Strain identifier

BacDive ID: 170

Type strain: Yes

Species: Schaalia hyovaginalis

Strain Designation: BM 1192/5

Strain history: CIP <- 1993, NCFB <- Collins, Reading, UK: strain BM 1192/5 <- L. Devriese, Ghent Univ., Ghent, Belgium

NCBI tax ID(s): 29316 (species)

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General

@ref: 4040

BacDive-ID: 170

DSM-Number: 10695

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, animal pathogen

description: Schaalia hyovaginalis BM 1192/5 is an anaerobe, mesophilic, Gram-positive animal pathogen that was isolated from Purulent Lesions in Pigs.

NCBI tax id

  • NCBI tax id: 29316
  • Matching level: species

strain history

@refhistory
4040<- NCFB <- L.A. Devriese, BM 1192/5
120031CIP <- 1993, NCFB <- Collins, Reading, UK: strain BM 1192/5 <- L. Devriese, Ghent Univ., Ghent, Belgium

doi: 10.13145/bacdive170.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Schaalia
  • species: Schaalia hyovaginalis
  • full scientific name: Schaalia hyovaginalis (Collins et al. 1993) Nouioui et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Actinomyces hyovaginalis

@ref: 4040

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Schaalia

species: Schaalia hyovaginalis

full scientific name: Schaalia hyovaginalis (Collins et al. 1993) Nouioui et al. 2018

strain designation: BM 1192/5

type strain: yes

Morphology

cell morphology

  • @ref: 120031
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 120031
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4040FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203)yeshttps://mediadive.dsmz.de/medium/1203Name: FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) Composition: Horse blood 100.0 g/l Fastidious Anaerobe Agar 45.7 g/l Distilled water
4040COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
40874MEDIUM 39 - for ActinomycesyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
120031CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
4040positivegrowth37mesophilic
40874positivegrowth37mesophilic
52851positivegrowth37mesophilic
120031positivegrowth25-41
120031nogrowth10psychrophilic
120031nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4040anaerobe
52851anaerobe
52851microaerophile

halophily

@refsaltgrowthtested relationconcentration
120031NaClpositivegrowth0-2 %
120031NaClnogrowth4 %
120031NaClnogrowth6 %
120031NaClnogrowth8 %
120031NaClnogrowth10 %

murein

  • @ref: 4040
  • murein short key: A21.11
  • type: A5ß L-Orn-L-Lys-D-Glu

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120031citrate-carbon source16947
120031esculin+hydrolysis4853
120031hippurate+hydrolysis606565
120031nitrate+reduction17632
120031nitrite-reduction16301
68380urea-hydrolysis16199
68380arginine-hydrolysis29016
68380D-mannose-fermentation16024
68380raffinose-fermentation16634
68380L-glutamate-degradation29985
68380nitrate-reduction17632
68380tryptophan-energy source27897

antibiotic resistance

  • @ref: 120031
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
12003135581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6838035581indole-
12003115688acetoin-
12003117234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380tryptophan deaminase-4.1.99.1
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
120031oxidase-
120031beta-galactosidase+3.2.1.23
120031alcohol dehydrogenase-1.1.1.1
120031gelatinase-
120031amylase-
120031DNase+
120031caseinase-3.4.21.50
120031catalase-1.11.1.6
120031tween esterase+/-
120031gamma-glutamyltransferase-2.3.2.2
120031lecithinase+
120031lysine decarboxylase-4.1.1.18
120031ornithine decarboxylase-4.1.1.17
120031phenylalanine ammonia-lyase-4.3.1.24
120031tryptophan deaminase-
120031urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    52851C12:00.912
    52851C14:02.914
    52851C16:023.516
    52851C18:013.718
    52851C16:1 ω7c1.315.819
    52851C16:1 ω9c1.415.774
    52851C17:1 ISO I/C16:0 DMA1.416.481
    52851C18:1 ω9c21.417.769
    52851C18:2 ω6,9c/C18:0 ANTE25.817.724
    52851C19:0 ISO1.218.633
    52851Unidentified6.718.195
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120031-----+----+---------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120031----------+++------------------------------------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
4040------+/-------+/---+/-+/-+-++-+--+-+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
23116Purulent Lesions in Pigs
52851Pig vaginaGentBelgiumBELEurope
4040pig
120031Animal, Porcine, genital tractBelgiumBELEurope

isolation source categories

Cat1Cat2Cat3
#Infection#Inflammation
#Host#Mammals#Suidae (Pig,Swine)
#Host Body-Site#Other#Wound
#Host Body Product#Fluids

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
4040yes2Risk group (German classification)
1200312Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4040
  • description: A.hyovaginalis gene for 16S rRNA
  • accession: X69616
  • length: 1496
  • database: ena
  • NCBI tax ID: 29316

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Schaalia hyovaginalis DSM 10695GCA_014208035contigncbi29316
66792Schaalia hyovaginalis strain DSM 1069529316.4wgspatric29316
66792Schaalia hyovaginalis DSM 106952870625619draftimg29316

GC content

@refGC-contentmethod
404063thermal denaturation, midpoint method (Tm)
2311663.0Thermal denaturation, fluorometry

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes96.112no
anaerobicyes62.05no
halophileno88.87no
spore-formingno95.889no
thermophileno94.424no
glucose-utilyes88.635no
motileno92.218no
flagellatedno96.329no
aerobicno93.959yes
glucose-fermentyes85.5no

External links

@ref: 4040

culture collection no.: DSM 10695, ATCC 51367, CIP 103923, NCFB 2983, CCUG 35604, CCUG 35715

straininfo link

  • @ref: 69852
  • straininfo: 42952

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4040Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10695)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10695
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23116M. D. Collins, S. Stubbs, J. Hommez, L. A. Devriese10.1099/00207713-43-3-471Molecular Taxonomic Studies of Actinomyces-Like Bacteria Isolated from Purulent Lesions in Pigs and Description of Actinomyces hyovaginalis sp. nov.IJSEM 43: 471-473 19938347506
40874Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15774
52851Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 35604)https://www.ccug.se/strain?id=35604
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69852Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42952.1StrainInfo: A central database for resolving microbial strain identifiers
120031Curators of the CIPCollection of Institut Pasteur (CIP 103923)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103923