Strain identifier

BacDive ID: 17825

Type strain: Yes

Species: Jiangella muralis

Strain Designation: 15-Je-017

Culture col. no.: DSM 45357, CCM 7680, CCUG 57954

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General

@ref: 16348

BacDive-ID: 17825

DSM-Number: 45357

keywords: gram-positive, mesophilic, aerobe, Bacteria, 16S sequence, genome sequence, motile, rod-shaped

description: Jiangella muralis 15-Je-017 is an aerobe, mesophilic, gram-positive bacterium that builds an aerial mycelium and was isolated from mouldy cellar wall of a house.

strain history: <- P. Kämpfer, Univ. Giessen, Germany; 15-Je-017

doi: 10.13145/bacdive17825.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Jiangellales
  • family: Jiangellaceae
  • genus: Jiangella
  • species: Jiangella muralis
  • full scientific name: Jiangella muralis Kämpfer et al. 2011

@ref: 16348

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Jiangellaceae

genus: Jiangella

species: Jiangella muralis

full scientific name: Jiangella muralis Kämpfer et al. 2011 emend. Nouioui et al. 2018

strain designation: 15-Je-017

type strain: yes

Morphology

cell morphology

  • @ref: 29841
  • gram stain: positive
  • cell width: 1.3 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

@refincubation periodmedium usedcolony color
2139510-14 daysISP 3
2139510-14 daysISP 4Ivory (1014)
2139510-14 daysISP 5Light Ivory (1015)
2139510-14 daysISP 7Light Ivory (1014)
2139510-14 daysSuter with tyrosineLight ivory (1015)
2139510-14 daysSuter without tyrosineLight ivory (1015)
615373 days

multicellular morphology

@refforms multicellular complexmedium namecomplex namecomplex color
21395noISP 3
21395yesISP 4Aerial MyceliumLight ivory (1015)
21395noISP 5
21395noISP 7
21395noSuter with tyrosine
21395noSuter without tyrosine

multimedia

  • @ref: 16348
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45357.jpg
  • caption: Medium 535 28°C
  • license or copyright: (C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  • intellectual property rights: © Leibniz-Institut DSMZ

pigmentation

  • @ref: 29841
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16348GPHF-MEDIUM (DSMZ Medium 553)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium553.pdf
16348TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium535.pdf
21395ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21395ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
21395ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
21395ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
21395Suter with tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
21395Suter without tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
16348positivegrowth28mesophilic
29841positiveoptimum27.5mesophilic
61537positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29841aerobe
61537aerobe

spore formation

  • @ref: 29841
  • spore formation: no

halophily

  • @ref: 21395
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2139517234glucose-
2139522599arabinose+
2139517992sucrose+
2139518222xylose+
2139517268myo-inositol+
2139537684mannose+
2139528757fructose+
2139526546rhamnose-
2139516634raffinose+
2139562968cellulose+
29841370543-hydroxybutyrate+carbon source
2984130089acetate+carbon source
2984115963ribitol+carbon source
2984116449alanine+carbon source
2984122599arabinose+carbon source
2984135391aspartate+carbon source
2984117057cellobiose+carbon source
2984128757fructose+carbon source
2984128260galactose+carbon source
2984117234glucose+carbon source
2984127570histidine+carbon source
2984124996lactate+carbon source
2984125115malate+carbon source
2984117306maltose+carbon source
2984129864mannitol+carbon source
2984137684mannose+carbon source
29841506227N-acetylglucosamine+carbon source
2984128044phenylalanine+carbon source
2984126271proline+carbon source
2984115361pyruvate+carbon source
2984126546rhamnose+carbon source
2984133942ribose+carbon source
2984117814salicin+carbon source
2984117992sucrose+carbon source
2984127082trehalose+carbon source
2984118222xylose+carbon source
298414853esculin+hydrolysis

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
21395--+/-+-+++--+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
21395++/-+-+++/-+/--++++-++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
16348mouldy cellar wall of a houseJenaGermanyDEUEurope
61537House cellar wall colonized with mouldsJenaGermanyDEUEurope2007

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#House
#Host#Fungi

Safety information

risk assessment

  • @ref: 16348
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16348
  • description: Jiangella muralis partial 16S rRNA gene, tye strain 15-Je-017T
  • accession: FN645214
  • length: 1363
  • database: ena
  • NCBI tax ID: 702383

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Jiangella muralis DSM 45357GCA_001270745scaffoldncbi702383
66792Jiangella muralis strain DSM 45357702383.7wgspatric702383

External links

@ref: 16348

culture collection no.: DSM 45357, CCM 7680, CCUG 57954

straininfo link

@refpassport
20218http://www.straininfo.net/strains/863826
20218http://www.straininfo.net/strains/863825

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
16348Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45357)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45357
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
21395Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45357.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
2621710.1099/ijs.0.022277-0
29841Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information26217
61537Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 57954)https://www.ccug.se/strain?id=57954
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)