Strain identifier

BacDive ID: 1789

Type strain: Yes

Species: Bosea massiliensis

Strain Designation: 63287

Strain history: CIP <- 1999, B. La Scola, Timone Hosp., Marseille, France: strain 63287

NCBI tax ID(s): 151419 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 15546

BacDive-ID: 1789

DSM-Number: 21079

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Bosea massiliensis 63287 is a microaerophile, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from water, hospital water supply.

NCBI tax id

  • NCBI tax id: 151419
  • Matching level: species

strain history

@refhistory
15546<- CCUG <- B. La Scola, Univ. Marseille
386181999, B. La Scola, Fac. Med., Marseille, France: strain 63287
120850CIP <- 1999, B. La Scola, Timone Hosp., Marseille, France: strain 63287

doi: 10.13145/bacdive1789.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Boseaceae
  • genus: Bosea
  • species: Bosea massiliensis
  • full scientific name: Bosea massiliensis La Scola et al. 2003

@ref: 15546

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Boseaceae

genus: Bosea

species: Bosea massiliensis

full scientific name: Bosea massiliensis La Scola et al. 2003

strain designation: 63287

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityflagellum arrangement
23324negativerod-shapedyesmonotrichous, polar
120850negativerod-shapedyes

colony morphology

  • @ref: 23324
  • type of hemolysis: alpha
  • hemolysis ability: 1
  • colony color: cream
  • colony shape: circular
  • incubation period: 2-3 days
  • medium used: BCYE agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15546BOSEA MEDIUM (DSMZ Medium 1052)yeshttps://mediadive.dsmz.de/medium/1052Name: BOSEA MEDIUM (DSMZ Medium 1052) Composition: Agar 15.0 g/l ACES 10.0 g/l Yeast extract 10.0 g/l Activated charcoal 2.0 g/l Distilled water
15546R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
23324BCYE agaryes
38618MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
120850CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
15546positivegrowth28mesophilic
23324positivegrowth25mesophilic
23324positivegrowth37mesophilic
23324nogrowth42thermophilic
38618positivegrowth30mesophilic
55674positivegrowth30mesophilic
120850positivegrowth22-30
120850nogrowth15psychrophilic
120850nogrowth37mesophilic
120850nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
15546microaerophile
55674aerobe

halophily

  • @ref: 23324
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2332422599arabinose-assimilation
2332416947citrate-assimilation
2332417634D-glucose-assimilation
2332417306maltose-assimilation
2332429864mannitol-assimilation
2332437684mannose-assimilation
23324506227N-acetylglucosamine-assimilation
2332418401phenylacetate-assimilation
23324168082-dehydro-D-gluconate-builds acid from
23324174265-dehydro-D-gluconate-builds acid from
2332427613amygdalin-builds acid from
2332418305arbutin-builds acid from
2332415963ribitol-builds acid from
2332417108D-arabinose-builds acid from
2332418333D-arabitol-builds acid from
2332417057cellobiose-builds acid from
2332415824D-fructose-builds acid from
2332428847D-fucose-builds acid from
2332412936D-galactose-builds acid from
2332417634D-glucose-builds acid from
2332417716lactose-builds acid from
2332462318D-lyxose-builds acid from
2332417306maltose-builds acid from
2332416899D-mannitol-builds acid from
2332416024D-mannose-builds acid from
233246731melezitose-builds acid from
2332428053melibiose-builds acid from
2332416634raffinose-builds acid from
2332416988D-ribose-builds acid from
2332417992sucrose-builds acid from
2332417924D-sorbitol-builds acid from
2332416443D-tagatose-builds acid from
2332416551D-trehalose-builds acid from
2332432528turanose-builds acid from
2332465327D-xylose-builds acid from
2332416813galactitol-builds acid from
2332417113erythritol-builds acid from
233244853esculin-builds acid from
2332428066gentiobiose-builds acid from
2332424265gluconate-builds acid from
2332417754glycerol-builds acid from
2332428087glycogen-builds acid from
2332417268myo-inositol-builds acid from
2332415443inulin-builds acid from
2332430849L-arabinose-builds acid from
2332418403L-arabitol-builds acid from
2332418287L-fucose-builds acid from
2332462345L-rhamnose-builds acid from
2332417266L-sorbose-builds acid from
2332465328L-xylose-builds acid from
23324320061methyl alpha-D-glucopyranoside-builds acid from
2332443943methyl alpha-D-mannoside-builds acid from
2332474863methyl beta-D-xylopyranoside-builds acid from
23324506227N-acetylglucosamine-builds acid from
2332417814salicin-builds acid from
2332428017starch-builds acid from
2332417151xylitol-builds acid from
233244853esculin-hydrolysis
233245291gelatin-hydrolysis
2332416094thiosulfate-oxidation
2332417128adipate+assimilation
2332424265gluconate+assimilation
2332425115malate+assimilation
2332417632nitrate+reduction
12085017632nitrate+reduction
12085016301nitrite-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.group ID
2332429007ceftriaxoneyesyes0.25 µg/mL (MIC)
2332450845doxycyclineyesyes0.25 µg/mL (MIC)
233249587ticarcillinyesyes0.25 µg/mL (MIC)
233242637amikacinyesyes0.5 µg/mL (MIC)
2332448923erythromycinyesyes0.5 µg/mL (MIC)
2332428077rifampicinyesyes0.5 µg/mL (MIC)
23324478164cefepimeyesyes1 µg/mL (MIC)
233243542cephalothinyesyes1 µg/mL (MIC)
23324471744imipenemyesyes1 µg/mL (MIC)
2332437943colistinyesyes128 µg/mL (MIC)
233248232piperacillinyesyes16 µg/mL (MIC)
233242676amoxicillinyesyes2 µg/mL (MIC)14
23324209807cefoxitinyesyes2 µg/mL (MIC)
23324100241ciprofloxacinyesyes2 µg/mL (MIC)
2332428864tobramycinyesyes2 µg/mL (MIC)
233243770co-trimoxazoleyesyes256 µg/mL (MIC)
2332428001vancomycinyesyes256 µg/mL (MIC)
2332417833gentamicinyesyes4 µg/mL (MIC)
233242676amoxicillinyesyes64 µg/mL (MIC)
2332417334penicillinyesyes64 µg/mL (MIC)
233243508ceftazidimeyesyes8 µg/mL (MIC)
233247528netilmycinyesyes8 µg/mL (MIC)
2332448947clavulanic acidyesyes2 µg/mL (MIC)14

