Strain identifier

BacDive ID: 17323

Type strain: Yes

Species: Vibrio metschnikovii

Strain Designation: M 34

Strain history: CIP <- 1969, NCTC <- 1951, R.J. Evans: strain M 34 <- 1922, NCTC, Lister Inst., London, UK

NCBI tax ID(s): 675813 (strain), 28172 (species)

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General

@ref: 7930

BacDive-ID: 17323

DSM-Number: 19132

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Vibrio metschnikovii M 34 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from diseased fowl.

NCBI tax id

NCBI tax idMatching level
675813strain
28172species

strain history

@refhistory
7930<- CAIM <- LMG <- CCUG <- NCTC <- R. Evans; M34 <- NCTC <- Lister Institute
67770IAM 14406 <-- NCTC 8443 <-- R. J. Evans M34 <-- NCTC 262 <-- Lister Inst..
120926CIP <- 1969, NCTC <- 1951, R.J. Evans: strain M 34 <- 1922, NCTC, Lister Inst., London, UK

doi: 10.13145/bacdive17323.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Vibrionales
  • family: Vibrionaceae
  • genus: Vibrio
  • species: Vibrio metschnikovii
  • full scientific name: Vibrio metschnikovii Gamaleia 1888 (Approved Lists 1980)

@ref: 7930

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Vibrionales, not validated

family: Vibrionaceae

genus: Vibrio

species: Vibrio metschnikovii

full scientific name: Vibrio metschnikovii Gamaleia 1888

strain designation: M 34

type strain: yes

Morphology

cell morphology

  • @ref: 120926
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7930BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
33460Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120926CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
7930positivegrowth28mesophilic
33460positivegrowth25mesophilic
67770positivegrowth37mesophilic
120926positivegrowth15-41
120926nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120926
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
120926NaClpositivegrowth2-4 %
120926NaClnogrowth0 %
120926NaClnogrowth6 %
120926NaClnogrowth8 %
120926NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol+builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68369malate+assimilation25115
68369adipate-assimilation17128
68369decanoate-assimilation27689
68369gluconate+assimilation24265
68369maltose+assimilation17306
68369N-acetylglucosamine+assimilation59640
68369D-mannitol+assimilation16899
68369D-mannose+assimilation16024
68369L-arabinose-assimilation30849
68369D-glucose+assimilation17634
68369gelatin+hydrolysis5291
68369esculin+hydrolysis4853
68369urea-hydrolysis16199
68369arginine-hydrolysis29016
68369D-glucose-fermentation17634
68369tryptophan+energy source27897
68369nitrate-reduction17632
120926citrate-carbon source16947
120926esculin+hydrolysis4853
120926glucose+fermentation17234
120926lactose-fermentation17716
120926nitrate-reduction17632
120926nitrite-reduction16301
120926sodium thiosulfate-builds gas from132112
120926glucose+degradation17234
120926nitrate+respiration17632

antibiotic resistance

  • @ref: 120926
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleyes
12092635581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole+
12092615688acetoin+
12092617234glucose-

enzymes

@refvalueactivityec
68369cytochrome oxidase-1.9.3.1
68369gelatinase+
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
120926oxidase+
120926beta-galactosidase+3.2.1.23
120926alcohol dehydrogenase+1.1.1.1
120926gelatinase+/-
120926amylase+
120926DNase+
120926caseinase+3.4.21.50
120926catalase+1.11.1.6
120926tween esterase+
120926gamma-glutamyltransferase-2.3.2.2
120926lecithinase+
120926lipase+
120926lysine decarboxylase+4.1.1.18
120926ornithine decarboxylase-4.1.1.17
120926phenylalanine ammonia-lyase-4.3.1.24
120926protease+
120926tryptophan deaminase-
120926urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    45008C12:05.812
    45008C14:06.614
    45008C15:00.515
    45008C16:020.416
    45008C17:00.617
    45008C18:00.618
    45008C10:0 3OH0.211.423
    4500811 methyl 18:1 ω7c0.318.081
    45008C12:0 3OH2.613.455
    45008C13:0 ISO 2OH0.813.814
    45008C14:0 3OH/C16:1 ISO I315.485
    45008C16:0 anteiso0.315.717
    45008C16:1 ω7c32.415.819
    45008C16:1 ω7c alcohol0.615.386
    45008C16:1 ω9c0.615.774
    45008C17:1 ω6c0.316.862
    45008C17:1 ω8c0.616.792
    45008C18:1 ω7c /12t/9t18.617.824
    45008C18:1 ω9c0.717.769
    45008C18:2 ω6,9c/C18:0 ANTE2.517.724
    45008Unidentified0.515.271
    45008unk. 18.846/19:1 ω6c1.118.846
    45008unknown 12.4860.612.486
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120926-+++-++---++-+-+-+--

