Strain identifier

BacDive ID: 16876

Type strain: Yes

Species: Thermococcus aegaeus

Strain Designation: BK17S6-3-b2, P5

Strain history: M. Thomm P5.

NCBI tax ID(s): 84597 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4809

BacDive-ID: 16876

DSM-Number: 12767

keywords: 16S sequence, Archaea, anaerobe, hyperthermophilic

description: Thermococcus aegaeus BK17S6-3-b2 is an anaerobe, hyperthermophilic archaeon that was isolated from marine hydrothermal vent.

NCBI tax id

  • NCBI tax id: 84597
  • Matching level: species

strain history

@refhistory
4809<- M. Thomm; P5
67770M. Thomm P5.

doi: 10.13145/bacdive16876.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Thermococci
  • order: Thermococcales
  • family: Thermococcaceae
  • genus: Thermococcus
  • species: Thermococcus aegaeus
  • full scientific name: Thermococcus aegaeus corrig. Arab et al. 2000
  • synonyms

    • @ref: 20215
    • synonym: Thermococcus aegaeicus

@ref: 4809

domain: Archaea

phylum: Euryarchaeota

class: Thermococci

order: Thermococcales

family: Thermococcaceae

genus: Thermococcus

species: Thermococcus aegaeus

full scientific name: Thermococcus aegaeus Arab et al. 2000

strain designation: BK17S6-3-b2, P5

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 4809
  • name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/760
  • composition: Name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
4809positivegrowth88-90hyperthermophilic
67770positivegrowth90hyperthermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 4809
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4809marine hydrothermal ventAegean See, Palaeochori Bay, MilosGreeceGRCEurope
67770Sediment of Palaeochori BayMilosGreeceGRCEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Hydrothermal vent
#Environmental#Aquatic#Marine
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_188403.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17827;96_69204;97_89896;98_122226;99_188403&stattab=map
  • Last taxonomy: Thermococcus
  • 16S sequence: AY099171
  • Sequence Identity:
  • Total samples: 35
  • soil counts: 1
  • aquatic counts: 34

Safety information

risk assessment

  • @ref: 4809
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Thermococcus aegaeus strain DSM 12767 16S ribosomal RNA gene, partial sequenceAY0991711453ena84597
20218Thermococcus aegaeus strain DSM 12767 16S-23S ribosomal RNA intergenic spacer region, complete sequenceAY099207185ena84597
20218Thermococcus aegeaicus (isolate P5) 16S rRNA gene, partialAJ0126431325ena84597

GC content

@refGC-contentmethod
480945
6777045.5high performance liquid chromatography (HPLC)

External links

@ref: 4809

culture collection no.: DSM 12767, JCM 10828

straininfo link

  • @ref: 85884
  • straininfo: 101004

literature

  • topic: Phylogeny
  • Pubmed-ID: 11155985
  • title: Thermococcus aegaeicus sp. nov. and Staphylothermus hellenicus sp. nov., two novel hyperthermophilic archaea isolated from geothermally heated vents off Palaeochori Bay, Milos, Greece.
  • authors: Arab H, Volker H, Thomm M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-50-6-2101
  • year: 2000
  • mesh: Base Composition, DNA, Ribosomal/analysis, Desulfurococcaceae/*classification/genetics/growth & development/isolation & purification, Greece, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, *Temperature, Thermococcus/*classification/genetics/growth & development/isolation & purification
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4809Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12767)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12767
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85884Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID101004.1StrainInfo: A central database for resolving microbial strain identifiers