Strain identifier

BacDive ID: 166098

Type strain: Yes

Species: Mucilaginibacter hurinus

Strain Designation: ZR32

NCBI tax ID(s): 2201324 (species)

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General

@ref: 68255

BacDive-ID: 166098

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Mucilaginibacter hurinus ZR32 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from soil of a warehouse which stored briquettes.

NCBI tax id

  • NCBI tax id: 2201324
  • Matching level: species

doi: 10.13145/bacdive166098.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Sphingobacteriia
  • order: Sphingobacteriales
  • family: Sphingobacteriaceae
  • genus: Mucilaginibacter
  • species: Mucilaginibacter hurinus
  • full scientific name: Mucilaginibacter hurinus Choi et al. 2020

@ref: 68255

domain: Bacteria

phylum: Bacteroidetes

class: Sphingobacteriia

order: Sphingobacteriales

family: Sphingobacteriaceae

genus: Mucilaginibacter

species: Mucilaginibacter hurinus

strain designation: ZR32

type strain: yes

Morphology

cell morphology

  • @ref: 68255
  • gram stain: negative
  • cell length: 0.6-0.7 µm
  • cell width: 0.4-0.5 µm
  • cell shape: rod-shaped

colony morphology

  • @ref: 68255
  • colony color: pink
  • colony shape: circular
  • incubation period: 3 days
  • medium used: Reasoner's 2A agar (R2A)

pigmentation

  • @ref: 68255
  • production: no
  • name: Flexirubin type pigments

Culture and growth conditions

culture temp

@refgrowthtypetemperature
68255positivegrowth4-28
68255positiveoptimum28

culture pH

@refabilitytypepHPH range
68255positivegrowth6-10alkaliphile
68255positiveoptimum6-7

Physiology and metabolism

oxygen tolerance

  • @ref: 68255
  • oxygen tolerance: aerobe

spore formation

  • @ref: 68255
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
68255NaClpositivegrowth0-1 %(w/v)
68255NaClpositiveoptimum0 %(w/v)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68255casein+hydrolysis
68255gelatin-hydrolysis5291
68255cellulose-hydrolysis62968
68255starch-hydrolysis28017
68255dna-hydrolysis16991
68255tween 20-hydrolysis53424
68255tween 60-hydrolysis53425
68255tween 80-hydrolysis53426
68255nitrate-reduction17632
68255citrate+assimilation16947
68255glucose-fermentation17234
68255glucose+assimilation17234
68255mannose+assimilation37684
68255maltose+assimilation17306
68255mannose-assimilation37684
68255mannitol-assimilation29864
68255N-acetylglucosamine-assimilation59640
68255potassium gluconate-assimilation32032
68255hexanoate-assimilation17120
68255adipate-assimilation17128
68255malate-assimilation25115
68255sodium citrate-assimilation53258
68255phenylacetate-assimilation18401

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
682553547cephradineyesyes
682553493cefoperazoneyesyes75 µg (disc)
682552637amikacinyesyes30 µg (disc)
6825517833gentamicinyesyes10 µg (disc)
682557507neomycinyesyes30 µg (disc)
6825527902tetracyclineyesyes30 µg (disc)
6825550845doxycyclineyesyes30 µg (disc)
6825550694minocyclineyesyes30 µg (disc)
6825517334penicillinyesyes10 Unit (disc)
682557809oxacillinyesyes10 µg (disc)
6825528971ampicillinyesyes10 µg (disc)
682553393carbenicillinyesyes100 µg (disc)
682558232piperacillinyesyes100 µg (disc)
682553515cefuroximeyesyes30 µg (disc)
682553508ceftazidimeyesyes30 µg (disc)
6825529007ceftriaxoneyesyes30 µg (disc)
682556104kanamycinyesyes30 µg (disc)
6825548923erythromycinyesyes15 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
6825535581indoleno
6825516136hydrogen sulfideno
6825515688acetoinno

metabolite tests

  • @ref: 68255
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68255cytochrome oxidase+1.9.3.1
68255catalase-1.11.1.6
68255alkaline phosphatase+3.1.3.1
68255esterase (C 4)+
68255esterase Lipase (C 8)+
68255leucine arylamidase+3.4.11.1
68255valine arylamidase+
68255cystine arylamidase+3.4.11.3
68255acid phosphatase+3.1.3.2
68255naphthol-AS-BI-phosphohydrolase+
68255alpha-glucosidase+3.2.1.20
68255N-acetyl-beta-glucosaminidase+3.2.1.52
68255alpha-mannosidase+3.2.1.24
68255lipase (C 14)-
68255trypsin-3.4.21.4
68255alpha-chymotrypsin-3.4.21.1
68255alpha-galactosidase-3.2.1.22
68255beta-galactosidase-3.2.1.23
68255beta-glucuronidase-3.2.1.31
68255alpha-fucosidase-3.2.1.51
68255beta-glucosidase+3.2.1.21
68255urease-3.5.1.5
68255arginine dihydrolase-3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    68255C16:05.3
    68255C16:0 3OH2.9
    68255C16:1 w5c7.6
    68255C17:1 w8c1.1
    68255C15:0 iso24.8
    68255C15:0 iso 3OH1.8
    68255C17:0 iso3.6
    68255C17:0 iso 3OH13
    68255C17:1 iso w9c7.2
    68255C17:1 anteiso B/iso-C17:1 I1.2
    68255C16:1 w6c/C16:1 w7c30.4
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • incubation temperature: 28
  • software version: Sherlock 4.5
  • library/peak naming table: TSBA40 4.10
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 68255
  • sample type: soil of a warehouse which stored briquettes
  • geographic location: Ulsan
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia
  • latitude: 35.5667
  • longitude: 129.083
  • enrichment culture: R2A agar
  • enrichment culture duration: 1 week
  • enrichment culture temperature: 28

Sequence information

16S sequences

  • @ref: 68255
  • description: Mucilaginibacter hurinus 16S ribosomal RNA gene, partial sequence
  • accession: MF919638
  • length: 1491
  • database: nuccore
  • NCBI tax ID: 2201324

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mucilaginibacter hurinus ZR322839151031draftimg2201324
68255Mucilaginibacter hurinus ZR32GCA_003324525contigncbi2201324

GC content

  • @ref: 68255
  • GC-content: 43
  • method: genome sequence analysis

External links

@ref: 68255

culture collection no.: KCTC 62193, CCTCC AB 2017285

literature

  • topic: Phylogeny
  • Pubmed-ID: 31515591
  • title: Mucilaginibacter hurinus sp. nov., isolated from briquette warehouse soil.
  • authors: Choi L, Zhao X, Song Y, Wu M, Wang G, Li M
  • journal: Arch Microbiol
  • DOI: 10.1007/s00203-019-01720-1
  • year: 2019
  • mesh: Bacterial Typing Techniques, Bacteroidetes/chemistry/*classification/genetics, Base Composition, DNA, Bacterial/genetics, Lipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Species Specificity
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68255Lina Choi, Xinran Zhao, Yali Song, Minghan Wu, Gejiao Wang and Mingshun LiMucilaginibacter hurinus sp. nov., isolated from briquette warehouse soil10.1007/s00203-019-01720-1Arch Microbiol 202: 127-134 2020