Strain identifier
BacDive ID: 16777
Type strain: 
Species: Thermoactinomyces intermedius
Strain Designation: T-323
Strain history: KCC A-0312 <-- V. P. Kurup T-323.
NCBI tax ID(s): 2024 (species)
version 8.1 (current version)
General
@ref: 11328
BacDive-ID: 16777
DSM-Number: 43846
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, thermophilic, Gram-positive
description: Thermoactinomyces intermedius T-323 is a spore-forming, thermophilic, Gram-positive bacterium that was isolated from air conditioner filter.
NCBI tax id
- NCBI tax id: 2024
- Matching level: species
strain history
| @ref | history |
|---|---|
| 11328 | <- KCC <- V.P. Kurup, T-323 |
| 67770 | KCC A-0312 <-- V. P. Kurup T-323. |
doi: 10.13145/bacdive16777.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Thermoactinomycetaceae
- genus: Thermoactinomyces
- species: Thermoactinomyces intermedius
- full scientific name: Thermoactinomyces intermedius Kurup et al. 1981
@ref: 11328
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Thermoactinomycetaceae
genus: Thermoactinomyces
species: Thermoactinomyces intermedius
full scientific name: Thermoactinomyces intermedius Kurup et al. 1981
strain designation: T-323
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 100
multimedia
- @ref: 11328
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43846.jpg
- caption: Medium 65 50°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 11328 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
| 11328 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 11328 | positive | growth | 50 | thermophilic | |
| 67770 | positive | growth | 50 | thermophilic | |
| 69480 | thermophilic | 95.692 |
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
observation
- @ref: 67770
- observation: quinones: MK-7
Isolation, sampling and environmental information
isolation
- @ref: 11328
- sample type: air conditioner filter
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Built environment | #Air conditioner |
| #Environmental | #Air |
taxonmaps
- @ref: 69479
- File name: preview.99_3092.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_358;96_1615;97_1932;98_2375;99_3092&stattab=map
- Last taxonomy: Thermoactinomyces
- 16S sequence: AF138734
- Sequence Identity:
- Total samples: 38731
- soil counts: 18726
- aquatic counts: 4962
- animal counts: 9694
- plant counts: 5349
Safety information
risk assessment
- @ref: 11328
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Thermoactinomyces intermedius partial 16S rRNA gene | AJ251775 | 1457 | ena | 2024 |
| 20218 | Actinomadura fastidiosa strain ATCC33516 16S ribosomal RNA gene, partial sequence | AF051390 | 189 | ena | 46173 |
| 20218 | Thermoactinomyces intermedius KCTC 9646 16S ribosomal RNA gene, complete sequence | AF138734 | 1504 | ena | 2024 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermoactinomyces intermedius CICC 10671=DSM 43846 | GCA_016055025 | contig | ncbi | 2024 |
| 66792 | Thermoactinomyces intermedius strain CICC 10671=DSM 43846 | 2024.5 | wgs | patric | 2024 |
| 66792 | Thermoactinomyces intermedius NBRC 14230 | 2784132173 | draft | img | 2024 |
GC content
- @ref: 67770
- GC-content: 48
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | no | 86.524 | no |
| gram-positive | yes | 91.951 | no |
| anaerobic | no | 97.622 | no |
| aerobic | yes | 91.747 | no |
| halophile | no | 78.572 | no |
| spore-forming | yes | 95.507 | no |
| glucose-util | yes | 92.224 | no |
| flagellated | no | 92.956 | no |
| thermophile | yes | 99.056 | no |
| glucose-ferment | no | 90.088 | no |
External links
@ref: 11328
culture collection no.: DSM 43846, ATCC 33205, DSM 44011, IFO 14230, JCM 3312, KCC A-0312, NBRC 14230, KCTC 3665, KCTC 9646, NRRL B-16979, VKM Ac-1427
straininfo link
- @ref: 85793
- straininfo: 40390
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Enzymology | 2061279 | Distribution, purification, and characterization of thermostable phenylalanine dehydrogenase from thermophilic actinomycetes. | Ohshima T, Takada H, Yoshimura T, Esaki N, Soda K | J Bacteriol | 10.1128/jb.173.13.3943-3948.1991 | 1991 | Actinomycetales/*enzymology, Amino Acid Oxidoreductases/chemistry/isolation & purification/*metabolism, Energy Transfer, Enzyme Stability, *Hot Temperature, Hydrogen/metabolism, Hydrogen-Ion Concentration, Kinetics, Molecular Weight, Phenylalanine/metabolism, Substrate Specificity | Metabolism |
| Enzymology | 8020469 | The purification, characterization, cloning and sequencing of the gene for a halostable and thermostable leucine dehydrogenase from Thermoactinomyces intermedius. | Ohshima T, Nishida N, Bakthavatsalam S, Kataoka K, Takada H, Yoshimura T, Esaki N, Soda K | Eur J Biochem | 10.1111/j.1432-1033.1994.tb18869.x | 1994 | Actinomyces/*enzymology/*genetics, Amino Acid Oxidoreductases/*genetics/isolation & purification/*metabolism, Amino Acid Sequence, Base Sequence, Chromatography, Affinity, Chromatography, Gel, Cloning, Molecular/methods, Electrophoresis, Polyacrylamide Gel, Enzyme Stability, Escherichia coli, *Genes, Bacterial, Hot Temperature, Hydrogen-Ion Concentration, Kinetics, Leucine Dehydrogenase, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Molecular Weight, NAD/metabolism, Recombinant Proteins/isolation & purification/metabolism, Stereoisomerism, Substrate Specificity, Thermodynamics, Time Factors | Metabolism |
| Enzymology | 10713207 | Synthesis of allysine ethylene acetal using phenylalanine dehydrogenase from Thermoactinomyces intermedius. | Hanson RL, Howell JM, LaPorte TL, Donovan MJ, Cazzulino DL, Zannella V V, Montana MA, Nanduri VB, Schwarz SR, Eiring RF, Durand SC, Wasylyk JM, Parker WL, Liu MS, Okuniewicz FJ, Chen B, Harris JC, Natalie KJ, Ramig K, Swaminathan S, Rosso VW, Pack SK, Lotz BT, Bernot PJ, Rusowicz A, Lust DA, Tse KS, Venit JJ, Szarka LJ, Patel RN | Enzyme Microb Technol | 10.1016/s0141-0229(99)00175-1 | 2000 | ||
| Phylogeny | 24048866 | Thermoactinomyces daqus sp. nov., a thermophilic bacterium isolated from high-temperature Daqu. | Yao S, Liu Y, Zhang M, Zhang X, Li H, Zhao T, Xin C, Xu L, Zhang B, Cheng C | Int J Syst Evol Microbiol | 10.1099/ijs.0.055509-0 | 2013 | Alcoholic Beverages/*microbiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Fermentation, Hot Temperature, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spores, Bacterial, Thermoactinomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry | Stress |
| Phylogeny | 25991661 | Thermoactinomyces guangxiensis sp. nov., a thermophilic actinomycete isolated from mushroom compost. | Wu H, Liu B, Pan S | Int J Syst Evol Microbiol | 10.1099/ijs.0.000342 | 2015 | *Agaricales, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, *Soil Microbiology, Spores, Bacterial, Thermoactinomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives | Genetics |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 11328 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43846) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-43846 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 85793 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID40390.1 | StrainInfo: A central database for resolving microbial strain identifiers |