Strain identifier
BacDive ID: 16651
Type strain: 
Species: Saccharolobus solfataricus
Strain Designation: P2
Strain history: DSM 1617 <-- W. Zillig P2.
NCBI tax ID(s): 2287 (species)
version 8.1 (current version)
General
@ref: 832
BacDive-ID: 16651
DSM-Number: 1617
keywords: genome sequence, 16S sequence, Archaea, thermophilic
description: Saccharolobus solfataricus P2 is a thermophilic archaeon that was isolated from volcanic hot spring.
NCBI tax id
- NCBI tax id: 2287
- Matching level: species
strain history
| @ref | history |
|---|---|
| 832 | <- W. Zillig, P2 |
| 67770 | DSM 1617 <-- W. Zillig P2. |
doi: 10.13145/bacdive16651.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Sulfolobales
- family: Sulfolobaceae
- genus: Saccharolobus
- species: Saccharolobus solfataricus
- full scientific name: Saccharolobus solfataricus (Zillig et al. 1980) Sakai and Kurosawa 2018
synonyms
- @ref: 20215
- synonym: Sulfolobus solfataricus
@ref: 832
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Sulfolobales
family: Sulfolobaceae
genus: Saccharolobus
species: Saccharolobus solfataricus
full scientific name: Saccharolobus solfataricus (Zillig et al. 1980) Sakai and Kurosawa 2018
strain designation: P2
type strain: no
Culture and growth conditions
culture medium
- @ref: 832
- name: SACCHAROLOBUS SOLFATARICUS MEDIUM (DSMZ Medium 182)
- growth: yes
- link: https://mediadive.dsmz.de/medium/182
- composition: Name: SACCHAROLOBUS SOLFATARICUS MEDIUM (DSMZ Medium 182) Composition: KH2PO4 3.1 g/l (NH4)2SO4 2.5 g/l Casamino acids 1.0 g/l Yeast extract 1.0 g/l CaCl2 x 2 H2O 0.25 g/l MgSO4 x 7 H2O 0.2 g/l Na2B4O7 x 10 H2O 0.0045 g/l MnCl2 x 4 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.00022 g/l CuCl2 x 2 H2O 5e-05 g/l VOSO4 x 2 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l CoSO4 x 7 H2O 1e-05 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 832 | positive | growth | 70 | thermophilic |
| 67770 | positive | growth | 70 | thermophilic |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 832 | volcanic hot spring | Campi flegrei, Pisciarelli near Agnano | Italy | ITA | Europe |
| 67770 | Volcanic hot spring at Pisciarelli Solfatara | Italy | ITA | Europe |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Environmental | #Terrestrial | #Volcanic |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 832
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20218
- description: Sulfolobus solfataricus 16S ribosomal RNA (16S rRNA) gene
- accession: L36991
- length: 527
- database: ena
- NCBI tax ID: 2287
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Saccharolobus solfataricus P2 | GCA_000007005 | complete | ncbi | 273057 |
| 66792 | Saccharolobus solfataricus P2 | 638154518 | complete | img | 273057 |
GC content
- @ref: 832
- GC-content: 35
- method: sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 81.969 | no |
| flagellated | no | 97.143 | no |
| gram-positive | no | 91.264 | no |
| anaerobic | yes | 68.211 | no |
| halophile | no | 83.302 | no |
| spore-forming | no | 95.363 | no |
| glucose-util | yes | 87.704 | no |
| aerobic | no | 66.766 | no |
| thermophile | yes | 100 | yes |
| glucose-ferment | no | 82.692 | no |
External links
@ref: 832
culture collection no.: DSM 1617, JCM 11322, ATCC 35092
straininfo link
- @ref: 85672
- straininfo: 40868
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Enzymology | 1463738 | Thermostable NAD(+)-dependent alcohol dehydrogenase from Sulfolobus solfataricus: gene and protein sequence determination and relationship to other alcohol dehydrogenases. | Ammendola S, Raia CA, Caruso C, Camardella L, D'Auria S, De Rosa M, Rossi M | Biochemistry | 10.1021/bi00164a031 | 1992 | Alcohol Dehydrogenase/*chemistry/*genetics/isolation & purification, Amino Acid Sequence, Base Sequence, Chromatography, High Pressure Liquid, Enzyme Stability, Genes, Bacterial, Hot Temperature, Molecular Sequence Data, NAD/metabolism, Sequence Homology, Amino Acid, Sulfolobus/*enzymology/genetics | Stress |
| Enzymology | 8550434 | Cloning and overexpression in Escherichia coli of the genes encoding NAD-dependent alcohol dehydrogenase from two Sulfolobus species. | Cannio R, Fiorentino G, Carpinelli P, Rossi M, Bartolucci S | J Bacteriol | 10.1128/jb.178.1.301-305.1996 | 1996 | Alcohol Dehydrogenase/biosynthesis/*genetics/isolation & purification/metabolism, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Escherichia coli/*genetics, Genes, Bacterial/*genetics, Hot Temperature, Kinetics, Molecular Sequence Data, Recombinant Fusion Proteins/biosynthesis/isolation & purification, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Sulfolobus/enzymology/*genetics | Phylogeny |
| Enzymology | 9311113 | Identification and sequence analysis of Sulfolobus solfataricus purE and purK genes. | Sorensen IS, Dandanell G | FEMS Microbiol Lett | 10.1111/j.1574-6968.1997.tb12640.x | 1997 | Amino Acid Sequence, Bacterial Proteins/genetics, Base Sequence, Carboxy-Lyases/*genetics, Cloning, Molecular, Escherichia coli/genetics, *Escherichia coli Proteins, *Genes, Bacterial, Genetic Complementation Test, Molecular Sequence Data, Mutation, Purines/*biosynthesis, Sulfolobus/*genetics | Genetics |
