Strain identifier

BacDive ID: 166252

Type strain: Yes

Species: Thermomonas aquatica

Strain Designation: SY21

NCBI tax ID(s): 2202149 (species)

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General

@ref: 68206

BacDive-ID: 166252

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped

description: Thermomonas aquatica SY21 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from sewage treatment plant.

NCBI tax id

  • NCBI tax id: 2202149
  • Matching level: species

doi: 10.13145/bacdive166252.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Lysobacterales
  • family: Lysobacteraceae
  • genus: Thermomonas
  • species: Thermomonas aquatica
  • full scientific name: Thermomonas aquatica Ju et al. 2019

@ref: 68206

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Lysobacterales

family: Lysobacteraceae

genus: Thermomonas

species: Thermomonas aquatica

strain designation: SY21

type strain: yes

Morphology

cell morphology

  • @ref: 68206
  • gram stain: negative
  • cell length: 2.2-3.6 µm
  • cell width: 0.6-0.7 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 68206
  • type of hemolysis: gamma
  • colony color: translucent creamy white
  • incubation period: 3 days
  • medium used: Reasoner's 2A agar (R2A)

Culture and growth conditions

culture temp

@refgrowthtypetemperaturerange
68206positivegrowth15-40
68206positiveoptimum28mesophilic

culture pH

@refabilitytypepHPH range
68206positivegrowth6-9alkaliphile
68206positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 68206
  • oxygen tolerance: aerobe

halophily

  • @ref: 68206
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6820629016arginine+assimilation
6820616199urea+assimilation
682064853esculin+assimilation
6820617234glucose+assimilation
6820637684mannose+assimilation
6820629864mannitol+assimilation
6820617306maltose+assimilation
6820622599arabinose+/-assimilation
68206506227N-acetyl-D-glucosamine+/-assimilation
6820665327D-xylose-builds acid from
6820616988D-ribose-builds acid from
68206potassium 5-dehydro-D-gluconate+builds acid from
6820630849L-arabinose-builds acid from
6820616443D-tagatose-builds acid from
6820617634D-glucose-builds acid from
6820617306maltose-builds acid from
68206potassium 2-dehydro-D-gluconate-builds acid from
6820627082trehalose-builds acid from
6820632528turanose-builds acid from
6820612936D-galactose-builds acid from
6820615824D-fructose-builds acid from
6820617992sucrose-builds acid from
6820617057cellobiose-builds acid from
6820628053melibiose-builds acid from
6820618305arbutin-builds acid from
6820616024D-mannose-builds acid from
6820617814salicin-builds acid from
6820617716lactose-builds acid from
6820616899D-mannitol-builds acid from
6820628087glycogen-builds acid from
6820628017starch-builds acid from
6820617108D-arabinose-builds acid from
682066731melezitose-builds acid from
6820618287L-fucose-builds acid from
6820662345L-rhamnose-builds acid from
6820628066gentiobiose-builds acid from
6820617924D-sorbitol-builds acid from
6820618333D-arabitol-builds acid from
6820662318D-lyxose-builds acid from
6820628847D-fucose-builds acid from
6820617754glycerol-builds acid from
6820659640N-acetylglucosamine-builds acid from
68206320061methyl alpha-D-glucopyranoside-builds acid from
6820617268myo-inositol-builds acid from
6820632032potassium gluconate-builds acid from
6820674863methyl beta-D-xylopyranoside-builds acid from
6820616634raffinose-builds acid from
6820627613amygdalin-builds acid from
6820643943methyl alpha-D-mannoside-builds acid from
6820617113erythritol-builds acid from
6820665328L-xylose-builds acid from
6820615963ribitol-builds acid from
6820617266L-sorbose-builds acid from
6820616813galactitol-builds acid from
6820615443inulin-builds acid from
6820617151xylitol-builds acid from
6820618403L-arabitol-builds acid from
682064853esculin+builds acid from
6820640585alpha-cyclodextrin+oxidation
6820628087glycogen+oxidation
68206506227N-acetyl-D-glucosamine+oxidation
6820616634raffinose+oxidation
682068295beta-hydroxybutyrate+oxidation
68206168102-oxoglutarate+oxidation
6820616977L-alanine+oxidation
6820673786L-alanylglycine+oxidation
6820629985L-glutamate+oxidation
6820673804glycyl L-aspartic acid+oxidation
6820673784glycyl-l-glutamate+oxidation
6820617203L-proline+oxidation
6820617596inosine+oxidation
6820616704uridine+oxidation
6820617057cellobiose+/-oxidation
6820615824D-fructose+/-oxidation
6820616024D-mannose+/-oxidation
6820675146monomethyl succinate+/-oxidation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
682063732clarithromycinyesyes15 µg (disc)
6820629007ceftriaxoneyesyes30 µg (disc)
68206471744imipenemyesyes10 µg (disc)
6820650694minocyclineyesyes30 µg (disc)
6820628368novobiocinyesyes30 µg (disc)
682066472lincomycinyesyes15 µg (disc)
682062676amoxicillinyesyes30 µg (disc)
6820617076streptomycinyesyes10 µg (disc)
6820628001vancomycinyesyes30 µg (disc)
682067507neomycinyesyes30 µg (disc)
68206100147nalidixic acidyesyes30 µg (disc)
6820617698chloramphenicolyesyes30 µg (disc)
682066104kanamycinyesyes30 µg (disc)
6820648923erythromycinyesyes15 µg (disc)
6820628971ampicillinyesyes10 µg (disc)
6820627902tetracyclineyesyes30 µg (disc)
6820617833gentamicinyesyes10 µg (disc)
6820618208penicillin gyesyes10 µg (disc)
682063393carbenicillinyesyes100 µg (disc)

