Strain identifier
BacDive ID: 16844
Type strain: 
Species: Thermoanaerobacter siderophilus
Strain Designation: SR4
Strain history: <- A. Slobodkin, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; SR4 <- A. Slobodkin {1998}
NCBI tax ID(s): 106578 (species)
version 8.1 (current version)
General
@ref: 4691
BacDive-ID: 16844
DSM-Number: 12299
keywords: genome sequence, Bacteria, anaerobe, thermophilic
description: Thermoanaerobacter siderophilus SR4 is an anaerobe, thermophilic bacterium that was isolated from hydrothermal vent.
NCBI tax id
- NCBI tax id: 106578
- Matching level: species
strain history
- @ref: 4691
- history: <- A. Slobodkin, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; SR4 <- A. Slobodkin {1998}
doi: 10.13145/bacdive16844.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Thermoanaerobacter
- species: Thermoanaerobacter siderophilus
- full scientific name: Thermoanaerobacter siderophilus Slobodkin et al. 1999
@ref: 4691
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Thermoanaerobacter
species: Thermoanaerobacter siderophilus
full scientific name: Thermoanaerobacter siderophilus Slobodkin et al. 1999
strain designation: SR4
type strain: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 4691 | THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144) | yes | https://mediadive.dsmz.de/medium/144 | Name: THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144; with strain-specific modifications) Composition: Trypticase peptone 10.0 g/l Yeast extract 3.0 g/l K2HPO4 1.5 g/l Na2S x 9 H2O 1.0 g/l NH4Cl 0.9 g/l KH2PO4 0.75 g/l MgCl2 x 6 H2O 0.4 g/l Nitrilotriacetic acid 0.1152 g/l NaCl 0.009 g/l FeSO4 x 7 H2O 0.003 g/l FeCl2 x 4 H2O 0.0018 g/l CoCl2 x 6 H2O 0.00153 g/l MnCl2 x 4 H2O 0.0009 g/l CaCl2 x 2 H2O 0.0009 g/l ZnCl2 0.0009 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.00027 g/l Na2SeO3 x 5 H2O 0.00027 g/l CuCl2 0.00018 g/l H3BO3 9e-05 g/l Na2MoO4 x 2 H2O 9e-05 g/l Pyridoxine hydrochloride 5e-05 g/l Calcium D-(+)-pantothenate 2.5e-05 g/l p-Aminobenzoic acid 2.5e-05 g/l (DL)-alpha-Lipoic acid 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l Thiamine HCl 2.5e-05 g/l Riboflavin 2.5e-05 g/l Biotin 1e-05 g/l Folic acid 1e-05 g/l Vitamin B12 5e-07 g/l Distilled water |
| 4691 | THERMOANAEROBACTER (KOKO) MEDIUM (DSMZ Medium 710) | yes | https://mediadive.dsmz.de/medium/710 | Name: THERMOANAEROBACTER (KoKo) MEDIUM (DSMZ Medium 710) Composition: D-Glucose 4.99002 g/l K2HPO4 1.59681 g/l NaHCO3 0.998004 g/l NaH2PO4 x 2 H2O 0.998004 g/l Yeast extract 0.998004 g/l Peptone 0.998004 g/l Tryptone 0.998004 g/l NH4Cl 0.499002 g/l Na2S x 9 H2O 0.299401 g/l L-Cysteine HCl x H2O 0.299401 g/l MgSO4 x 6 H2O 0.159681 g/l CaCl2 x 2 H2O 0.0598802 g/l Na2-EDTA x 2 H2O 0.00518962 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l CoCl2 x 6 H2O 0.000189621 g/l MnCl2 x 4 H2O 9.98004e-05 g/l Pyridoxine hydrochloride 9.98004e-05 g/l ZnCl2 6.98603e-05 g/l Thiamine HCl 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l Folic acid 1.99601e-05 g/l Biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water |
culture temp
- @ref: 4691
- growth: positive
- type: growth
- temperature: 70
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 4691
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 4691
- sample type: hydrothermal vent
- geographic location: Kamchatka peninsula, Karymsky volcano area
- country: Russia
- origin.country: RUS
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Hydrothermal vent |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 4691
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermoanaerobacter siderophilus SR4 | GCA_000262445 | chromosome | ncbi | 880478 |
| 66792 | Thermoanaerobacter siderophilus SR4 | 880478.3 | complete | patric | 880478 |
| 66792 | Thermoanaerobacter siderophilus SR4 | 2509276025 | draft | img | 880478 |
GC content
- @ref: 4691
- GC-content: 32
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | yes | 86.403 | no |
| flagellated | no | 79.724 | no |
| gram-positive | no | 69.859 | no |
| anaerobic | yes | 99.473 | no |
| halophile | no | 95.82 | no |
| spore-forming | yes | 92.591 | no |
| thermophile | yes | 100 | no |
| glucose-util | yes | 92.477 | no |
| aerobic | no | 98.726 | yes |
| glucose-ferment | yes | 56.798 | no |
External links
@ref: 4691
culture collection no.: DSM 12299
straininfo link
- @ref: 85854
- straininfo: 49268
literature
- topic: Phylogeny
- Pubmed-ID: 10555328
- title: Thermoanaerobacter siderophilus sp. nov., a novel dissimilatory Fe(III)-reducing, anaerobic, thermophilic bacterium.
- authors: Slobodkin AI, Tourova TP, Kuznetsov BB, Kostrikina NA, Chernyh NA, Bonch-Osmolovskaya EA
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-49-4-1471
- year: 1999
- mesh: Bacteria, Anaerobic/*classification/genetics/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Ferric Compounds/*metabolism, Geologic Sediments/*microbiology, Gram-Positive Asporogenous Rods, Irregular/*chemistry/genetics/physiology, Hot Temperature, Molecular Sequence Data, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Volcanic Eruptions
- topic2: Stress
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 4691 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12299) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-12299 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 85854 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49268.1 | StrainInfo: A central database for resolving microbial strain identifiers |