Strain identifier

BacDive ID: 166283

Type strain: Yes

Species: Leucothrix sargassi

Strain Designation: C3212

NCBI tax ID(s): 2500160 (species)

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General

@ref: 68042

BacDive-ID: 166283

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Leucothrix sargassi C3212 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from marine alga collected on the sea shore.

NCBI tax id

  • NCBI tax id: 2500160
  • Matching level: species

doi: 10.13145/bacdive166283.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Beggiatoales
  • family: Leucotrichaceae
  • genus: Leucothrix
  • species: Leucothrix sargassi
  • full scientific name: Leucothrix sargassi Liu et al. 2019

@ref: 68042

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Thiotrichales

family: Thiotrichaceae

genus: Leucothrix

species: Leucothrix sargassi

strain designation: C3212

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
68042negative0.8-2.0 µm0.4-0.6 µmrod-shapedno
69480negative97.625

colony morphology

  • @ref: 68042
  • colony size: 0.3-0.6 mm
  • colony color: white
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Marine agar (MA)

pigmentation

  • @ref: 68042
  • production: no
  • name: Flexirubin type pigments

Culture and growth conditions

culture temp

@refgrowthtypetemperature
68042positivegrowth8-37
68042positiveoptimum28

culture pH

@refabilitytypepHPH range
68042positivegrowth6.0-9.0alkaliphile
68042positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 68042
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
68042NaClpositivegrowth1.0-7.0 %(w/v)
68042NaClpositiveoptimum4 %(w/v)

observation

@refobservation
68042The major respiratory quinone is Q-8
68042The polar lipids consist of diphosphatidylglycerol (cardiolipin), phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified polar lipid
68042Requires seawater or artificial seawater for growth, and NaCl alone does not support growth

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6804217632nitrate+reduction
680424853esculin+hydrolysis
6804253424tween 20+hydrolysis
6804253423tween 40+hydrolysis
6804253425tween 60+hydrolysis
6804253426tween 80+hydrolysis
6804216991dna-hydrolysis
6804216199urea-hydrolysis
680422509agar-hydrolysis
6804217029chitin-hydrolysis
680425291gelatin-hydrolysis
68042casein-hydrolysis
6804228017starch-hydrolysis
6804216024D-mannose-assimilation
6804217634D-glucose-assimilation
6804230849L-arabinose-assimilation
6804216899D-mannitol-assimilation
6804259640N-acetylglucosamine-assimilation
6804217306maltose-assimilation
6804232032potassium gluconate-assimilation
6804227689decanoate-assimilation
6804217128adipate-assimilation
6804225115malate-assimilation
6804253258sodium citrate-assimilation
6804218401phenylacetate-assimilation
6804217754glycerol+carbon source
6804285146carboxymethylcellulose+carbon source
6804217992sucrose+carbon source
6804253258sodium citrate+carbon source
6804232954sodium acetate+carbon source
6804253311sodium alginate+carbon source
6804253423tween 40+carbon source
6804253425tween 60+carbon source
6804253426tween 80+carbon source
6804217268myo-inositol+carbon source
6804216958beta-alanine+carbon source
6804217203L-proline+carbon source
6804217306maltose-carbon source
6804265327D-xylose-carbon source
6804212936D-galactose-carbon source
6804217716lactose-carbon source
6804217057cellobiose-carbon source
68042113455sodium benzoate-carbon source
6804215971L-histidine-carbon source
6804217295L-phenylalanine-carbon source
6804218019L-lysine-carbon source
6804216449dl-alanine-carbon source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.group IDis resistantresistance conc.
6804217334penicillinyesyes10 µg (disc)
6804228971ampicillinyesyes10 µg (disc)20
680429321sulbactamyesyes10 µg (disc)20
680423393carbenicillinyesyes100 µg (disc)
680428232piperacillinyesyes100 µg (disc)
680423534cephalexinyesyes30 µg (disc)
680423515cefuroximeyesyes30 µg (disc)
6804248923erythromycinyesyes15 µg (disc)
6804250694minocyclineyesyes30 µg (disc)
6804250845doxycyclineyesyes30 µg (disc)
6804227902tetracyclineyesyes30 µg (disc)
680423493cefoperazoneyesyes75 µg (disc)
6804229007ceftriaxoneyesyes30 µg (disc)
6804231845midecamycinyesyes30 µg (disc)
680427731ofloxacinyesyes5 µg (disc)
68042100241ciprofloxacinyesyes5 µg (disc)
6804228001vancomycinyesyes30 µg (disc)
6804217698chloramphenicolyesyes30 µg (disc)
680423745clindamycinyesyes2 µg (disc)
6804249566cloxacillinyesyes1 µg (disc)
680423508ceftazidimeyesyes30 µg (disc)
680427507neomycinyesyes30 µg (disc)
680426104kanamycinyesyes30 µg (disc)
6804217833gentamicinyesyes10 µg (disc)
680422637amikacinyesyes30 µg (disc)
68042100246norfloxacinyesyes10 µg (disc)
680428309polymyxin byesyes300 µg (disc)
68042474053cefazolinyesyes20 µg (disc)
680423547cephradineyesyes30 µg (disc)
680425195furazolidoneyesyes30 µg (disc)
6804245924trimethoprimyes26yes25 µg (disc)
680429332sulfamethoxazoleyes26yes25 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
6804216136hydrogen sulfideno
6804235581indoleno

