Strain identifier

BacDive ID: 169287

Type strain: No

Species: Rhodococcus sp.

Strain Designation: 3HYL

Strain history: <- M. El Hassouni, University Sidi Mohamed Ben Abdellah, Faculté des Sciences Dhar Mahraz; 3HYL <- J. Anissi, University Sidi Mohamed Ben Abdellah, Faculté des Sciences Dhar Mahraz; 3HYL

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General

@ref: 68540

BacDive-ID: 169287

DSM-Number: 109178

keywords: 16S sequence, mesophilic

description: Rhodococcus sp. 3HYL is a mesophilic prokaryote that was isolated from Soil of an olive tree.

strain history

  • @ref: 68540
  • history: <- M. El Hassouni, University Sidi Mohamed Ben Abdellah, Faculté des Sciences Dhar Mahraz; 3HYL <- J. Anissi, University Sidi Mohamed Ben Abdellah, Faculté des Sciences Dhar Mahraz; 3HYL

doi: 10.13145/bacdive169287.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • genus: Rhodococcus
  • species: Rhodococcus sp.
  • full scientific name: Rhodococcus Zopf 1891 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Prescottella
    20215Rhodococcoides
    20215Spelaeibacter
    20215Prescottia

@ref: 68540

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Not assigned to order

family: Nocardiaceae

genus: Rhodococcus

species: Rhodococcus sp.

full scientific name: Rhodococcus sp.

strain designation: 3HYL

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlink
68540TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium535.pdf
68540GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
68540CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium220.pdf

culture temp

  • @ref: 68540
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
68540---+/--+-+/--------+/-+---

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
68540+-------++/-+/-+--+/--+++-

Isolation, sampling and environmental information

isolation

  • @ref: 68540
  • sample type: Soil of an olive tree
  • geographic location: Louata, Sefrou, Morocco (33° 54' 6.6'' N, 4° 39' 45.7'' W)
  • country: Morocco
  • origin.country: MAR
  • continent: Africa
  • latitude: 33.9018
  • longitude: -4.6627

Safety information

risk assessment

  • @ref: 68540
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 68540
  • description: Rhodococcus sp. 3HYL 16S ribosomal RNA gene, partial sequence
  • accession: FJ887962
  • length: 1420
  • database: ena
  • NCBI tax ID: 639203

External links

@ref: 68540

culture collection no.: DSM 109178

straininfo link

  • @ref: 115526
  • straininfo: 408263

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
68540Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-109178Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 109178)
115526Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID408263.1