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
7930-+---++++-+++++--+---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120926+---+----+/-+++---++---+--+/---+--++---++--+/-------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120926+++++--+-++------+++-----------+++---------------+---+-----++-----------------+++-----+--+++-----+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
7930diseased fowlUnited KingdomGBREurope
45008Disease fowl
67770Diseased fowl
120926Diseased fowl

isolation source categories

  • Cat1: #Host
  • Cat2: #Birds

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
79302Risk group (German classification)
1209262Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Vibrio metschnikovii 16S rRNAD11209217ena28172
20218Vibrio metschnikovii 16S rRNAD11258214ena28172
20218Vibrio metschnikovii 16S rRNAD11307192ena28172
7930V.metschnikovii (CIP 69.14T) gene for 16S ribosomal RNAx747111443ena28172

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Vibrio metschnikovii CIP 69.14675813.3wgspatric675813
66792Vibrio metschnikovii strain NCTC844328172.6wgspatric28172
66792Vibrio metschnikovii CIP 69.14647000339draftimg675813
67770Vibrio metschnikovii CIP 69.14GCA_000176155contigncbi675813
67770Vibrio metschnikovii NCTC8443GCA_900460295contigncbi28172

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.821no
anaerobicno95.003no
halophileno51.51no
spore-formingno95.745no
glucose-utilyes93.368yes
thermophileno98.648yes
flagellatedyes85.195no
motileyes89.47no
aerobicyes86.468no
glucose-fermentyes88.501no

External links

@ref: 7930

culture collection no.: CCUG 7490, NCTC 262, ATCC 700040, CIP 69.14, JCM 21189, DSM 19132, CAIM 317, ICPB 3551, LMG 11664, NCTC 8443, GIFU 3198, IAM 14406, NBRC 103153

straininfo link

  • @ref: 86323
  • straininfo: 5099

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phenotype3182990Characterization of Vibrio metschnikovii and Vibrio gazogenes by DNA-DNA hybridization and phenotype.Farmer JJ 3rd, Hickman-Brenner FW, Fanning GR, Gordon CM, Brenner DJJ Clin Microbiol10.1128/jcm.26.10.1993-2000.19881988DNA, Bacterial/*analysis, Humans, *Nucleic Acid Hybridization, Phenotype, Vibrio/classification/*geneticsPhylogeny
Enzymology22012584Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii.Park JY, Park JE, Park JW, Yoon SM, Lee JSInt J Mol Med10.3892/ijmm.2011.8132011Cations, Divalent/metabolism, Enzyme Activation, Extracellular Space/enzymology, Fibrin/metabolism, Fibrinogen/metabolism, Hot Temperature, Kinetics, Protein Denaturation, Proteolysis, Prothrombin/metabolism, Serine Endopeptidases/chemistry/isolation & purification/*metabolism, Thrombin/metabolism, Vibrio/*enzymologyMetabolism
Phylogeny25964518Vibrio salilacus sp. nov., a new member of the Anguillarum clade with six alleles of the 16S rRNA gene from a saline lake.Zhong ZP, Liu Y, Liu HC, Wang F, Zhou YG, Liu ZPInt J Syst Evol Microbiol10.1099/ijs.0.0003162015Alleles, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Lakes/*microbiology, Molecular Sequence Data, Multilocus Sequence Typing, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Sodium Chloride/analysis, Vibrio/*classification/genetics/isolation & purificationGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7930Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19132)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19132
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33460Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10424
45008Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 7490)https://www.ccug.se/strain?id=7490
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
86323Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID5099.1StrainInfo: A central database for resolving microbial strain identifiers
120926Curators of the CIPCollection of Institut Pasteur (CIP 69.14)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2069.14