Strain identifier

BacDive ID: 17925

Type strain: Yes

Species: Aquabacterium citratiphilum

Strain Designation: B4

Strain history: CIP <- 2001, W. Manz, Technische Univ. Berlin, Berlin, Germany: strain B4 <- S. Kalmbach

NCBI tax ID(s): 70582 (species)

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General

@ref: 4532

BacDive-ID: 17925

DSM-Number: 11900

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Aquabacterium citratiphilum B4 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from biofilm in drinking water pipe.

NCBI tax id

  • NCBI tax id: 70582
  • Matching level: species

strain history

@refhistory
4532<- S. Kalmbach; B4
118292CIP <- 2001, W. Manz, Technische Univ. Berlin, Berlin, Germany: strain B4 <- S. Kalmbach

doi: 10.13145/bacdive17925.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Comamonadaceae
  • genus: Aquabacterium
  • species: Aquabacterium citratiphilum
  • full scientific name: Aquabacterium citratiphilum Kalmbach et al. 1999

@ref: 4532

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Comamonadaceae

genus: Aquabacterium

species: Aquabacterium citratiphilum

full scientific name: Aquabacterium citratiphilum Kalmbach et al. 1999

strain designation: B4

type strain: yes

Morphology

cell morphology

  • @ref: 118292
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4532PLATE COUNT AGAR (DSMZ Medium 464)yeshttps://mediadive.dsmz.de/medium/464Name: PLATE COUNT AGAR (DSMZ Medium 464) Composition: Agar 15.0 g/l Tryptone 5.0 g/l Yeast extract 2.5 g/l Dextrose 1.0 g/l Distilled water
4532MEDIUM R2A MODIFIED (DSMZ Medium 830a)yeshttps://mediadive.dsmz.de/medium/830aName: MEDIUM R2A MODIFIED (DSMZ Medium 830a) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Tween 80 Distilled water
39629MEDIUM 372 - for AquabacteriumyesDistilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (0.050 g);Agar (15.000 g);Glucose (0.500 g);Yeast extract (0.500 g);Pyruvic acid (0.300g);Tween 80 (1.000 ml);Casamino acids (0.500 g);Proteose Peptone N°3 (0.500 g);Di Potassium mon
118292CIP Medium 372yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=372

culture temp

@refgrowthtypetemperaturerange
4532positivegrowth28mesophilic
39629positivegrowth30mesophilic
60067positivegrowth20-30
118292positivegrowth22-30
118292nogrowth5psychrophilic
118292nogrowth10psychrophilic
118292nogrowth37mesophilic
118292nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 60067
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
118292NaClpositivegrowth0 %
118292NaClnogrowth2 %
118292NaClnogrowth4 %
118292NaClnogrowth6 %
118292NaClnogrowth8 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
11829216947citrate+carbon source
1182924853esculin+hydrolysis
11829217234glucose-fermentation
11829217716lactose+fermentation
11829217632nitrate-reduction
11829216301nitrite-reduction
118292132112sodium thiosulfate-builds gas from

antibiotic resistance

  • @ref: 118292
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11829235581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11829215688acetoin-
11829217234glucose+

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6
118292oxidase-
118292alcohol dehydrogenase-1.1.1.1
118292gelatinase-
118292amylase-
118292DNase-
118292caseinase-3.4.21.50
118292catalase-1.11.1.6
118292tween esterase+
118292gamma-glutamyltransferase-2.3.2.2
118292lecithinase-
118292lipase-
118292lysine decarboxylase-4.1.1.18
118292ornithine decarboxylase-4.1.1.17
118292phenylalanine ammonia-lyase-4.3.1.24
118292tryptophan deaminase-
118292urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118292--++----------------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
4532+---+-----------+++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118292-++++-+--++---+++-++++-++----+++-+--+------++++++++-+++--+-++-+--++--------+---++-+---+++++++++-+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
4532biofilm in drinking water pipeBerlinGermanyDEUEurope
60067Drinking water biofilmBerlinGermanyDEUEurope1996
118292Environment, Drinking water biofilmsGermanyDEUEurope1996

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Environmental#Biofilm

taxonmaps

  • @ref: 69479
  • File name: preview.99_29070.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1;96_1718;97_4766;98_16950;99_29070&stattab=map
  • Last taxonomy: Aquabacterium
  • 16S sequence: AF035050
  • Sequence Identity:
  • Total samples: 1448
  • soil counts: 95
  • aquatic counts: 694
  • animal counts: 614
  • plant counts: 45

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
45321Risk group (German classification)
1182921Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4532
  • description: Beta proteobacterium B4 16S ribosomal RNA gene, partial sequence
  • accession: AF035050
  • length: 1418
  • database: ena
  • NCBI tax ID: 70582

External links

@ref: 4532

culture collection no.: CCUG 53899, ATCC BAA 207, CIP 106985, DSM 11900, CCM 4956

straininfo link

  • @ref: 86914
  • straininfo: 49204

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10319501Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system.Kalmbach S, Manz W, Wecke J, Szewzyk UInt J Syst Bacteriol10.1099/00207713-49-2-7691999Bacterial Proteins/chemistry, Bacterial Typing Techniques, Base Composition, Berlin, *Biofilms, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Genes, rRNA, Gram-Negative Bacteria/*classification/genetics/isolation & purification/physiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Water Microbiology, *Water SupplyGenetics
Phylogeny34582329Aquabacterium soli sp. nov., a novel bacterium isolated from soil under the long-term application of bifenthrin.Sun L, Chen W, Huang K, Lyu W, Gao XInt J Syst Evol Microbiol10.1099/ijsem.0.0047682021Bacterial Typing Techniques, Base Composition, Burkholderiales, China, DNA, Bacterial/genetics, *Fatty Acids/chemistry, Phospholipids/analysis, Phylogeny, Pyrethrins, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *SoilTranscriptome

Reference

@idauthorscataloguedoi/urltitle
4532Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11900)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11900
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39629Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19175
60067Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 53899)https://www.ccug.se/strain?id=53899
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86914Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49204.1StrainInfo: A central database for resolving microbial strain identifiers
118292Curators of the CIPCollection of Institut Pasteur (CIP 106985)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106985