Strain identifier
BacDive ID: 17074
Type strain: 
Species: Defluviitoga tunisiensis
Strain Designation: Rsulflac1, Sulflac1
Strain history: W. Ben Hania et al. strain SulfLac1.
NCBI tax ID(s): 1006576 (species)
version 8.1 (current version)
General
@ref: 17375
BacDive-ID: 17074
DSM-Number: 23805
keywords: 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative, rod-shaped
description: Defluviitoga tunisiensis Rsulflac1 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from anaerobic whey digester.
NCBI tax id
- NCBI tax id: 1006576
- Matching level: species
strain history
| @ref | history |
|---|---|
| 17375 | <- M. L. Fardeau, Lab. Microbiol. de l'IRD/ESIL, Univ. de Provence et de la Méditerranée, Marseille, France; Sulflac1 <- W. Ben Hania {2008} |
| 67770 | W. Ben Hania et al. strain SulfLac1. |
doi: 10.13145/bacdive17074.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/thermotogota
- domain: Bacteria
- phylum: Thermotogota
- class: Thermotogae
- order: Petrotogales
- family: Petrotogaceae
- genus: Defluviitoga
- species: Defluviitoga tunisiensis
- full scientific name: Defluviitoga tunisiensis Ben Hania et al. 2012
@ref: 17375
domain: Bacteria
phylum: Thermotogae
class: Thermotogae
order: Thermotogales
family: Petrotogaceae
genus: Defluviitoga
species: Defluviitoga tunisiensis
full scientific name: Defluviitoga tunisiensis Ben Hania et al. 2012
strain designation: Rsulflac1, Sulflac1
type strain: yes
Morphology
cell morphology
- @ref: 30336
- gram stain: negative
- cell length: 15 µm
- cell width: 1 µm
- cell shape: rod-shaped
colony morphology
- @ref: 17375
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 17375
- name: DEFLUVIITOGA MEDIUM (DSMZ Medium 1328)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1328
- composition: Name: DEFLUVIITOGA MEDIUM (DSMZ Medium 1328) Composition: Sulfur 10.0 g/l Na2-fumarate 3.2 g/l Trypticase peptone 2.0 g/l Yeast extract 1.0 g/l Na2CO3 1.0 g/l NaCl 1.0 g/l NH4Cl 1.0 g/l Na2S x 9 H2O 0.5 g/l L-Cysteine HCl x H2O 0.5 g/l MgCl2 x 6 H2O 0.5 g/l KH2PO4 0.3 g/l K2HPO4 0.3 g/l KCl 0.1 g/l CaCl2 x 2 H2O 0.1 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 17375 | positive | growth | 55 | thermophilic |
| 30336 | positive | growth | 37-65 | |
| 30336 | positive | optimum | 55 | thermophilic |
| 67770 | positive | growth | 55 | thermophilic |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 30336 | positive | growth | 6.5-7.9 |
| 30336 | positive | optimum | 6.9 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 17375 | anaerobe |
| 30336 | anaerobe |
spore formation
- @ref: 30336
- spore formation: no
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 30336 | NaCl | positive | growth | 0.2-3 % |
| 30336 | NaCl | positive | optimum | 0.50 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30336 | 37684 | mannose | + | carbon source |
| 30336 | 16634 | raffinose | + | carbon source |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 17375 | anaerobic whey digester | Tunis | Tunisia | TUN | Africa |
| 67770 | Anaerobic reactor used to digest whey in Tunisia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Biodegradation | #Anaerobic digestor |
| #Engineered | #Food production | #Dairy product |
| #Condition | #Anoxic (anaerobic) |
taxonmaps
- @ref: 69479
- File name: preview.99_8139.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_617;96_3754;97_4616;98_5925;99_8139&stattab=map
- Last taxonomy: Defluviitoga tunisiensis subclade
- 16S sequence: FR850164
- Sequence Identity:
- Total samples: 6129
- soil counts: 863
- aquatic counts: 3470
- animal counts: 1397
- plant counts: 399
Safety information
risk assessment
- @ref: 17375
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17375
- description: Defluviitoga tunisiensis partial 16S rRNA gene, strain SulfLac1
- accession: FR850164
- length: 1475
- database: ena
- NCBI tax ID: 1006576
GC content
| @ref | GC-content | method |
|---|---|---|
| 17375 | 33.6 | high performance liquid chromatography (HPLC) |
| 30336 | 33.6 |
External links
@ref: 17375
culture collection no.: DSM 23805, JCM 17210
straininfo link
- @ref: 86074
- straininfo: 406121
literature
- topic: Phylogeny
- Pubmed-ID: 21828011
- title: Defluviitoga tunisiensis gen. nov., sp. nov., a thermophilic bacterium isolated from a mesothermic and anaerobic whey digester.
- authors: Ben Hania W, Godbane R, Postec A, Hamdi M, Ollivier B, Fardeau ML
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.033720-0
- year: 2011
- mesh: Anaerobiosis, Bacteria/*classification/genetics/*isolation & purification, Bioreactors/*microbiology, DNA, Bacterial/genetics, Hot Temperature, Milk Proteins/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sulfates/metabolism, Tunisia, Whey Proteins
- topic2: Stress
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 17375 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23805) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23805 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 30336 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26677 | 28776041 | ||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 86074 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID406121.1 | StrainInfo: A central database for resolving microbial strain identifiers |