Strain identifier

BacDive ID: 17965

Type strain: Yes

Species: Anaerobranca zavarzinii

Strain Designation: JW/VK-KS54, JW/VK-KS5Y

Strain history: <- V. Kevbrin; JW/VK-KS5Y

NCBI tax ID(s): 1120991 (strain), 436000 (species)

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General

@ref: 7725

BacDive-ID: 17965

DSM-Number: 18970

keywords: 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative, rod-shaped

description: Anaerobranca zavarzinii JW/VK-KS54 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from geothermally heated pool.

NCBI tax id

NCBI tax idMatching level
1120991strain
436000species

strain history

  • @ref: 7725
  • history: <- V. Kevbrin; JW/VK-KS5Y

doi: 10.13145/bacdive17965.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Syntrophomonadaceae
  • genus: Anaerobranca
  • species: Anaerobranca zavarzinii
  • full scientific name: Anaerobranca zavarzinii Kevbrin et al. 2008

@ref: 7725

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Proteinivoraceae

genus: Anaerobranca

species: Anaerobranca zavarzinii

full scientific name: Anaerobranca zavarzinii Kevbrin et al. 2008

strain designation: JW/VK-KS54, JW/VK-KS5Y

type strain: yes

Morphology

cell morphology

  • @ref: 32350
  • gram stain: negative
  • cell length: 5.65 µm
  • cell width: 0.4 µm
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

  • @ref: 7725
  • name: ANAEROBRANCA MEDIUM (DSMZ Medium 685)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/685
  • composition: Name: ANAEROBRANCA MEDIUM (DSMZ Medium 685) Composition: Yeast extract 4.97018 g/l Na2HPO4 x 2 H2O 3.87674 g/l Na2-fumarate 1.49105 g/l KH2PO4 0.497018 g/l KCl 0.497018 g/l Na2S x 9 H2O 0.149105 g/l L-Cysteine HCl x H2O 0.149105 g/l MgSO4 x 7 H2O 0.0149105 g/l Nitrilotriacetic acid 0.00745527 g/l NaCl 0.00497018 g/l MnSO4 x H2O 0.00248509 g/l ZnSO4 x 7 H2O 0.000894632 g/l CoSO4 x 7 H2O 0.000894632 g/l FeSO4 x 7 H2O 0.000497018 g/l CaCl2 x 2 H2O 0.000497018 g/l NiCl2 x 6 H2O 0.000149105 g/l AlK(SO4)2 x 12 H2O 9.94036e-05 g/l Pyridoxine hydrochloride 9.94036e-05 g/l Na2MoO4 x 2 H2O 4.97018e-05 g/l Riboflavin 4.97018e-05 g/l (DL)-alpha-Lipoic acid 4.97018e-05 g/l p-Aminobenzoic acid 4.97018e-05 g/l Thiamine HCl 4.97018e-05 g/l Nicotinic acid 4.97018e-05 g/l Calcium D-(+)-pantothenate 4.97018e-05 g/l CuSO4 x 5 H2O 4.97018e-05 g/l H3BO3 4.97018e-05 g/l Folic acid 1.98807e-05 g/l Biotin 1.98807e-05 g/l Na2WO4 x 2 H2O 1.98807e-06 g/l Na2SeO3 x 5 H2O 1.49105e-06 g/l Vitamin B12 9.94036e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7725positivegrowth55thermophilic
32350positivegrowth34-64
32350positiveoptimum57thermophilic

culture pH

@refabilitytypepHPH range
32350positivegrowth7.7-9.9alkaliphile
32350positiveoptimum8.75

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7725anaerobe
32350anaerobe

spore formation

  • @ref: 32350
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
32350NaClpositivegrowth0.12-3.13 %
32350NaClpositiveoptimum1.04 %

observation

  • @ref: 32350
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3235017234glucose+carbon source
3235017306maltose+carbon source
3235015361pyruvate+carbon source
3235017992sucrose+carbon source
3235027082trehalose+carbon source

Isolation, sampling and environmental information

isolation

  • @ref: 7725
  • sample type: geothermally heated pool
  • geographic location: Kamchatka, Pushino
  • country: Russia
  • origin.country: RUS
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Condition#Thermophilic (>45°C)
#Environmental#Aquatic
#Environmental#Terrestrial#Geologic

taxonmaps

  • @ref: 69479
  • File name: preview.99_60003.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3665;96_17455;97_33200;98_42632;99_60003&stattab=map
  • Last taxonomy: Anaerobranca
  • 16S sequence: EF190921
  • Sequence Identity:
  • Total samples: 90
  • soil counts: 14
  • aquatic counts: 29
  • animal counts: 36
  • plant counts: 11

Safety information

risk assessment

  • @ref: 7725
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7725
  • description: Anaerobranca zavarzinii strain JW/VK-KS5Y 16S ribosomal RNA gene, partial sequence
  • accession: EF190921
  • length: 1456
  • database: ena
  • NCBI tax ID: 1120991

GC content

  • @ref: 7725
  • GC-content: 32.5

External links

@ref: 7725

culture collection no.: DSM 18970, VKM B-2436

straininfo link

  • @ref: 86952
  • straininfo: 408041

literature

  • topic: Phylogeny
  • Pubmed-ID: 18523200
  • title: Anaerobranca zavarzinii sp. nov., an anaerobic, alkalithermophilic bacterium isolated from Kamchatka thermal fields.
  • authors: Kevbrin V, Boltyanskaya Y, Garnova E, Wiegel J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65364-0
  • year: 2008
  • mesh: Bacteria, Anaerobic/*classification/genetics/growth & development/*isolation & purification, Bacterial Typing Techniques, Base Composition, Culture Media, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Hot Springs/*microbiology, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Species Specificity
  • topic2: Cultivation

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7725Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18970)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18970
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32350Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2858128776041
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86952Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID408041.1StrainInfo: A central database for resolving microbial strain identifiers