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Strain identifier

BacDive ID: 17017

Type strain: Yes

Species: Thermoplasma acidophilum

Strain Designation: 122-1B2

Strain history: IFO 15155 <-- T. Itoh <-- ATCC 25905 <-- R. G. Wittler <-- T. D. Brock <-- G. Darland 122-1B2.

NCBI tax ID(s): 273075 (strain), 2303 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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version 7.1 (current version)


@ref: 884

BacDive-ID: 17017

DSM-Number: 1728

keywords: genome sequence, 16S sequence, Archaea, thermophilic

description: Thermoplasma acidophilum 122-1B2 is a thermophilic archaeon that was isolated from coal refuse pile.

NCBI tax id

NCBI tax idMatching level

strain history

doi: 10.13145/bacdive17017.20221219.7.1

Name and taxonomic classification


  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/euryarchaeota
  • domain: Archaea
  • phylum: Euryarchaeota
  • class: Thermoplasmata
  • order: Thermoplasmatales
  • family: Thermoplasmataceae
  • genus: Thermoplasma
  • species: Thermoplasma acidophilum
  • full scientific name: Thermoplasma acidophilum corrig. Darland et al. 1970 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Thermoplasma acidophila

@ref: 884

domain: Archaea

phylum: Euryarchaeota

class: Thermoplasmata

order: Thermoplasmatales

family: Thermoplasmataceae

genus: Thermoplasma

species: Thermoplasma acidophilum

full scientific name: Thermoplasma acidophilum Darland et al. 1970

strain designation: 122-1B2

type strain: yes


cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.178

Culture and growth conditions

culture medium

  • @ref: 884
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/158
  • composition: Name: THERMOPLASMA ACIDOPHILUM MEDIUM (DSMZ Medium 158) Composition: Glucose 20.0 g/l Yeast extract 2.0 g/l (NH4)2SO4 1.32 g/l KH2PO4 0.372 g/l MgSO4 x 7 H2O 0.247 g/l CaCl2 x 2 H2O 0.074 g/l FeCl3 x 6 H2O 0.0193 g/l Na2B4O7 x 10 H2O 0.0045 g/l MnCl2 x 4 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.00022 g/l CuCl2 x 2 H2O 5e-05 g/l VOSO4 x 5 H2O 3.8e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l CoSO4 x 7 H2O 2e-05 g/l Distilled water

culture temp


Physiology and metabolism

oxygen tolerance

  • @ref: 69480
  • oxygen tolerance: anaerobe
  • confidence: 75.61

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 97.807

compound production

  • @ref: 884
  • compound: restriction endonuclease ThaI


  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 71.379


  • @ref: 67770
  • observation: quinones: MK-7, MMK-7, MK-7, MMK-7

Isolation, sampling and environmental information


@refsample typecountryorigin.countrycontinent
884coal refuse pileUSAUSANorth America
67770Coal refuse pile

isolation source categories

#Engineered#Waste#Solid waste


  • @ref: 69479
  • File name: preview.99_187333.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17823;96_68848;97_89494;98_121774;99_187333&stattab=map
  • Last taxonomy: Thermoplasma
  • 16S sequence: M38637
  • Sequence Identity:
  • Total samples: 38
  • aquatic counts: 38

Safety information

risk assessment

  • @ref: 884
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
884Thermoplasma acidophilum 16S rRNA geneM386372914ena2303
884Citrus unshiu DNA, scaffold00261, cultivar: Miyagawa wase, whole genome shotgun sequenceM20822321284ena55188

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thermoplasma acidophilum DSM 1728 DSM1728GCA_000195915completencbi273075
66792Thermoplasma acidophilum DSM 1728638154521completeimg273075
67770Thermoplasma acidophilum DSM 1728 complete genomeAL139299ena273075

GC content

6777045high performance liquid chromatography (HPLC)
6777045Buoyant density centrifugation (BD)
6777046.9Buoyant density centrifugation (BD)
6777046thermal denaturation, midpoint method (Tm)

External links

@ref: 884

culture collection no.: DSM 1728, ATCC 25905, AMRC C165, JCM 9062, IFO 15155, NBRC 15155

straininfo link



Metabolism17827289Structural and mutational analysis of tRNA intron-splicing endonuclease from Thermoplasma acidophilum DSM 1728: catalytic mechanism of tRNA intron-splicing endonucleases.Kim YK, Mizutani K, Rhee KH, Nam KH, Lee WH, Lee EH, Kim EE, Park SY, Hwang KYJ Bacteriol10.1128/JB.00713-072007Amino Acid Sequence, Archaeal Proteins/chemistry/genetics/metabolism, Catalysis, Endonucleases/genetics/*metabolism, Introns/*genetics, Models, Molecular, Mutation/*genetics, Protein Conformation, *RNA Splicing, RNA, Bacterial/genetics, RNA, Transfer/*genetics, Thermoplasma/classification/*enzymology/*geneticsEnzymology
Metabolism18309272Crystallization and preliminary X-ray diffraction analysis of 5,10- methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum DSM 1728.Kim JH, Sung MW, Lee EH, Nam KH, Hwang KYJ Microbiol Biotechnol71372008Aminohydrolases/*chemistry/metabolism, Archaeal Proteins/*chemistry/metabolism, Crystallization, Crystallography, X-Ray, Escherichia coli/genetics/metabolism, Methylenetetrahydrofolate Dehydrogenase (NADP)/*chemistry/metabolism, Multienzyme Complexes/*chemistry/metabolism, Thermoplasma/chemistry/*enzymologyEnzymology
Enzymology19058034Heterologous expression and characterization of an alcohol dehydrogenase from the archeon Thermoplasma acidophilum.Marino-Marmolejo EN, De Leon-Rodriguez A, de la Rosa AP, Santos LMol Biotechnol10.1007/s12033-008-9130-z2008Alcohol Dehydrogenase/*chemistry/genetics/*metabolism, Alcohols/metabolism, Amino Acid Sequence, Escherichia coli/genetics, Hydrogen-Ion Concentration, Kinetics, Molecular Sequence Data, Recombinant Proteins/*chemistry/genetics/*metabolism, Sequence Analysis, DNA, Substrate Specificity, Temperature, Thermoplasma/*enzymologyMetabolism
Metabolism31640225The Main (Glyco) Phospholipid (MPL) of Thermoplasma acidophilum.Freisleben HJInt J Mol Sci10.3390/ijms202052172019Archaeal Proteins/chemistry/metabolism, Calorimetry, Differential Scanning, Computer Simulation, Differential Thermal Analysis, Glycosylation, Liposomes/metabolism, Phospholipids/chemistry/*metabolism, Thermoplasma/*metabolism


884Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1728)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1728
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)