| Enzymology | 12072960 | A new type of dihydroorotate dehydrogenase, type 1S, from the thermoacidophilic archaeon Sulfolobus solfataricus. | Sorensen G, Dandanell G | Extremophiles | 10.1007/s00792-001-0249-0 | 2002 | Dihydroorotate Dehydrogenase, Electrophoresis, Polyacrylamide Gel, Kinetics, Oxidoreductases/chemistry/*metabolism, *Oxidoreductases Acting on CH-CH Group Donors, Substrate Specificity, Sulfolobus/*enzymology | Metabolism |
| Metabolism | 15150700 | Characterization of the trehalosyl dextrin-forming enzyme from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092. | Fang TY, Hung XG, Shih TY, Tseng WC | Extremophiles | 10.1007/s00792-004-0393-4 | 2004 | Amino Acid Sequence, Archaeal Proteins/*metabolism, Base Sequence, Chromatography, Gel, Conserved Sequence, DNA Primers, Dextrins/*biosynthesis/*metabolism, Enzymes/isolation & purification/*metabolism, Genetic Vectors, Hot Temperature, Molecular Sequence Data, Polymerase Chain Reaction, Restriction Mapping, Sequence Alignment, Sequence Homology, Amino Acid, Sulfolobus solfataricus/*enzymology/genetics, Thermodynamics, Trehalose/genetics/*metabolism, Ultrafiltration, alpha-Amylases/*metabolism | Enzymology |
| Enzymology | 15999201 | Highly enantioselective stereo-inverting sec-alkylsulfatase activity of hyperthermophilic Archaea. | Wallner SR, Nestl BM, Faber K | Org Biomol Chem | 10.1039/b504883d | 2005 | Archaea/*enzymology, Molecular Conformation, Molecular Structure, Stereoisomerism, Sulfatases/*chemistry/metabolism, Temperature | Metabolism |
| 16232474 | Inhibitory effect of mineral ion accumulation on high density growth of the hyperthermophilic archaeon Sulfolobus solfataricus. | Park CB, Lee SB | J Biosci Bioeng | 10.1016/s1389-1723(99)80038-3 | 1999 | |||
| 16232752 | Effects of exogenous compatible solutes on growth of the hyperthermophilic archaeon Sulfolobus solfataricus. | Park CB, Lee SB | J Biosci Bioeng | 10.1016/s1389-1723(00)88952-5 | 2000 | |||
| Enzymology | 16968069 | Expression, purification, and characterization of the maltooligosyltrehalose trehalohydrolase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092. | Fang TY, Tseng WC, Guo MS, Shih TY, Hung XG | J Agric Food Chem | 10.1021/jf061318z | 2006 | Amino Acid Sequence, Cloning, Molecular, Escherichia coli/genetics, *Gene Expression, Glucosidases/*genetics/isolation & purification/*metabolism, Hydrogen-Ion Concentration, Recombinant Proteins, Substrate Specificity, Sulfolobus solfataricus/*enzymology/genetics, Temperature, Trehalose/biosynthesis | Phylogeny |
| Metabolism | 17485831 | TreX from Sulfolobus solfataricus ATCC 35092 displays isoamylase and 4-alpha-glucanotransferase activities. | Park HS, Park JT, Kang HK, Cha H, Kim DS, Kim JW, Park KH | Biosci Biotechnol Biochem | 10.1271/bbb.70016 | 2007 | Genes, Bacterial, Glucans/biosynthesis/chemistry, Glycogen Debranching Enzyme System/*genetics/metabolism, Isoamylase/*metabolism, Operon, Sulfolobus solfataricus/enzymology/*genetics, Trehalose/*biosynthesis | Enzymology |
| Metabolism | 18563901 | Identification of the essential catalytic residues and selectivity-related residues of maltooligosyltrehalose trehalohydrolase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092. | Fang TY, Tseng WC, Shih TY, Wang MY | J Agric Food Chem | 10.1021/jf073320b | 2008 | Binding Sites, Catalysis, Glucose/metabolism, Glucosidases/*chemistry/genetics/*metabolism, Models, Molecular, Mutagenesis, Site-Directed, Starch/metabolism, Structure-Activity Relationship, Substrate Specificity, Sulfolobus/*enzymology, Trehalose/metabolism | Enzymology |
| Enzymology | 24564903 | Identification of substrate-binding and selectivity-related residues of maltooligosyltrehalose synthase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092. | Tseng WC, Lin CR, Hung XG, Wei TY, Chen YC, Fang TY | Enzyme Microb Technol | 10.1016/j.enzmictec.2014.01.003 | 2014 | Amino Acid Sequence, Amino Acid Substitution, Archaeal Proteins/chemistry/genetics/isolation & purification/*metabolism, Catalysis, Catalytic Domain, Glucosyltransferases/chemistry/genetics/isolation & purification/*metabolism, Glycosylation, Hydrogen Bonding, Hydrolysis, Kinetics, Models, Molecular, Molecular Docking Simulation, Molecular Sequence Data, Mutagenesis, Site-Directed, Protein Binding, Protein Conformation, Sequence Alignment, Sequence Homology, Amino Acid, Species Specificity, Substrate Specificity, Sulfolobus solfataricus/*enzymology/genetics | Phylogeny |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 832 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1617) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-1617 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 85672 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID40868.1 | StrainInfo: A central database for resolving microbial strain identifiers |