Workshop and Q&A October 6th

Strain identifier

BacDive ID: 16820

Type strain: Yes

Species: Moorella glycerini

Strain Designation: JW/AS-Y6

Strain history: <- J. Wiegel, University of Georgia, Department of Microbiology, Athens, USA; JW/AS-Y6 <- A. Slobodkin {1996}

NCBI tax ID(s): 55779 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4261

BacDive-ID: 16820

DSM-Number: 11254

keywords: anaerobe, Bacteria, 16S sequence, genome sequence, thermophilic

description: Moorella glycerini JW/AS-Y6 is an anaerobe, thermophilic bacterium that was isolated from mixed sediment-water sample of hot spring.

NCBI tax id

  • NCBI tax id: 55779
  • Matching level: species

strain history: <- J. Wiegel, University of Georgia, Department of Microbiology, Athens, USA; JW/AS-Y6 <- A. Slobodkin {1996}

doi: 10.13145/bacdive16820.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Thermoanaerobacterales
  • family: Thermoanaerobacteraceae
  • genus: Moorella
  • species: Moorella glycerini
  • full scientific name: Moorella glycerini Slobodkin et al. 1997

@ref: 4261

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Moorella

species: Moorella glycerini

full scientific name: Moorella glycerini Slobodkin et al. 1997

strain designation: JW/AS-Y6

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 4261
  • name: MOORELLA GLYCERINI MEDIUM (DSMZ Medium 793)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/793
  • composition: Name: MOORELLA GLYCERINI MEDIUM (DSMZ Medium 793) Composition: NaHCO3 10.0 g/l Glycerol 2.61 g/l Yeast extract 0.5 g/l Na2S x 9 H2O 0.5 g/l NH4Cl 0.33 g/l KCl 0.33 g/l MgCl2 x 6 H2O 0.33 g/l KH2PO4 0.33 g/l CaCl2 x 2 H2O 0.33 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l NaOH 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l Pyridoxine hydrochloride 0.0001 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l (±)-alpha-Lipoic acid 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l Nicotinic acid 5e-05 g/l Riboflavin 5e-05 g/l Thiamine HCl 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l H3BO3 6e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-06 g/l Distilled water

culture temp

  • @ref: 4261
  • growth: positive
  • type: growth
  • temperature: 60
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 4261
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 4261
  • sample type: mixed sediment-water sample of hot spring
  • geographic location: Yellowstone Natl. Park, Calcite Spring area
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 4261
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4261
  • description: Moorella glycerini 16S small subunit ribosomal RNA gene, complete sequence
  • accession: U82327
  • length: 1513
  • database: ena
  • NCBI tax ID: 55779

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Moorella glycerini DSM 11254GCA_009735625chromosomencbi55779
66792Moorella glycerini strain DSM 1125455779.5completepatric55779

GC content

  • @ref: 4261
  • GC-content: 54.5

External links

@ref: 4261

culture collection no.: DSM 11254

straininfo link

@refpassport
20218http://www.straininfo.net/strains/162558
20218http://www.straininfo.net/strains/162559

literature

  • topic: Phylogeny
  • Pubmed-ID: 9336894
  • title: Isolation and characterization of the homoacetogenic thermophilic bacterium Moorella glycerini sp. nov.
  • authors: Slobodkin A, Reysenbach AL, Mayer F, Wiegel J
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-47-4-969
  • year: 1997
  • mesh: Base Composition, DNA, Bacterial/analysis, Gram-Positive Bacteria/chemistry/*classification/genetics/isolation & purification/physiology/ultrastructure, Hydrogen-Ion Concentration, Microbial Sensitivity Tests, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/analysis, Sodium Chloride, Temperature
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4261Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11254)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11254
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)