metabolite production

  • @ref: 120850
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
23324arginine dihydrolase-3.5.3.6
23324beta-galactosidase-3.2.1.23
23324catalase+1.11.1.6
23324cytochrome oxidase+1.9.3.1
23324urease+3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120850oxidase+
120850alcohol dehydrogenase-1.1.1.1
120850gelatinase-
120850catalase+1.11.1.6
120850lysine decarboxylase-4.1.1.18
120850ornithine decarboxylase-4.1.1.17
120850urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120850-+++-+--+-++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
15546water, hospital water supplyMarseilleFranceFRAEurope
55674Water,hospital water supplyMarseilleFranceFRAEurope1999
120850Environment, Water tankMarseilleFranceFRAEurope1999

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic
#Infection#Medical environment#Clinic

taxonmaps

  • @ref: 69479
  • File name: preview.99_3433.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_1319;97_1566;98_1905;99_3433&stattab=map
  • Last taxonomy: Bosea
  • 16S sequence: AF288309
  • Sequence Identity:
  • Total samples: 3817
  • soil counts: 1018
  • aquatic counts: 1490
  • animal counts: 1033
  • plant counts: 276

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
155461Risk group (German classification)
1208501Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15546
  • description: Alpha proteobacterium 63287 16S ribosomal RNA gene, partial sequence
  • accession: AF288309
  • length: 1431
  • database: ena
  • NCBI tax ID: 1197712

GC content

@refGC-contentmethod
2332466.7high performance liquid chromatography (HPLC)
2332465.0high performance liquid chromatography (HPLC)

External links

@ref: 15546

culture collection no.: DSM 21079, CCUG 43117, CIP 106336

straininfo link

  • @ref: 71436
  • straininfo: 100566

literature

  • topic: Phylogeny
  • Pubmed-ID: 12656146
  • title: Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996).
  • authors: La Scola B, Mallet MN, Grimont PAD, Raoult D
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02127-0
  • year: 2003
  • mesh: Alphaproteobacteria/*classification/genetics/isolation & purification/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Hospitals, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, *Water Microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15546Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21079)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21079
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23324Bernard La Scola, Marie-Noelle Mallet, Patrick A. D. Grimont, Didier Raoult10.1099/ijs.0.02127-0Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996)IJSEM 53: 15-20 200312656146
38618Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18455
55674Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 43117)https://www.ccug.se/strain?id=43117
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71436Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100566.1StrainInfo: A central database for resolving microbial strain identifiers
120850Curators of the CIPCollection of Institut Pasteur (CIP 106336)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106336