Strain identifier

BacDive ID: 16921

Type strain: Yes

Species: Thermomonospora curvata

Strain Designation: B9, B9, A-0096

Strain history: CIP <- 1998, JCM <- KCC: strain A-0096 <- A. Henssen: strain B9

NCBI tax ID(s): 471852 (strain), 2020 (species)

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General

@ref: 10850

BacDive-ID: 16921

DSM-Number: 43183

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, thermophilic

description: Thermomonospora curvata B9 is an obligate aerobe, spore-forming, thermophilic bacterium that was isolated from straw.

NCBI tax id

NCBI tax idMatching level
471852strain
2020species

strain history

@refhistory
10850<- JCM <- A. Henssen; B9
67770KCC A-0096 <-- A. Henssen B9.
118821CIP <- 1998, JCM <- KCC: strain A-0096 <- A. Henssen: strain B9

doi: 10.13145/bacdive16921.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Thermomonosporaceae
  • genus: Thermomonospora
  • species: Thermomonospora curvata
  • full scientific name: Thermomonospora curvata Henssen 1957 (Approved Lists 1980)

@ref: 10850

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Thermomonosporaceae

genus: Thermomonospora

species: Thermomonospora curvata

full scientific name: Thermomonospora curvata Henssen 1957 emend. Nouioui et al. 2018

strain designation: B9, B9, A-0096

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no91.388
69480100positive
118821nopositiverod-shaped

multimedia

@refmultimedia contentintellectual property rightscaption
10850https://www.dsmz.de/microorganisms/photos/DSM_43183-1.jpg© Leibniz-Institut DSMZ
10850https://www.dsmz.de/microorganisms/photos/DSM_43183.jpg© Leibniz-Institut DSMZMedium 252 45°C
66793EM_DSM_43183_1.jpg© HZI/Manfred Rohdeelectron microscopic image

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10850STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
10850CYC-MEDIUM (modified following Cross and Attwell,1973) (DSMZ Medium 550)yeshttps://mediadive.dsmz.de/medium/550Name: CYC-MEDIUM (modified following Cross and Attwell,1973) (DSMZ Medium 550) Composition: Czapek Dox agar 48.0 g/l Agar 20.0 g/l Casamino acids 6.1 g/l Yeast extract 2.0 g/l Tryptophan 0.02 g/l Distilled water
34063MEDIUM 280 - for Actinomadura longicatenayesDistilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (0.200 g);Potassium nitrate (0.200 g);Di Potassium monohydrogenophosphate (0.500 g);Oats (3.000 g)
118821CIP Medium 280yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=280

culture temp

@refgrowthtypetemperaturerange
10850positivegrowth45thermophilic
34063positivegrowth50thermophilic
67770positivegrowth50thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 118821
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes99.999

halophily

@refsaltgrowthtested relationconcentration
118821NaClpositivegrowth0-2 %
118821NaClnogrowth4 %
118821NaClnogrowth6 %
118821NaClnogrowth8 %
118821NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1188214853esculin-hydrolysis
11882117632nitrate-reduction
11882116301nitrite-reduction

metabolite production

  • @ref: 118821
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 118821
  • Chebi-ID: 17234
  • metabolite: glucose
  • methylred-test: -

enzymes

@refvalueactivityec
118821oxidase-
118821beta-galactosidase+3.2.1.23
118821catalase+1.11.1.6
118821gamma-glutamyltransferase-2.3.2.2
118821tryptophan deaminase-
118821urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118821--++-+----++---+----

Isolation, sampling and environmental information

isolation

@refsample type
10850straw
67770Straw
118821Straw

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Plant#Straw
#Host#Plants#Herbaceous plants (Grass,Crops)

taxonmaps

  • @ref: 69479
  • File name: preview.99_6008.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_239;96_2866;97_3523;98_4458;99_6008&stattab=map
  • Last taxonomy: Thermomonospora curvata subclade
  • 16S sequence: X97893
  • Sequence Identity:
  • Total samples: 3035
  • soil counts: 1626
  • aquatic counts: 274
  • animal counts: 537
  • plant counts: 598

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
108501Risk group (German classification)
1188211Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Thermomonospora curvata gene for 16S rRNA, partial sequence, strain: JCM 3096AB5886311469ena471852
20218Thermomonospora curvata 16S ribosomal RNA gene, partial sequenceAF0022621464ena471852
20218Thermomonospora curvata 16S-23S ribosomal RNA intergenic spacer region, partial sequenceAF134112466ena471852
20218T.curvata 16S rRNA geneX978931459ena471852
67770Thermomonospora curvata gene for 16S ribosomal RNA, partial sequenceD869451478ena2020

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thermomonospora curvata DSM 43183GCA_000024385completencbi471852
66792Thermomonospora curvata DSM 43183471852.6completepatric471852
66792Thermomonospora curvata DSM 43183646311963completeimg471852

GC content

  • @ref: 67770
  • GC-content: 71.64
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno93.551no
flagellatedno98.589no
gram-positiveyes89.45no
anaerobicno96.539no
aerobicyes81.81no
halophileno89.271no
spore-formingyes87.742no
thermophileno69.392no
glucose-utilyes84.83no
glucose-fermentno91.797no

External links

@ref: 10850

culture collection no.: DSM 43183, ATCC 19995, CBS 141.67, IAM 14296, IMET 9551, JCM 3096, KCC A-0096, NCIMB 10081, CECT 3319, CIP 105592, HAMBI 1549, IFO 15933, IMSNU 22171, KACC 20052, KCTC 9072, NBIMCC 3643, NBRC 15933

straininfo link

  • @ref: 85926
  • straininfo: 92878

literature

Pubmed-IDtitleauthorsjournalDOIyeartopicmeshtopic2
25405080Functional characterization and structural modeling of synthetic polyester-degrading hydrolases from Thermomonospora curvata.Wei R, Oeser T, Then J, Kuhn N, Barth M, Schmidt J, Zimmermann WAMB Express10.1186/s13568-014-0044-92014
30856245Draft genome of Thermomonospora sp. CIT 1 (Thermomonosporaceae) and in silico evidence of its functional role in filter cake biomass deconstruction.Omori WP, Pinheiro DG, Kishi LT, Fernandes CC, Fernandes GC, Gomes-Pepe ES, Pavani CD, Lemos EGM, Souza JAMGenet Mol Biol10.1590/1678-4685-GMB-2017-03762019
31180315Thermomonospora catenispora sp. nov., isolated from mushroom compost.Wu H, Wei J, Liu BInt J Syst Evol Microbiol10.1099/ijsem.0.0035152019PhylogenyActinomycetales/*classification/isolation & purification, *Agaricales, Bacterial Typing Techniques, Base Composition, China, *Composting, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycolipids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
34399224A new thermophilic extradiol dioxygenase promises biodegradation of catecholic pollutants.Zhang X, Huang Z, Wang D, Zhang Y, Eser BE, Gu Z, Dai R, Gao R, Guo ZJ Hazard Mater10.1016/j.jhazmat.2021.1268602021MetabolismCatechols, *Dioxygenases/genetics, *Environmental Pollutants, Molecular Docking Simulation, Oxygenases/genetics/metabolism, Phylogeny, Substrate SpecificityPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10850Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43183)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43183
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34063Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17628
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85926Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92878.1StrainInfo: A central database for resolving microbial strain identifiers
118821Curators of the CIPCollection of Institut Pasteur (CIP 105592)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105592