General

@ref: 6090

BacDive-ID: 17901

DSM-Number: 15733

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Methylohalomonas lacus DSM 15733 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from hypersaline inland lakes in south-western Siberia.

NCBI tax id

• NCBI tax id: 398773
• Matching level: species

strain history

• @ref: 6090
• history: <- D. Y. Sorokin, Winogradski Inst. Microbiol., RAS, Moscow, Russia; HMT 1

doi: 10.13145/bacdive17901.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/pseudomonadota
• domain: Bacteria
• phylum: Pseudomonadota
• class: Gammaproteobacteria
• order: Chromatiales
• family: Ectothiorhodospiraceae
• genus: Methylohalomonas
• species: Methylohalomonas lacus
• full scientific name: Methylohalomonas lacus Sorokin et al. 2007

@ref: 6090

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Ectothiorhodospiraceae

genus: Methylohalomonas

species: Methylohalomonas lacus

full scientific name: Methylohalomonas lacus Sorokin et al. 2007

type strain: yes

Morphology

cell morphology

• @ref: 32094
• gram stain: negative
• cell length: 2 µm
• cell width: 0.55 µm
• cell shape: rod-shaped
• motility: no

Culture and growth conditions

culture medium

• @ref: 6090
• name: METHYLOHALOMONAS MEDIUM (DSMZ Medium 1171)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1171
• composition: Name: METHYLOHALOMONAS MEDIUM (DSMZ Medium 1171) Composition: NaCl 120.0 g/l K2HPO4 2.0 g/l NaHCO3 1.7 g/l Methanol 1.584 g/l MgSO4 x 7 H2O 0.5 g/l NH4Cl 0.5 g/l NaS2O3 x 5 H2O 0.024 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l Vitamin B12 0.0001 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water

culture temp

• @ref: 6090
• growth: positive
• type: growth
• temperature: 28
• range: mesophilic

culture pH

Physiology and metabolism

oxygen tolerance

• @ref: 32094
• oxygen tolerance: aerobe

halophily

observation

• @ref: 32094
• observation: aggregates in chains

metabolite utilization

Isolation, sampling and environmental information

isolation

• @ref: 6090
• sample type: hypersaline inland lakes in south-western Siberia
• geographic location: Altai
• country: Russia
• origin.country: RUS
• continent: Asia

isolation source categories

Safety information

risk assessment

• @ref: 6090
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 6090
• description: Methylohalomonas lacus strain HMT 1 16S ribosomal RNA gene, partial sequence
• accession: DQ834966
• length: 1325
• database: ena
• NCBI tax ID: 398773

GC content

• @ref: 6090
• GC-content: 59.6

External links

@ref: 6090

culture collection no.: DSM 15733, NCCB 100208, UNIQEM U237, HMT 1

straininfo link

• @ref: 86890
• straininfo: 408381

literature

• topic: Metabolism
• Pubmed-ID: 18048722
• title: Methylohalomonas lacus gen. nov., sp. nov. and Methylonatrum kenyense gen. nov., sp. nov., methylotrophic gammaproteobacteria from hypersaline lakes.
• authors: Sorokin DY, Trotsenko YA, Doronina NV, Tourova TP, Galinski EA, Kolganova TV, Muyzer G
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijs.0.64955-0
• year: 2007
• mesh: Aerobiosis/physiology, Betaine/analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Dimethylamines/metabolism, Ectothiorhodospiraceae/genetics, Ethanol/metabolism, Formaldehyde/metabolism, Formates/metabolism, Gammaproteobacteria/chemistry/*classification/genetics/*isolation & purification, Genes, rRNA, Hydrogen-Ion Concentration, Kenya, Methanol/*metabolism, Methylamines/metabolism, Molecular Sequence Data, Oxidation-Reduction, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, *Water Microbiology
• topic2: Phylogeny

Reference