enzymes

@refvalueactivityec
68206catalase+1.11.1.6
68206cytochrome oxidase+1.9.3.1
68206nitrate reductase-1.7.99.4
68206alkaline phosphatase+3.1.3.1
68206esterase (C 4)+
68206esterase Lipase (C 8)+
68206leucine arylamidase+3.4.11.1
68206valine arylamidase+
68206cystine arylamidase+3.4.11.3
68206trypsin+3.4.21.4
68206acid phosphatase+3.1.3.2
68206naphthol-AS-BI-phosphohydrolase+
68206alpha-glucosidase+3.2.1.20
68206beta-glucosidase+3.2.1.21
68206lipase (C 14)-
68206alpha-chymotrypsin-3.4.21.1
68206alpha-galactosidase-3.2.1.22
68206beta-galactosidase-3.2.1.23
68206beta-glucuronidase-3.2.1.31
68206N-acetyl-beta-glucosaminidase-3.2.1.52
68206alpha-mannosidase-3.2.1.24
68206alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    68206C10:02.5
    68206C11:0 iso7.8
    68206C14:04.3
    68206C15:0 iso27.9
    68206C15:0 anteiso1.9
    68206C16:013.4
    68206C16:0 iso2.3
    68206C17:0 iso1.7
    68206C15:1 iso- w6c /C15:1 iso w5c1.3
    68206C10:0 3OH1.8
    68206C11:0 iso 3-OH10.3
    68206C16:1 w7c/C16:1 iso w6c11.7
    68206C17:1 iso w9c / C16:0 10-methyl12.3
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • incubation temperature: 25
  • system: MIS MIDI
  • instrument: 6890 N, Agilent
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 68206
  • sample type: sewage treatment plant
  • sampling date: 2017-08-08
  • geographic location: Nakdong River
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia
  • latitude: 36.0864
  • longitude: 128.955
  • enrichment culture: R2A agar
  • enrichment culture duration: 10 days
  • enrichment culture temperature: 25

Sequence information

16S sequences

  • @ref: 68206
  • description: Thermomonas sp. strain SY-21 16S ribosomal RNA gene, partial sequence
  • accession: MH396008
  • length: 1467
  • database: ena
  • NCBI tax ID: 1971895

Genome sequences

  • @ref: 68206
  • description: Thermomonas aquatica SY21
  • accession: GCA_006337105
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 2202149

GC content

  • @ref: 68206
  • GC-content: 67.9
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno89.569no
flagellatedno97.431yes
gram-positiveno98.819no
anaerobicno99.12yes
aerobicyes82.933no
halophileno95.766no
spore-formingno96.572no
glucose-utilyes84.285no
thermophileno97.417no
glucose-fermentno89.381no

External links

@ref: 68206

culture collection no.: KCTC 62191, NBRC 113114

literature

  • topic: Phylogeny
  • Pubmed-ID: 31380735
  • title: Thermomonas aquatica sp. nov., isolated from an industrial wastewater treatment plant.
  • authors: Ju JH, Kim JS, Lee DH, Jeon JH, Heo SY, Seo JW, Kim CH, Park DS, Oh BR
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003630
  • year: 2019
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/chemistry, Waste Water/*microbiology, Xanthomonadaceae/*classification/isolation & purification
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
68206Jung-Hyun Ju, Ji-Sun Kim, Dae-Hee Lee, Jun Hyoung Jeon, Sun-Yeon Heo, Jeong-Woo Seo, Chul Ho Kim, Doo-Sang Park and Baek-Rock OhThermomonas aquatica sp. nov., isolated from an industrial wastewater treatment plant10.1099/ijsem.0.003630IJSEM 69: 3399-3404 2019