Strain identifier

BacDive ID: 17788

Type strain: Yes

Species: Salinisphaera hydrothermalis

Strain history: C. Vetriani EPR70.

NCBI tax ID(s): 563188 (species)

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General

@ref: 15734

BacDive-ID: 17788

DSM-Number: 21483

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Salinisphaera hydrothermalis DSM 21483 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from diffuse-flow hydrothermal vent fluids.

NCBI tax id

  • NCBI tax id: 563188
  • Matching level: species

strain history

@refhistory
15734<- C. Vetriani, Inst. Marine and Coastal Sci., Rutgers, State Univ. New Jersey, New Brunswick, USA; EPR70 <- A. Chatziefthimiou
67770C. Vetriani EPR70.

doi: 10.13145/bacdive17788.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Nevskiales
  • family: Salinisphaeraceae
  • genus: Salinisphaera
  • species: Salinisphaera hydrothermalis
  • full scientific name: Salinisphaera hydrothermalis Crespo-Medina et al. 2009

@ref: 15734

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Salinisphaerales, not validated

family: Salinisphaeraceae

genus: Salinisphaera

species: Salinisphaera hydrothermalis

full scientific name: Salinisphaera hydrothermalis Crespo-Medina et al. 2009

type strain: yes

Morphology

cell morphology

  • @ref: 29053
  • gram stain: negative
  • cell length: 0.9 µm
  • cell width: 0.4 µm
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

  • @ref: 15734
  • name: SALINISPHAERA HYDROTHERMALIS MEDIUM (DSMZ Medium 1216)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1216
  • composition: Name: SALINISPHAERA HYDROTHERMALIS MEDIUM (DSMZ Medium 1216) Composition: NaCl 24.0 g/l Agar 15.0 g/l MgCl2 x 6 H2O 7.0 g/l Yeast extract 3.0 g/l Bacto peptone 2.5 g/l KCl 0.7 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15734positivegrowth28mesophilic
29053positivegrowth20-40
29053positiveoptimum32.5mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
29053positivegrowth5.0-7.5
29053positiveoptimum5.5

Physiology and metabolism

oxygen tolerance

  • @ref: 29053
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29053
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
29053NaClpositivegrowth01-25 %
29053NaClpositiveoptimum2.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2905315963ribitol+carbon source
2905316449alanine+carbon source
2905318403L-arabitol+carbon source
2905328757fructose+carbon source
2905328260galactose+carbon source
2905317234glucose+carbon source
2905317754glycerol+carbon source
2905324996lactate+carbon source
2905329864mannitol+carbon source
2905337684mannose+carbon source
2905326271proline+carbon source
2905351850methyl pyruvate+carbon source
2905326546rhamnose+carbon source
2905330911sorbitol+carbon source
2905330031succinate+carbon source
2905353424tween 20+carbon source
2905353426tween 80+carbon source
2905317151xylitol+carbon source

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationlatitudelongitude
15734diffuse-flow hydrothermal vent fluidsEast Pacific Rise (9° 50' N, 104° 17' W)9.8333-104.283
67770Diffuse-flow hydrothermal vent fluid collected from the East Pacific Rise

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Hydrothermal vent
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_2931.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_357;96_1546;97_1845;98_2265;99_2931&stattab=map
  • Last taxonomy: Salinisphaera hydrothermalis subclade
  • 16S sequence: EU740416
  • Sequence Identity:
  • Total samples: 1134
  • soil counts: 73
  • aquatic counts: 938
  • animal counts: 108
  • plant counts: 15

Safety information

risk assessment

  • @ref: 15734
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15734
  • description: Salinisphaera hydrothermalis strain EPR70 16S ribosomal RNA gene, partial sequence
  • accession: EU740416
  • length: 1376
  • database: ena
  • NCBI tax ID: 1304273

GC content

@refGC-contentmethod
1573464.0high performance liquid chromatography (HPLC)
1573469.0
2905364
6777064high performance liquid chromatography (HPLC)

External links

@ref: 15734

culture collection no.: DSM 21483, JCM 15514, EPR 70

straininfo link

  • @ref: 86779
  • straininfo: 406389

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19502342Salinisphaera hydrothermalis sp. nov., a mesophilic, halotolerant, facultatively autotrophic, thiosulfate-oxidizing gammaproteobacterium from deep-sea hydrothermal vents, and emended description of the genus Salinisphaera.Crespo-Medina M, Chatziefthimiou A, Cruz-Matos R, Perez-Rodriguez I, Barkay T, Lutz RA, Starovoytov V, Vetriani CInt J Syst Evol Microbiol10.1099/ijs.0.005058-02009Base Composition, *Chemoautotrophic Growth, Cytochrome P-450 CYP4A/genetics, DNA, Bacterial/analysis, Gammaproteobacteria/*classification/genetics/isolation & purification/physiology, *Hot Temperature, Hydrogen-Ion Concentration, Molecular Sequence Data, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Ribulose-Bisphosphate Carboxylase/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Sodium Chloride/*metabolism, Species Specificity, Thiosulfates/*metabolismStress
Phylogeny19656943Salinisphaera dokdonensis sp. nov., isolated from surface seawater.Bae GD, Hwang CY, Kim HM, Cho BCInt J Syst Evol Microbiol10.1099/ijs.0.010058-02009DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Gammaproteobacteria/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiologyMetabolism
Phylogeny21984663Salinisphaera orenii sp. nov., isolated from a solar saltern.Park SJ, Cha IT, Kim SJ, Shin KS, Hong Y, Roh DH, Rhee SKInt J Syst Evol Microbiol10.1099/ijs.0.028647-02011Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Gammaproteobacteria/*classification/genetics/isolation & purification, Geologic Sediments/microbiology, Molecular Sequence Data, Phenotype, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analysis, *Water MicrobiologyGenetics
Phylogeny22058321Salinisphaera halophila sp. nov., a moderately halophilic bacterium isolated from brine of a salt well.Zhang YJ, Tang SK, Shi R, Klenk HP, Chen C, Yang LL, Zhou Y, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.035584-02011Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Gammaproteobacteria/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, Quinones/analysis, Salts/analysis, Sequence Analysis, DNA, *Water MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15734Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21483)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21483
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29053Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2548328776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86779Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID406389.1StrainInfo: A central database for resolving microbial strain identifiers