Strain identifier

BacDive ID: 17972

Type strain: Yes

Species: Anaerococcus vaginalis

Strain history: CIP <- 1993, T. Ezaki, Gifu Univ. School of Med., Gifu, Japan

NCBI tax ID(s): 655811 (strain), 33037 (species)

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General

@ref: 3162

BacDive-ID: 17972

DSM-Number: 7457

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, coccus-shaped

description: Anaerococcus vaginalis CCUG 31349 is an anaerobe, coccus-shaped bacterium that was isolated from ovarian abscess.

NCBI tax id

NCBI tax idMatching level
655811strain
33037species

strain history

@refhistory
3162<- JCM <- T. Ezaki, GIFU 12669
407421993, T. Ezaki, Univ. School Medicine, Gifu, Japan
67771<- CCUG <- JCM <- GIFU 12669
67770GIFU 12669.
123776CIP <- 1993, T. Ezaki, Gifu Univ. School of Med., Gifu, Japan

doi: 10.13145/bacdive17972.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Peptoniphilaceae
  • genus: Anaerococcus
  • species: Anaerococcus vaginalis
  • full scientific name: Anaerococcus vaginalis (Li et al. 1992) Ezaki et al. 2001

  • synonyms

    • @ref: 20215
    • synonym: Peptostreptococcus vaginalis

@ref: 3162

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Peptoniphilaceae

genus: Anaerococcus

species: Anaerococcus vaginalis

full scientific name: Anaerococcus vaginalis (Li et al. 1992) Ezaki et al. 2001

type strain: yes

Morphology

cell morphology

@refcell shapegram stainmotility
67771coccus-shaped
67771positive
123776coccus-shapedpositiveno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3162PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
40742MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
123776CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperature
3162positivegrowth37
40742positivegrowth37
50540positivegrowth37
67770positivegrowth37
67771positivegrowth37

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
3162anaerobe
50540anaerobe
67771anaerobe
123776anaerobe

murein

  • @ref: 3162
  • murein short key: A21.06
  • type: A4ß L-Orn(L-Lys)-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12377617632nitrate+reduction
12377616301nitrite-reduction
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
12377635581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5
123776oxidase-
123776catalase-1.11.1.6
123776urease-3.5.1.5
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
50540-+---+----++--------
123776-----+----+---------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
3162-+--------+-----++---+----+--
50540-+--------+-----++---+--+++--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
3162ovarian abscess
50540Human ovarian abscessJapanJPNAsia
67770Ovarian abscess
67771From human ovarian abscess
123776Human, Ovarian abcessJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Abscess
#Host Body-Site#Urogenital tract#Ovary

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
31622Risk group (German classification)
1237761Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Anaerococcus vaginalis strain CCUG 31349 16S ribosomal RNA gene, partial sequenceAF5422291412nuccore655811
20218Anaerococcus vaginalis gene for 16S ribosomal RNA, partial sequence, strain: JCM 8138AB6915751488nuccore655811
20218Anaerococcus vaginalis gene for 16S ribosomal RNA, partial sequenceD141461375nuccore655811

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerococcus vaginalis ATCC 51170655811.4wgspatric655811
66792Anaerococcus vaginalis strain FDAARGOS_98833037.9completepatric33037
66792Anaerococcus vaginalis ATCC 51170645058761draftimg655811
67770Anaerococcus vaginalis ATCC 51170GCA_000163295scaffoldncbi655811

GC content

@refGC-contentmethod
316230.0high performance liquid chromatography (HPLC)
6777030thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes79.985yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)yes86.204yes
69480spore-formingspore-formingAbility to form endo- or exosporesno71.135no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no97.624yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno94.659yes
69480flagellatedmotile2+Ability to perform flagellated movementno85.012no

External links

@ref: 3162

culture collection no.: CCUG 31349, CIP 103621, KCTC 15028, DSM 7457, ATCC 51170, JCM 8138, GIFU 12669, GIFU 7732

straininfo link

  • @ref: 86959
  • straininfo: 42841

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1390111Three new species of the genus Peptostreptococcus isolated from humans: Peptostreptococcus vaginalis sp. nov., Peptostreptococcus lacrimalis sp. nov., and Peptostreptococcus lactolyticus sp. nov.Li N, Hashimoto Y, Adnan S, Miura H, Yamamoto H, Ezaki TInt J Syst Bacteriol10.1099/00207713-42-4-6021992Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, DNA, Bacterial/chemistry, Fermentation, Humans, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Peptostreptococcus/chemistry/*classification/metabolism, Phenotype, Sequence Homology, Nucleic AcidMetabolism
Phylogeny33987692Anaerococcus faecalis sp. nov., Isolated from Swine Faeces.Yu SY, Oh BS, Ryu SW, Kim JS, Lee JS, Park SH, Kang SW, Lee J, Lee MK, Choe H, Jung WY, Jung H, Hur TY, Kim HB, Kim JK, Lee JH, Lee JHCurr Microbiol10.1007/s00284-021-02497-72021Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, *Fatty Acids/analysis, Feces/chemistry, Firmicutes, Nucleic Acid Hybridization, *Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, SwineTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3162Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7457)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7457
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40742Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15439
50540Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31349)https://www.ccug.se/strain?id=31349
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
86959Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42841.1StrainInfo: A central database for resolving microbial strain identifiers
123776Curators of the CIPCollection of Institut Pasteur (CIP 103621)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103621