Strain identifier

BacDive ID: 1798

Type strain: Yes

Species: Rhodoblastus acidophilus

Strain Designation: 7050

Strain history: CIP <- 1994, NCIMB <- DSM <- N. Pfennig: strain 7050

NCBI tax ID(s): 1074 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 2116

BacDive-ID: 1798

DSM-Number: 137

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, motile

description: Rhodoblastus acidophilus 7050 is an anaerobe, mesophilic, motile bacterium that was isolated from crystal lake.

NCBI tax id

  • NCBI tax id: 1074
  • Matching level: species

strain history

@refhistory
2116<- N. Pfennig, 7050
116021CIP <- 1994, NCIMB <- DSM <- N. Pfennig: strain 7050

doi: 10.13145/bacdive1798.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Roseiarcaceae
  • genus: Rhodoblastus
  • species: Rhodoblastus acidophilus
  • full scientific name: Rhodoblastus acidophilus (Pfennig 1969) Imhoff 2001

  • synonyms

    • @ref: 20215
    • synonym: Rhodopseudomonas acidophila

@ref: 2116

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Roseiarcaceae

genus: Rhodoblastus

species: Rhodoblastus acidophilus

full scientific name: Rhodoblastus acidophilus (Pfennig 1969) Imhoff 2001

strain designation: 7050

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes91.91
6948099.999negative
116021nonegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2116ACID RHODOSPIRILLACEAE MEDIUM (DSMZ Medium 26)yeshttps://mediadive.dsmz.de/medium/26Name: ACID RHODOSPIRILLACEAE MEDIUM (DSMZ Medium 26) Composition: Disodium succinate 1.0 g/l Ammonium acetate 0.5 g/l KH2PO4 0.5 g/l MgSO4 x 7 H2O 0.4 g/l NaCl 0.4 g/l NH4Cl 0.4 g/l L-Cysteine HCl 0.3 g/l Yeast extract 0.2 g/l CaCl2 x 2 H2O 0.05 g/l Fe(III) citrate 0.005 g/l Resazurin 0.005 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Vitamin B12 Distilled water
38157MEDIUM 121 - for Rhodospeudomonas acidophilayesDistilled water make up to (1000.000 ml);Sodium chloride (0.400 g);Magnesium sulphate heptahydrate (0.400 g);Calcium chloride dihydrate (50.000 mg);Yeast extract (0.200 g);Ferric citrate (5.000 mg);Potassium di-hydrogen phosphate (0.500 g);Ammonium chlori
116021CIP Medium 121yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=121

culture temp

@refgrowthtypetemperaturerange
2116positivegrowth25mesophilic
38157positivegrowth30mesophilic
116021positivegrowth10-30
116021nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 2116
  • oxygen tolerance: anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.992

compound production

  • @ref: 2116
  • compound: hydrogen

halophily

@refsaltgrowthtested relationconcentration
116021NaClpositivegrowth0 %
116021NaClnogrowth2 %
116021NaClnogrowth4 %
116021NaClnogrowth6 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11602116947citrate-carbon source
1160214853esculin-hydrolysis
11602117632nitrate-reduction
11602116301nitrite-reduction
11602115792malonate-assimilation

antibiotic resistance

  • @ref: 116021
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 116021
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
116021oxidase+
116021beta-galactosidase-3.2.1.23
116021alcohol dehydrogenase-1.1.1.1
116021gelatinase-
116021amylase-
116021DNase-
116021catalase-1.11.1.6
116021tween esterase-
116021lysine decarboxylase-4.1.1.18
116021ornithine decarboxylase-4.1.1.17
116021urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116021--++-++---++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
2116crystal lakeIllinoisUSAUSANorth America
116021Environment, LakeCrystal lake, IllinoisUnited States of AmericaUSANorth America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Lake (large)

taxonmaps

  • @ref: 69479
  • File name: preview.99_80840.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_18472;97_22831;98_56244;99_80840&stattab=map
  • Last taxonomy: Rhodoblastus acidophilus subclade
  • 16S sequence: FR733696
  • Sequence Identity:
  • Total samples: 1867
  • soil counts: 758
  • aquatic counts: 693
  • animal counts: 211
  • plant counts: 205

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
21161Risk group (German classification)
1160211Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Rhodoblastus acidophilus partial 16S rRNA gene, type strain DSM137T
  • accession: FR733696
  • length: 1477
  • database: ena
  • NCBI tax ID: 1074

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Rhodoblastus acidophilus DSM 137GCA_900187365contigncbi1074
66792Rhodoblastus acidophilus DSM 137GCA_002937135contigncbi1074
66792Rhodoblastus acidophilus DSM 137GCA_003258765scaffoldncbi1074
66792Rhodoblastus acidophilus strain DSM 1371074.15wgspatric1074
66792Rhodoblastus acidophilus strain DSM 1371074.14wgspatric1074
66792Rhodoblastus acidophilus strain DSM 1371074.16wgspatric1074
66792Rhodoblastus acidophilus DSM 1372831495660draftimg1074
66792Rhodoblastus acidophilus DSM 1372724679731draftimg1074

GC content

  • @ref: 2116
  • GC-content: 65.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno52.044no
gram-positiveno98.843no
anaerobicyes78.28yes
aerobicno84.115no
halophileno97.875no
spore-formingno93.909no
glucose-utilyes72.642no
thermophileno98.369yes
motileyes87.172no
glucose-fermentno90.012no

External links

@ref: 2116

culture collection no.: DSM 137, ATCC 25092, Pfennig 7050, CIP 103962, LMG 4304, NCIMB 11761

straininfo link

  • @ref: 71445
  • straininfo: 4904

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism667181Hydrogen metabolism and nitrogen fixation in wild type and Nif- mutants of Rhodopseudomonas acidophila.Siefert E, Pfennig NBiochimie10.1016/s0300-9084(78)80822-01978Hydrogen/*metabolism, Kinetics, Mutation, *Nitrogen Fixation, Nitrogenase/*metabolism, Rhodopseudomonas/genetics/*metabolism, Species SpecificityEnzymology
Phylogeny16738120Rhodoblastus sphagnicola sp. nov., a novel acidophilic purple non-sulfur bacterium from Sphagnum peat bog.Kulichevskaya IS, Guzev VS, Gorlenko VM, Liesack W, Dedysh SNInt J Syst Evol Microbiol10.1099/ijs.0.63962-02006Bradyrhizobiaceae/*classification/growth & development/isolation & purification/ultrastructure, Culture Media, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Russia, *Soil MicrobiologyCultivation

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2116Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 137)https://www.dsmz.de/collection/catalogue/details/culture/DSM-137
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38157Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15817
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71445Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID4904.1StrainInfo: A central database for resolving microbial strain identifiers
116021Curators of the CIPCollection of Institut Pasteur (CIP 103962)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103962