Strain identifier

BacDive ID: 17924

Type strain: Yes

Species: Aquabacterium parvum

Strain Designation: B6

Strain history: CIP <- 2001, W. Manz, Technol. Berlin Univ., Berlin, Germany: strain B6 <- S. Kalmbach

NCBI tax ID(s): 70584 (species)

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General

@ref: 4560

BacDive-ID: 17924

DSM-Number: 11968

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Aquabacterium parvum B6 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from biofilm in drinking water pipe.

NCBI tax id

  • NCBI tax id: 70584
  • Matching level: species

strain history

@refhistory
4560<- S. Kalmbach; B6
121603CIP <- 2001, W. Manz, Technol. Berlin Univ., Berlin, Germany: strain B6 <- S. Kalmbach

doi: 10.13145/bacdive17924.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Comamonadaceae
  • genus: Aquabacterium
  • species: Aquabacterium parvum
  • full scientific name: Aquabacterium parvum Kalmbach et al. 1999

@ref: 4560

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Comamonadaceae

genus: Aquabacterium

species: Aquabacterium parvum

full scientific name: Aquabacterium parvum Kalmbach et al. 1999

strain designation: B6

type strain: yes

Morphology

cell morphology

  • @ref: 121603
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4560MEDIUM R2A MODIFIED (DSMZ Medium 830a)yeshttps://mediadive.dsmz.de/medium/830aName: MEDIUM R2A MODIFIED (DSMZ Medium 830a) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Tween 80 Distilled water
40552MEDIUM 372 - for AquabacteriumyesDistilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (0.050 g);Agar (15.000 g);Glucose (0.500 g);Yeast extract (0.500 g);Pyruvic acid (0.300g);Tween 80 (1.000 ml);Casamino acids (0.500 g);Proteose Peptone N°3 (0.500 g);Di Potassium mon
121603CIP Medium 372yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=372

culture temp

@refgrowthtypetemperaturerange
4560positivegrowth28mesophilic
40552positivegrowth25mesophilic
58080positivegrowth30mesophilic
121603positivegrowth15-37
121603nogrowth5psychrophilic
121603nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 58080
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
121603NaClnogrowth0 %
121603NaClnogrowth2 %
121603NaClnogrowth4 %
121603NaClnogrowth6 %
121603NaClnogrowth8 %
121603NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1216034853esculin-hydrolysis
12160317632nitrate+reduction
12160316301nitrite-reduction
12160317234glucose-degradation

antibiotic resistance

  • @ref: 121603
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 121603
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12160315688acetoin-
12160317234glucose-

enzymes

@refvalueactivityec
121603oxidase+
121603alcohol dehydrogenase-1.1.1.1
121603gelatinase-
121603amylase-
121603catalase-1.11.1.6
121603gamma-glutamyltransferase-2.3.2.2
121603lysine decarboxylase-4.1.1.18
121603ornithine decarboxylase-4.1.1.17
121603phenylalanine ammonia-lyase-4.3.1.24
121603tryptophan deaminase-
121603urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121603--++-+-----+--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121603------------+-------------------+-------------------------------------------------------+----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
4560biofilm in drinking water pipeBerlinGermanyDEUEurope
58080Drinking water biofilmsGermanyDEUEurope1996
121603Environment, Drinking water biofilmsGermanyDEUEurope1996

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Environmental#Biofilm

taxonmaps

  • @ref: 69479
  • File name: preview.99_10995.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1;96_2647;97_3245;98_4079;99_10995&stattab=map
  • Last taxonomy: Aquabacterium
  • 16S sequence: AF035052
  • Sequence Identity:
  • Total samples: 2309
  • soil counts: 219
  • aquatic counts: 1271
  • animal counts: 676
  • plant counts: 143

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
45601Risk group (German classification)
1216031Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4560
  • description: Beta proteobacterium B6 16S ribosomal RNA gene, partial sequence
  • accession: AF035052
  • length: 1416
  • database: ena
  • NCBI tax ID: 70584

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aquabacterium parvum B6GCA_001447195contigncbi70584
66792Aquabacterium parvum B62684622550draftimg70584

External links

@ref: 4560

culture collection no.: DSM 11968, CCM 4958, CCUG 48317, CIP 106983, ATCC BAA 208

straininfo link

  • @ref: 86913
  • straininfo: 49208

literature

  • topic: Phylogeny
  • Pubmed-ID: 10319501
  • title: Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system.
  • authors: Kalmbach S, Manz W, Wecke J, Szewzyk U
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-49-2-769
  • year: 1999
  • mesh: Bacterial Proteins/chemistry, Bacterial Typing Techniques, Base Composition, Berlin, *Biofilms, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Genes, rRNA, Gram-Negative Bacteria/*classification/genetics/isolation & purification/physiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Water Microbiology, *Water Supply
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
4560Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11968)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11968
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40552Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19173
58080Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48317)https://www.ccug.se/strain?id=48317
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86913Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49208.1StrainInfo: A central database for resolving microbial strain identifiers
121603Curators of the CIPCollection of Institut Pasteur (CIP 106983)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106983