Goat)
#Host Body-Site#Organ#Rumen

taxonmaps

  • @ref: 69479
  • File name: preview.99_134181.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1941;96_9045;97_10875;98_13351;99_134181&stattab=map
  • Last taxonomy: Howardella ureilytica subclade
  • 16S sequence: DQ925472
  • Sequence Identity:
  • Total samples: 17059
  • soil counts: 81
  • aquatic counts: 192
  • animal counts: 16766
  • plant counts: 20

Safety information

risk assessment

  • @ref: 5719
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5719
  • description: Howardella ureilytica strain GPC 589 16S ribosomal RNA gene, partial sequence
  • accession: DQ925472
  • length: 1531
  • database: ena
  • NCBI tax ID: 404403

GC content

@refGC-contentmethod
571934
6777034high performance liquid chromatography (HPLC)

External links

@ref: 5719

culture collection no.: DSM 15118, JCM 13267, GPC 589

straininfo link

  • @ref: 86969
  • straininfo: 323251

literature

  • topic: Phylogeny
  • Pubmed-ID: 18048754
  • title: Howardella ureilytica gen. nov., sp. nov., a Gram-positive, coccoid-shaped bacterium from a sheep rumen.
  • authors: Cook AR, Riley PW, Murdoch H, Evans PN, McDonald IR
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64819-0
  • year: 2007
  • mesh: Adenosine Triphosphate/biosynthesis, Anaerobiosis, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Fermentation, Genes, rRNA, Gram-Positive Cocci/chemistry/*classification/genetics/*isolation & purification, Locomotion, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Rumen/*microbiology, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sheep, Spores, Bacterial/cytology, Urea/metabolism
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
5719Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15118)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15118
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32018Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2827028776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86969Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID323251.1StrainInfo: A central database for resolving microbial strain identifiers