Strain identifier

BacDive ID: 17211

Type strain: Yes

Species: Luteolibacter cuticulihirudinis

Strain Designation: E100

Strain history: CIP <- 2012, P. Kämpfer, Giessen Univ., Giessen, Germany: strain E100

NCBI tax ID(s): 1157309 (species)

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General

@ref: 18115

BacDive-ID: 17211

DSM-Number: 25813

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped

description: Luteolibacter cuticulihirudinis E100 is an aerobe, Gram-negative, motile bacterium that was isolated from surface of a juvenile medicinal leech .

NCBI tax id

  • NCBI tax id: 1157309
  • Matching level: species

strain history

@refhistory
419532012, P. Kämpfer, J.L. Univ., Giessen, Germany: strain E100
18115<- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany; E100
118825CIP <- 2012, P. Kämpfer, Giessen Univ., Giessen, Germany: strain E100

doi: 10.13145/bacdive17211.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/verrucomicrobiota
  • domain: Bacteria
  • phylum: Verrucomicrobiota
  • class: Verrucomicrobiia
  • order: Verrucomicrobiales
  • family: Verrucomicrobiaceae
  • genus: Luteolibacter
  • species: Luteolibacter cuticulihirudinis
  • full scientific name: Luteolibacter cuticulihirudinis Glaeser et al. 2012

@ref: 18115

domain: Bacteria

phylum: Verrucomicrobia

class: Verrucomicrobiae

order: Verrucomicrobiales

family: Verrucomicrobiaceae

genus: Luteolibacter

species: Luteolibacter cuticulihirudinis

full scientific name: Luteolibacter cuticulihirudinis Glaeser et al. 2012 emend. Kim et al. 2015

strain designation: E100

type strain: yes

Morphology

cell morphology

  • @ref: 118825
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 18115
  • incubation period: 8-14 days

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41953MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)
18115R2A MEDIUM (DSMZ Medium 830)yesName: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled waterhttps://mediadive.dsmz.de/medium/830
118825CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperature
18115positivegrowth28
41953positivegrowth30
62832positivegrowth30

Physiology and metabolism

oxygen tolerance

  • @ref: 62832
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11882517632nitrate-reduction
11882516301nitrite-reduction

metabolite production

  • @ref: 118825
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
118825oxidase+
118825catalase+1.11.1.6
118825urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118825-+---+----++-----+--

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
18115surface of a juvenile medicinal leech ( Hirudo medicinalis )Hirudo medicinalisHessen, BiebertalGermanyDEUEurope
62832Surface of juvenile leech (Hirudo medicinalis)Biebertal (Hessen)GermanyDEUEurope
118825Animal, Surface of a juvenile leech, Hirudo verbanaBiebertal, HessenGermanyDEUEurope

isolation source categories

  • Cat1: #Host
  • Cat2: #Invertebrates (Other)
  • Cat3: #Annelida

taxonmaps

  • @ref: 69479
  • File name: preview.99_44201.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_545;96_20349;97_25307;98_32084;99_44201&stattab=map
  • Last taxonomy: Luteolibacter cuticulihirudinis subclade
  • 16S sequence: JQ429496
  • Sequence Identity:
  • Total samples: 908
  • soil counts: 362
  • aquatic counts: 195
  • animal counts: 162
  • plant counts: 189

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
181151Risk group (German classification)
1188251Risk group (French classification)

Sequence information

16S sequences

  • @ref: 18115
  • description: Luteolibacter cuticulihirudinis strain E100 16S ribosomal RNA gene, partial sequence
  • accession: JQ429496
  • length: 1393
  • database: nuccore
  • NCBI tax ID: 1157309

GC content

  • @ref: 18115
  • GC-content: 47.2
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 18115

culture collection no.: DSM 25813, CCM 8400, CCUG 62419, LMG 26924, CIP 110383

straininfo link

  • @ref: 86210
  • straininfo: 379503

literature

  • topic: Phylogeny
  • Pubmed-ID: 22552629
  • title: Luteolibacter cuticulihirudinis sp. nov., isolated from Hirudo medicinalis.
  • authors: Glaeser SP, Galatis H, Martin K, Kampfer P
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-012-9741-z
  • year: 2012
  • mesh: Animals, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Hirudo medicinalis/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Verrucomicrobia/classification/genetics/*isolation & purification
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
18115Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25813)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25813
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41953Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8237
62832Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 62419)https://www.ccug.se/strain?id=62419
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86210Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID379503.1StrainInfo: A central database for resolving microbial strain identifiers
118825Curators of the CIPCollection of Institut Pasteur (CIP 110383)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110383