enzymes

@refvalueactivityec
68042cytochrome oxidase+1.9.3.1
68042catalase+1.11.1.6
68042alkaline phosphatase+3.1.3.1
68042esterase (C 4)+
68042esterase Lipase (C 8)+
68042leucine arylamidase+3.4.11.1
68042acid phosphatase+3.1.3.2
68042naphthol-AS-BI-phosphohydrolase+
68042lipase (C 14)-
68042valine arylamidase-
68042cystine arylamidase-3.4.11.3
68042trypsin-3.4.21.4
68042alpha-chymotrypsin-3.4.21.1
68042alpha-galactosidase-3.2.1.22
68042beta-galactosidase-3.2.1.23
68042beta-glucuronidase-3.2.1.31
68042alpha-glucosidase-3.2.1.20
68042beta-glucosidase-3.2.1.21
68042N-acetyl-beta-glucosaminidase-3.2.1.52
68042alpha-mannosidase-3.2.1.24
68042alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    68042C16:03
    68042C17:02.6
    68042C18:01.1
    68042C17:1 w8c1.8
    68042C10:0 3OH3.7
    68042C16:1 w7c / C16:1 w6c34.2
    68042C18:1 w7c / C18:1 w6c48
    68042C17:1 iso I/C17:1 anteiso B1.1
  • type of FA analysis: whole cell analysis
  • incubation medium: MA
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 3.5
  • software version: Sherlock 6.1
  • library/peak naming table: RTSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 68042
  • sample type: marine alga (Sargassum natans) collected on the sea shore
  • sampling date: 2017-12
  • geographic location: Yantai
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 37.9833
  • longitude: 121.367

Sequence information

16S sequences

  • @ref: 68042
  • description: Leucothrix sargassi strain C3212 16S ribosomal RNA gene, partial sequence
  • accession: MK100847
  • length: 1502
  • database: nuccore
  • NCBI tax ID: 2500160

Genome sequences

  • @ref: 68042
  • description: Leucothrix sargassi C3212
  • accession: GCA_004010795
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 2500160

GC content

  • @ref: 68042
  • GC-content: 44.3
  • method: genome sequence analysis

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingno97.625yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no96.632no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes76.778no
69480spore-formingspore-formingAbility to form endo- or exosporesno83.406no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.988yes
69480flagellatedmotile2+Ability to perform flagellated movementno58.989yes

External links

@ref: 68042

culture collection no.: MCCC 1K03600, KCTC 72121

literature

  • topic: Phylogeny
  • Pubmed-ID: 31486761
  • title: Leucothrix sargassi sp. nov., isolated from a marine alga [Sargassum natans (L.) Gaillon].
  • authors: Liu T, Zhang Y, Zhang X, Zhou L, Meng C, Zhou C, Jing C, Zhu W, Wang Y
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003694
  • year: 2019
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sargassum/*microbiology, Sequence Analysis, DNA, Thiotrichaceae/*classification/isolation & purification, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
68042Tingwei Liu, Yao Zhang, Xuan Zhang, Lixin Zhou, Chunyu Meng, Chenyan Zhou, Changqin Jing, Wuling Zhu, Yan WangLeucothrix sargassi sp. nov., isolated from a marine alga [Sargassum natans (L.) Gaillon]10.1099/ijsem.0.003694IJSEM 69: 3857-3862 2019
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes