Strain identifier

BacDive ID: 166519

Type strain: Yes

Species: Legionella qingyii

Strain Designation: km488

NCBI tax ID(s): 2184757 (species)

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General

@ref: 67958

BacDive-ID: 166519

keywords: genome sequence, Bacteria, Gram-negative, motile, rod-shaped, colony-forming

description: Legionella qingyii km488 is a Gram-negative, motile, rod-shaped bacterium that forms circular colonies and was isolated from river freshwater.

NCBI tax id

  • NCBI tax id: 2184757
  • Matching level: species

doi: 10.13145/bacdive166519.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella qingyii
  • full scientific name: Legionella qingyii Wu et al. 2019

@ref: 67958

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella qingyii

strain designation: km488

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
67958negative1.6-3.2 µm0.5-0.7 µmrod-shapedyes
69480negative99.998

colony morphology

  • @ref: 67958
  • colony size: 1 mm
  • colony shape: circular
  • incubation period: 7 days
  • medium used: BCYEalpha agar

pigmentation

  • @ref: 67958
  • production: yes
  • color: brwon
  • name: diffusible pigment

Culture and growth conditions

culture temp

@refgrowthtypetemperaturerange
67958positivegrowth25-42
67958positiveoptimum35mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
67958no
69480no99.989
69481no100

observation

@refobservation
67958Blue-white autofluorescence was observed under the Woods lamp
67958require L-cysteine for growth

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
679585291gelatin+assimilation
6795817234glucose-fermentation
6795817632nitrate-reduction
67958606565hippurate-fermentation

enzymes

@refvalueactivityec
67958catalase+1.11.1.6
67958beta-lactamase+3.5.2.6
67958cytochrome oxidase-1.9.3.1
67958urease-3.5.1.5
67958alkaline phosphatase+3.1.3.1
67958esterase+
67958leucine arylamidase+3.4.11.1
67958valine arylamidase+
67958acid phosphatase+3.1.3.2
67958naphthol-AS-BI-phosphohydrolase+
67958esterase Lipase (C 8)+/-
67958lipase+/-
67958cystine arylamidase+/-3.4.11.3
67958trypsin-3.4.21.4
67958alpha-chymotrypsin-3.4.21.1
67958alpha-galactosidase-3.2.1.22
67958beta-galactosidase-3.2.1.23
67958beta-glucuronidase-3.2.1.31
67958alpha-glucosidase-3.2.1.20
67958N-acetyl-beta-glucosaminidase-3.2.1.52
67958beta-glucosidase-3.2.1.21
67958alpha-mannosidase-3.2.1.24
67958alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    67958C14:02
    67958C15:03.3
    67958C16:08.3
    67958C17:04.8
    67958C18:01.9
    67958C14:0 iso2.9
    67958C15:0 anteiso25.9
    67958C16:0 iso14.5
    67958C17:0 anteiso13.8
    67958C15:1 w6c1.8
    67958C17:0 cyclo4.8
  • type of FA analysis: whole cell analysis
  • incubation medium: BCYEalpha
  • agar/liquid: agar
  • incubation temperature: 35
  • incubation time: 3
  • software version: Sherlock 6.2
  • library/peak naming table: TSBA6
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 67958
  • sample type: river freshwater
  • sampling date: 2013-08
  • geographic location: Majiagou River of Harbin city, Heilongjiang Province
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 45.55
  • longitude: 126.65

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella sp. HEB182184757.3wgspatric2184757
67958Legionella qingyii km488GCA_003184185scaffoldncbi2184757

GC content

  • @ref: 67958
  • GC-content: 37.8
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes91.143yes
gram-positiveno98.617yes
anaerobicno97.83no
aerobicno90.984no
halophileno90.207no
spore-formingno92.293yes
glucose-utilyes62.789no
flagellatedno62.145no
thermophileno98.224yes
glucose-fermentno88.451yes

External links

@ref: 67958

culture collection no.: KCTC 15636, CCTCC AB 2018025, NRBC 113223

literature

  • topic: Phylogeny
  • Pubmed-ID: 31063123
  • title: Legionella qingyii sp. nov., isolated from water samples in China.
  • authors: Wu HY, Yan H, Zheng ML, Sun MM, Wang Q, Hu CM, Zhan XY, Yuan MG, Qu PH, Hu CH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003421
  • year: 2019
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fresh Water/*microbiology, Genes, Bacterial, Legionella/*classification/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67958Hai-Yan Wu, Hui Ya, Min-Ling Zheng, Ming-Ming Sun, Qun Wang, Chang-Ming Hu, Xiao-Yong Zhan, Mu-Ge Yuan, Ping-Hua Qu, Chao-Hui HuLegionella qingyii sp. nov., isolated from water samples in China10.1099/ijsem.0.003421IJSEM 69: 2017-2022 2019
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1