Strain identifier
BacDive ID: 16820
Type strain: 
Species: Moorella glycerini
Strain Designation: JW/AS-Y6
Strain history: <- J. Wiegel, University of Georgia, Department of Microbiology, Athens, USA; JW/AS-Y6 <- A. Slobodkin {1996}
NCBI tax ID(s): 55779 (species)
version 8.1 (current version)
General
@ref: 4261
BacDive-ID: 16820
DSM-Number: 11254
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, motile
description: Moorella glycerini JW/AS-Y6 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from mixed sediment-water sample of hot spring.
NCBI tax id
- NCBI tax id: 55779
- Matching level: species
strain history
- @ref: 4261
- history: <- J. Wiegel, University of Georgia, Department of Microbiology, Athens, USA; JW/AS-Y6 <- A. Slobodkin {1996}
doi: 10.13145/bacdive16820.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Moorella
- species: Moorella glycerini
- full scientific name: Moorella glycerini Slobodkin et al. 1997
@ref: 4261
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Moorella
species: Moorella glycerini
full scientific name: Moorella glycerini Slobodkin et al. 1997
strain designation: JW/AS-Y6
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 97.008 | |
| 69480 | 99.72 | positive |
Culture and growth conditions
culture medium
- @ref: 4261
- name: MOORELLA GLYCERINI MEDIUM (DSMZ Medium 793)
- growth: yes
- link: https://mediadive.dsmz.de/medium/793
- composition: Name: MOORELLA GLYCERINI MEDIUM (DSMZ Medium 793) Composition: NaHCO3 9.94036 g/l Glycerol 2.59443 g/l Na2S x 9 H2O 0.497018 g/l Yeast extract 0.497018 g/l NH4Cl 0.328032 g/l KCl 0.328032 g/l MgCl2 x 6 H2O 0.328032 g/l CaCl2 x 2 H2O 0.328032 g/l KH2PO4 0.328032 g/l HCl 0.00248509 g/l FeCl2 x 4 H2O 0.00149105 g/l Sodium resazurin 0.000497018 g/l NaOH 0.000497018 g/l CoCl2 x 6 H2O 0.000188867 g/l Pyridoxine hydrochloride 9.94036e-05 g/l MnCl2 x 4 H2O 9.94036e-05 g/l ZnCl2 6.95825e-05 g/l p-Aminobenzoic acid 4.97018e-05 g/l Calcium D-(+)-pantothenate 4.97018e-05 g/l Nicotinic acid 4.97018e-05 g/l (DL)-alpha-Lipoic acid 4.97018e-05 g/l Riboflavin 4.97018e-05 g/l Thiamine HCl 4.97018e-05 g/l Na2MoO4 x 2 H2O 3.57853e-05 g/l NiCl2 x 6 H2O 2.38569e-05 g/l Biotin 1.98807e-05 g/l Folic acid 1.98807e-05 g/l H3BO3 5.96421e-06 g/l Na2WO4 x 2 H2O 3.97614e-06 g/l Na2SeO3 x 5 H2O 2.98211e-06 g/l CuCl2 x 2 H2O 1.98807e-06 g/l Vitamin B12 9.94036e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 4261 | positive | growth | 60 | thermophilic | |
| 69480 | thermophilic | 100 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 4261 | anaerobe | |
| 69480 | anaerobe | 99.705 |
spore formation
- @ref: 69480
- spore formation: yes
- confidence: 100
Isolation, sampling and environmental information
isolation
- @ref: 4261
- sample type: mixed sediment-water sample of hot spring
- geographic location: Yellowstone Natl. Park, Calcite Spring area
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_81169.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15934;96_746;97_869;98_56456;99_81169&stattab=map
- Last taxonomy: Moorella
- 16S sequence: U82327
- Sequence Identity:
- Total samples: 24
- soil counts: 11
- aquatic counts: 11
- animal counts: 2
Safety information
risk assessment
- @ref: 4261
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 4261
- description: Moorella glycerini 16S small subunit ribosomal RNA gene, complete sequence
- accession: U82327
- length: 1513
- database: ena
- NCBI tax ID: 55779
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Moorella glycerini DSM 11254 | GCA_009735625 | chromosome | ncbi | 55779 |
| 66792 | Moorella glycerini strain DSM 11254 | 55779.5 | complete | patric | 55779 |
GC content
- @ref: 4261
- GC-content: 54.5
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 62 | no |
| gram-positive | no | 80.011 | no |
| anaerobic | yes | 99.009 | no |
| halophile | no | 95.969 | no |
| spore-forming | yes | 90.5 | no |
| glucose-util | yes | 86.658 | no |
| aerobic | no | 96.738 | yes |
| thermophile | yes | 100 | yes |
| motile | yes | 87.379 | no |
| flagellated | no | 90.99 | no |
| glucose-ferment | no | 79.525 | no |
External links
@ref: 4261
culture collection no.: DSM 11254
straininfo link
- @ref: 85831
- straininfo: 49058
literature
- topic: Phylogeny
- Pubmed-ID: 9336894
- title: Isolation and characterization of the homoacetogenic thermophilic bacterium Moorella glycerini sp. nov.
- authors: Slobodkin A, Reysenbach AL, Mayer F, Wiegel J
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-47-4-969
- year: 1997
- mesh: Base Composition, DNA, Bacterial/analysis, Gram-Positive Bacteria/chemistry/*classification/genetics/isolation & purification/physiology/ultrastructure, Hydrogen-Ion Concentration, Microbial Sensitivity Tests, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/analysis, Sodium Chloride, Temperature
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 4261 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11254) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11254 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 85831 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49058.1 | StrainInfo: A central database for resolving microbial strain identifiers |