Strain identifier

BacDive ID: 17623

Type strain: Yes

Species: Wohlfahrtiimonas chitiniclastica

Strain Designation: S5

Strain history: <- E. M. Toth; S5

NCBI tax ID(s): 1123502 (strain), 400946 (species)

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General

@ref: 7669

BacDive-ID: 17623

DSM-Number: 18708

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, human pathogen

description: Wohlfahrtiimonas chitiniclastica S5 is an aerobe, mesophilic, Gram-negative human pathogen that was isolated from artificial culture of Wohlfahrtia magnitica .

NCBI tax id

NCBI tax idMatching level
1123502strain
400946species

strain history

  • @ref: 7669
  • history: <- E. M. Toth; S5

doi: 10.13145/bacdive17623.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Cardiobacteriales
  • family: Ignatzschineriaceae
  • genus: Wohlfahrtiimonas
  • species: Wohlfahrtiimonas chitiniclastica
  • full scientific name: Wohlfahrtiimonas chitiniclastica Tóth et al. 2008

@ref: 7669

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Gammaproteobacteria, not assigned to family

genus: Wohlfahrtiimonas

species: Wohlfahrtiimonas chitiniclastica

full scientific name: Wohlfahrtiimonas chitiniclastica Tóth et al. 2008

strain designation: S5

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32329negative1.75 µm0.75 µmrod-shapedno
69480negative99.996

pigmentation

  • @ref: 32329
  • production: no

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_18708_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_18708_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_18708_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_18708_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_18708_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

  • @ref: 7669
  • name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/535
  • composition: Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7669positivegrowth28mesophilic
32329positivegrowth28-37mesophilic
32329positiveoptimum32.5mesophilic
61233positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
32329positivegrowth5-10.5alkaliphile
32329positiveoptimum7.75

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32329aerobe
61233aerobe

spore formation

@refspore formationconfidence
32329no
69481no100
69480no99.989

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3232930089acetate+carbon source
3232916449alanine+carbon source
3232922653asparagine+carbon source
3232935391aspartate+carbon source
3232915740formate+carbon source
3232928757fructose+carbon source
3232929987glutamate+carbon source
3232917754glycerol+carbon source
3232928087glycogen+carbon source
3232924996lactate+carbon source
3232937684mannose+carbon source
3232918257ornithine+carbon source
3232928044phenylalanine+carbon source
3232917272propionate+carbon source
3232930031succinate+carbon source
3232926986threonine+carbon source
3232953426tween 80+carbon source

enzymes

@refvalueactivityec
32329catalase+1.11.1.6
32329cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
7669artificial culture of Wohlfahrtia magnitica (Diptera: Sarcophagidae)Wohlfahrtia magniticaBudapestHungaryHUNEurope
612333rd stage larvae of fly (Wohlfahrtia magnifica)Mezöfalva State FarmHungaryHUNEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Laboratory#Lab enrichment
#Host#Arthropoda#Insecta

Safety information

risk assessment

  • @ref: 7669
  • pathogenicity human: yes, in single cases
  • pathogenicity animal: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7669
  • description: Wohlfahrtiimonas chitiniclastica S5 partial 16S rRNA gene, type strain S5T
  • accession: AM397063
  • length: 1420
  • database: ena
  • NCBI tax ID: 1123502

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Wohlfahrtiimonas chitiniclastica DSM 18708GCA_000375345contigncbi1123502
66792Wohlfahrtiimonas chitiniclastica DSM 187081123502.3wgspatric1123502
66792Wohlfahrtiimonas chitiniclastica strain BM-Y400946.5wgspatric400946
66792Wohlfahrtiimonas chitiniclastica DSM 187082517487021draftimg1123502

GC content

  • @ref: 32329
  • GC-content: 44.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno88.736no
flagellatedno94.091no
gram-positiveno98.433no
anaerobicno95.392no
aerobicyes64.35yes
halophileno65.398no
spore-formingno97.778yes
thermophileno98.938no
glucose-utilno81.929no
glucose-fermentno86.167no

External links

@ref: 7669

culture collection no.: DSM 18708, CCM 7401, CCUG 57199

straininfo link

  • @ref: 86615
  • straininfo: 400799

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18398205Wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from Wohlfahrtia magnifica (Diptera: Sarcophagidae).Toth EM, Schumann P, Borsodi AK, Keki Z, Kovacs AL, Marialigeti KInt J Syst Evol Microbiol10.1099/ijs.0.65324-02008Animals, Base Composition, DNA, Bacterial/chemistry/genetics, Diptera/*microbiology, Fatty Acids/metabolism, Gammaproteobacteria/*classification/genetics/*isolation & purification/metabolism, Genes, Bacterial, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Terminology as TopicMetabolism
Genetics35873140Comparative Genomic Analysis of the Human Pathogen Wohlfahrtiimonas Chitiniclastica Provides Insight Into the Identification of Antimicrobial Resistance Genotypes and Potential Virulence Traits.Kopf A, Bunk B, Coldewey SM, Gunzer F, Riedel T, Schrottner PFront Cell Infect Microbiol10.3389/fcimb.2022.9124272022*Anti-Bacterial Agents/pharmacology/therapeutic use, *Drug Resistance, Bacterial/genetics, Gammaproteobacteria, Genomics, Genotype, Humans, Macrolides, Virulence/geneticsPathogenicity

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
7669Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18708)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18708
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32329Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2856328776041
61233Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 57199)https://www.ccug.se/strain?id=57199
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life28604660 35: 676-683 2017
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86615Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID400799.1StrainInfo: A central database for resolving microbial strain identifiers