General

@ref: 4934

BacDive-ID: 17652

DSM-Number: 13639

keywords: 16S sequence, Bacteria, anaerobe, thermophilic

description: Thermolithobacter ferrireducens JW/KA-2 is an anaerobe, thermophilic bacterium that was isolated from mixed sample of geothermally heated sediment and water.

NCBI tax id

• NCBI tax id: 416555
• Matching level: species

strain history

• @ref: 4934
• history: <- J. Wiegel; JW/KA-2 (Ferribacter thermoautotrophicus) <- J. Hanel {1999}

doi: 10.13145/bacdive17652.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/bacillota
• domain: Bacteria
• phylum: Bacillota
• class: Thermolithobacteria
• order: Thermolithobacterales
• family: Thermolithobacteraceae
• genus: Thermolithobacter
• species: Thermolithobacter ferrireducens
• full scientific name: Thermolithobacter ferrireducens Sokolova et al. 2007

@ref: 4934

domain: Bacteria

phylum: Firmicutes

class: Thermolithobacteria

order: Thermolithobacterales

family: Thermolithobacteraceae

genus: Thermolithobacter

species: Thermolithobacter ferrireducens

full scientific name: Thermolithobacter ferrireducens Sokolova et al. 2007

strain designation: JW/KA-2

type strain: yes

Culture and growth conditions

culture medium

• @ref: 4934
• name: THERMOLITHOBACTER MEDIUM (DSMZ Medium 778a)
• growth: yes
• link: https://mediadive.dsmz.de/medium/778a
• composition: Name: THERMOLITHOBACTER MEDIUM (DSMZ Medium 778a) Composition: Na2-9,10-anthraquinone-2,6-disulfonate 8.22532 g/l NaHCO3 2.49252 g/l Yeast extract 0.997009 g/l KH2PO4 0.329013 g/l NH4Cl 0.329013 g/l KCl 0.329013 g/l CaCl2 x 2 H2O 0.329013 g/l MgCl2 x 6 H2O 0.329013 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l NiCl2 x 6 H2O 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

• @ref: 4934
• growth: positive
• type: growth
• temperature: 65
• range: thermophilic

Physiology and metabolism

oxygen tolerance

• @ref: 4934
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 4934
• sample type: mixed sample of geothermally heated sediment and water
• geographic location: Wyoming, Yellowstone National Park, Calcite spring area
• country: USA
• origin.country: USA
• continent: North America

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_169790.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7552;96_35809;97_45485;98_111302;99_169790&stattab=map
• Last taxonomy: Thermolithobacter
• 16S sequence: AF282254
• Sequence Identity:
• Total samples: 79
• soil counts: 8
• aquatic counts: 55
• animal counts: 15
• plant counts: 1

Safety information

risk assessment

• @ref: 4934
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

GC content

• @ref: 4934
• GC-content: 52.7

External links

@ref: 4934

culture collection no.: DSM 13639, ATCC 700985

straininfo link

• @ref: 86643
• straininfo: 248052

literature

• topic: Phylogeny
• Pubmed-ID: 17021657
• title: Novel chemolithotrophic, thermophilic, anaerobic bacteria Thermolithobacter ferrireducens gen. nov., sp. nov. and Thermolithobacter carboxydivorans sp. nov.
• authors: Sokolova T, Hanel J, Onyenwoke RU, Reysenbach AL, Banta A, Geyer R, Gonzalez JM, Whitman WB, Wiegel J
• journal: Extremophiles
• DOI: 10.1007/s00792-006-0022-5
• year: 2006
• mesh: Anti-Bacterial Agents/pharmacology, Bacteria, Anaerobic/*classification/drug effects/genetics/growth & development/isolation & purification/metabolism, Base Composition, Carbon Monoxide/metabolism, *Chemoautotrophic Growth, DNA, Bacterial/analysis, Drug Resistance, Ferric Compounds/*metabolism, Ferrosoferric Oxide/metabolism, Geologic Sediments/*microbiology, Gram-Positive Asporogenous Rods/*classification/drug effects/genetics/growth & development/isolation & purification/metabolism, Hydrogen-Ion Concentration, Lipids/analysis, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, *Temperature, *Water Microbiology
• topic2: Metabolism

Reference