Strain identifier

BacDive ID: 17825

Type strain: Yes

Species: Jiangella muralis

Strain Designation: 15-Je-017

Strain history: CCM 7680 <-- P. Kämpfer 15-Je-017.

NCBI tax ID(s): 702383 (species)

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General

@ref: 16348

BacDive-ID: 17825

DSM-Number: 45357

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, motile, rod-shaped

description: Jiangella muralis 15-Je-017 is an aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from mouldy cellar wall of a house.

NCBI tax id

  • NCBI tax id: 702383
  • Matching level: species

strain history

@refhistory
16348<- P. Kämpfer, Univ. Giessen, Germany; 15-Je-017
67770CCM 7680 <-- P. Kämpfer 15-Je-017.

doi: 10.13145/bacdive17825.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Jiangellales
  • family: Jiangellaceae
  • genus: Jiangella
  • species: Jiangella muralis
  • full scientific name: Jiangella muralis Kämpfer et al. 2011

@ref: 16348

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Jiangellaceae

genus: Jiangella

species: Jiangella muralis

full scientific name: Jiangella muralis Kämpfer et al. 2011 emend. Nouioui et al. 2018

strain designation: 15-Je-017

type strain: yes

Morphology

cell morphology

@refgram staincell widthcell shapemotilityconfidence
29841positive1.3 µmrod-shapedyes
69480positive100

colony morphology

@refincubation periodmedium usedcolony color
2139510-14 daysISP 3
2139510-14 daysISP 4Ivory (1014)
2139510-14 daysISP 5Light Ivory (1015)
2139510-14 daysISP 7Light Ivory (1014)
2139510-14 daysSuter with tyrosineLight ivory (1015)
2139510-14 daysSuter without tyrosineLight ivory (1015)
615373 days

multicellular morphology

@refforms multicellular complexmedium namecomplex namecomplex color
21395noISP 3
21395yesISP 4Aerial MyceliumLight ivory (1015)
21395noISP 5
21395noISP 7
21395noSuter with tyrosine
21395noSuter without tyrosine

pigmentation

  • @ref: 29841
  • production: yes

multimedia

  • @ref: 16348
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45357.jpg
  • caption: Medium 535 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16348GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
16348TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
16348GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
21395ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21395ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
21395ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
21395ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
21395Suter with tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
21395Suter without tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
16348positivegrowth28mesophilic
29841positiveoptimum27.5mesophilic
61537positivegrowth30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29841aerobe
61537aerobe

spore formation

@refspore formationconfidence
29841no
69481yes95

halophily

  • @ref: 21395
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2139517234glucose-
2139522599arabinose+
2139517992sucrose+
2139518222xylose+
2139517268myo-inositol+
2139537684mannose+
2139528757fructose+
2139526546rhamnose-
2139516634raffinose+
2139562968cellulose+
29841370543-hydroxybutyrate+carbon source
2984130089acetate+carbon source
2984115963ribitol+carbon source
2984116449alanine+carbon source
2984122599arabinose+carbon source
2984135391aspartate+carbon source
2984117057cellobiose+carbon source
2984128757fructose+carbon source
2984128260galactose+carbon source
2984117234glucose+carbon source
2984127570histidine+carbon source
2984124996lactate+carbon source
2984125115malate+carbon source
2984117306maltose+carbon source
2984129864mannitol+carbon source
2984137684mannose+carbon source
29841506227N-acetylglucosamine+carbon source
2984128044phenylalanine+carbon source
2984126271proline+carbon source
2984115361pyruvate+carbon source
2984126546rhamnose+carbon source
2984133942ribose+carbon source
2984117814salicin+carbon source
2984117992sucrose+carbon source
2984127082trehalose+carbon source
2984118222xylose+carbon source
298414853esculin+hydrolysis
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
21395--+/-+-+++--+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
21395++/-+-+++/-+/--++++-++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
16348mouldy cellar wall of a houseJenaGermanyDEUEurope
61537House cellar wall colonized with mouldsJenaGermanyDEUEurope2007
67770Mouldy cellar wall of a houseJenaGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#House
#Host#Fungi

taxonmaps

  • @ref: 69479
  • File name: preview.99_3983.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_94;96_2023;97_2438;98_3015;99_3983&stattab=map
  • Last taxonomy: Jiangella
  • 16S sequence: FN645214
  • Sequence Identity:
  • Total samples: 542
  • soil counts: 468
  • aquatic counts: 23
  • animal counts: 40
  • plant counts: 11

Safety information

risk assessment

  • @ref: 16348
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16348
  • description: Jiangella muralis partial 16S rRNA gene, tye strain 15-Je-017T
  • accession: FN645214
  • length: 1363
  • database: ena
  • NCBI tax ID: 702383

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Jiangella muralis strain DSM 45357702383.7wgspatric702383
66792Jiangella muralis DSM 453572654587582draftimg702383
67770Jiangella muralis DSM 45357GCA_001270745scaffoldncbi702383

GC content

  • @ref: 67770
  • GC-content: 72.3
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes95no
gram-positiveyes92.823yes
anaerobicno98.934yes
halophileno90.247yes
spore-formingno52.272no
glucose-utilyes88.773no
flagellatedno97.871no
thermophileno96.707yes
motileno81.102yes
aerobicyes90.746no
glucose-fermentno87.588yes

External links

@ref: 16348

culture collection no.: DSM 45357, CCM 7680, CCUG 57954, JCM 17970

straininfo link

  • @ref: 86816
  • straininfo: 401789

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20173006Jiangella muralis sp. nov., from an indoor environment.Kampfer P, Schafer J, Lodders N, Martin KInt J Syst Evol Microbiol10.1099/ijs.0.022277-02010Actinomycetales/chemistry/*classification/genetics/*isolation & purification, Cell Wall/chemistry, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, *Environmental Microbiology, Fatty Acids/analysis, Glucose/analysis, Molecular Sequence Data, Mycolic Acids/analysis, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Rhamnose/analysis, Sequence Analysis, DNAGenetics
Phylogeny25948618Jiangella mangrovi sp. nov., isolated from mangrove soil.Suksaard P, Duangmal K, Srivibool R, Xie Q, Hong K, Pathom-Aree WInt J Syst Evol Microbiol10.1099/ijs.0.0003032015Actinomycetales/*classification/genetics/isolation & purification, Avicennia/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny30556802Jiangella endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans.Niemhom N, Chutrakul C, Suriyachadkun C, Tadtong S, Thawai CInt J Syst Evol Microbiol10.1099/ijsem.0.0031752018Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Base Sequence, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizome/*microbiology, Sequence Analysis, DNA, Thailand, Vitamin K 2/analogs & derivatives/chemistry, Zingiberaceae/*microbiologyTranscriptome
Phylogeny30603802Jiangella anatolica sp. nov. isolated from coastal lake soil.Ay H, Nouioui I, Carro L, Klenk HP, Cetin D, Igual JM, Sahin N, Isik KAntonie Van Leeuwenhoek10.1007/s10482-018-01222-y2019Actinobacteria/classification/genetics/*isolation & purification/metabolism, Bacterial Typing Techniques, Base Composition, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Geologic Sediments/*microbiology, Lakes/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, TurkeyMetabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16348Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45357)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45357
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
21395Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45357.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
29841Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2621728776041
61537Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 57954)https://www.ccug.se/strain?id=57954
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86816Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID401789.1StrainInfo: A central database for resolving microbial strain identifiers