General

@ref: 5165

BacDive-ID: 17053

DSM-Number: 13782

keywords: 16S sequence, Bacteria, anaerobe, thermophilic

description: Thermosipho africanus TB5A is an anaerobe, thermophilic bacterium that was isolated from production water from an oil well.

NCBI tax id

• NCBI tax id: 2421
• Matching level: species

strain history

• @ref: 5165
• history: <- T. Lien, Univ. Bergen, Norway; TB5A (Thermosipho sp.) <- O. C. Bjørgo {1999}

doi: 10.13145/bacdive17053.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/thermotogota
• domain: Bacteria
• phylum: Thermotogota
• class: Thermotogae
• order: Thermotogales
• family: Fervidobacteriaceae
• genus: Thermosipho
• species: Thermosipho africanus
• full scientific name: Thermosipho africanus Huber et al. 1989

@ref: 5165

domain: Bacteria

phylum: Thermotogae

class: Thermotogae

order: Thermotogales

family: Fervidobacteriaceae

genus: Thermosipho

species: Thermosipho africanus

full scientific name: Thermosipho africanus Huber et al. 1989 emend. Ravot et al. 1996

strain designation: TB5A

type strain: no

Culture and growth conditions

culture medium

• @ref: 5165
• name: PETROTOGA MEDIUM (DSMZ Medium 718)
• growth: yes
• link: https://mediadive.dsmz.de/medium/718
• composition: Name: PETROTOGA MEDIUM (DSMZ Medium 718; with strain-specific modifications) Composition: Na2S2O3 x 5 H2O 4.93583 g/l D-Glucose 4.93583 g/l MgCl2 x 6 H2O 3.94867 g/l Trypticase peptone 0.987167 g/l Yeast extract 0.987167 g/l NaHCO3 0.987167 g/l Na2S x 9 H2O 0.493583 g/l KCl 0.335637 g/l NH4Cl 0.246792 g/l K2HPO4 0.138203 g/l MgSO4 x 7 H2O 0.029615 g/l Nitrilotriacetic acid 0.0148075 g/l NaCl 0.00987167 g/l MnSO4 x H2O 0.00493583 g/l Fe(NH4)2(SO4)2 x 7 H2O 0.00197433 g/l CoSO4 x 7 H2O 0.0017769 g/l ZnSO4 x 7 H2O 0.0017769 g/l CaCl2 x 2 H2O 0.000987167 g/l FeSO4 x 7 H2O 0.000987167 g/l Sodium resazurin 0.000493583 g/l NiCl2 x 6 H2O 0.00029615 g/l AlK(SO4)2 x 12 H2O 0.000197433 g/l CuSO4 x 5 H2O 9.87167e-05 g/l Na2MoO4 x 2 H2O 9.87167e-05 g/l Pyridoxine hydrochloride 9.87167e-05 g/l H3BO3 9.87167e-05 g/l (DL)-alpha-Lipoic acid 4.93583e-05 g/l Calcium D-(+)-pantothenate 4.93583e-05 g/l Nicotinic acid 4.93583e-05 g/l p-Aminobenzoic acid 4.93583e-05 g/l Riboflavin 4.93583e-05 g/l Thiamine HCl 4.93583e-05 g/l Folic acid 1.97433e-05 g/l Biotin 1.97433e-05 g/l Na2WO4 x 2 H2O 3.94867e-06 g/l Na2SeO3 x 5 H2O 2.9615e-06 g/l Vitamin B12 9.87167e-07 g/l Distilled water

culture temp

• @ref: 5165
• growth: positive
• type: growth
• temperature: 60
• range: thermophilic

Physiology and metabolism

oxygen tolerance

• @ref: 5165
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 5165
• sample type: production water from an oil well

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_547.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_93;96_347;97_387;98_444;99_547&stattab=map
• Last taxonomy: Thermosipho
• 16S sequence: AF231727
• Sequence Identity:
• Total samples: 46
• aquatic counts: 33
• animal counts: 12
• plant counts: 1

Safety information

risk assessment

• @ref: 5165
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 5165
• description: Thermosipho sp. TBA5 16S ribosomal RNA gene, partial sequence
• accession: AF231727
• length: 1567
• database: ena
• NCBI tax ID: 114811

External links

@ref: 5165

culture collection no.: DSM 13782

straininfo link

• @ref: 86053
• straininfo: 407940

Reference