Strain identifier

BacDive ID: 16589

Type strain: Yes

Species: Streptosporangium roseum

Strain Designation: N I 9100, UNCC 27 B

Strain history: KCC A-0005 <-- NI 9100 <-- J. N. Couch 27-b (UNCC 27).

NCBI tax ID(s): 479432 (strain), 2001 (species)

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General

@ref: 10692

BacDive-ID: 16589

DSM-Number: 43021

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic

description: Streptosporangium roseum N I 9100 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from vegetable garden soil.

NCBI tax id

NCBI tax idMatching level
2001species
479432strain

strain history

@refhistory
10692<- KCC <- K. Tubaki, N I 9100 <- J.N. Couch, UNCC 27 B
67770KCC A-0005 <-- NI 9100 <-- J. N. Couch 27-b (UNCC 27).

doi: 10.13145/bacdive16589.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Streptosporangiaceae
  • genus: Streptosporangium
  • species: Streptosporangium roseum
  • full scientific name: Streptosporangium roseum Couch 1955 (Approved Lists 1980)

@ref: 10692

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptosporangiaceae

genus: Streptosporangium

species: Streptosporangium roseum

full scientific name: Streptosporangium roseum Couch 1955 emend. Nouioui et al. 2018

strain designation: N I 9100, UNCC 27 B

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
43424no
69480no91.667
69480100positive

colony morphology

@refcolony colormedium used
69355Light ivory (1015)suter without tyrosine
69355Light ivory (1015), brown beige (1011)ISP 4
69355Light ivory (1015), pale brown (8025)suter with tyrosine
69355Ochre brown (8001), copper brown (8004)ISP 2
69355Ochre brown (8001), copper brown (8004)ISP 3
69355Pale brown (8025)ISP 5
69355Pale brown (8025)ISP 7
69355Sand yellow (1002)ISP 6

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69355yesAerial myceliumSignal white (9003)ISP 2
69355yesAerial myceliumTraffic white (9016), platinum grey (7036)ISP 3
69355yesAerial myceliumSignal white (9003)ISP 4
69355yesAerial myceliumGrey white (9002)ISP 5
69355noAerial myceliumISP 6
69355yesAerial myceliumTelegrey 4 (7047)ISP 7
69355noAerial myceliumsuter with tyrosine
69355yesAerial myceliumPale brown (8025)suter without tyrosine

pigmentation

@refproductionname
69355noMelanin
69355nosoluble pigment

multimedia

@refmultimedia contentintellectual property rightscaption
10692https://www.dsmz.de/microorganisms/photos/DSM_43021-1.jpg© Leibniz-Institut DSMZ
10692https://www.dsmz.de/microorganisms/photos/DSM_43021.jpg© Leibniz-Institut DSMZMedium 65 28°C
66793EM_DSM_43021_1.jpg© HZI/Manfred Rohdeelectron microscopic image
69355DSM_43021_image3.jpegHelmholtz-Zentrum für Infektionsforschung GmbHPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)
69355DSM_43021_image4.jpegHelmholtz-Zentrum für Infektionsforschung GmbHPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10692ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
10692GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
10692STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
10692positivegrowth28mesophilic
18737positiveoptimum30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 43424
  • oxygen tolerance: aerobe

spore formation

@refspore descriptionspore formationconfidence
18737Formation of sporangiayes
43424yes
69481yes100
69480yes100

compound production

  • @ref: 18737
  • compound: Angucycline antibiotic WS 79089B

halophily

@refsaltgrowthtested relationconcentration
43424NaClpositivegrowth2.5 %
69355NaClpositivegrowth0 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4), MK-9(H2), MK-9(H6), MK-9(III,VIII-H4), MK-9(H2), MK-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4342422599arabinose+assimilation
4342462968cellulose-assimilation
4342416947citrate+assimilation
4342428757fructose+assimilation
4342417234glucose+assimilation
4342417268myo-inositol-assimilation
4342437684mannose-assimilation
4342417632nitrate+reduction
4342416634raffinose+assimilation
4342426546rhamnose-assimilation
4342428017starch+hydrolysis
4342417992sucrose+assimilation
4342418222xylose+assimilation
6935522599arabinose+growth
6935562968cellulose-growth
6935528757fructose+growth
6935517234glucose+growth
6935517268inositol+growth
6935537684mannose-growth
6935516634raffinose-growth
6935526546rhamnose+growth
6935517992sucrose-growth
6935518222xylose+growth
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

@refChebi-IDmetaboliteproduction
4342415688acetoinyes
4342416136hydrogen sulfideno
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
4342415688acetoin+
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
43424arginine dihydrolase+3.5.3.6
43424lysine decarboxylase+/-4.1.1.18
43424ornithine decarboxylase+/-4.1.1.17
43424gelatinase-
43424urease-3.5.1.5
43424tryptophan deaminase-4.1.99.1
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase-3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382alpha-mannosidase-3.2.1.24

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69355---+-++---+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69355++-+/-++-+/-+/-+/-+/--+-++/-+/--+/-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18737-++++----+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
10692vegetable garden soil
67770Garden soilChapel Hill, NCUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Garden
#Environmental#Terrestrial#Soil
#Host#Plants
#Host Body-Site#Plant#Fruit (Seed)

taxonmaps

  • @ref: 69479
  • File name: preview.99_2084.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_239;96_1144;97_1343;98_1627;99_2084&stattab=map
  • Last taxonomy: Streptosporangium
  • 16S sequence: X89947
  • Sequence Identity:
  • Total samples: 1306
  • soil counts: 1189
  • aquatic counts: 39
  • animal counts: 36
  • plant counts: 42

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
106921Risk group (German classification)
187371

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptosporangium roseum 16S rRNA geneU489961413ena479432
20218S.roseum gene for 16S ribosomal RNAX704251366ena479432
10692S.roseum DNA for 16S ribosomal RNA gene (strain DSM43021T)X899471450ena479432

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptosporangium roseum DSM 43021GCA_000024865completencbi479432
66792Streptosporangium roseum DSM 43021479432.6completepatric479432
66792Streptosporangium roseum DSM 43021479432.19plasmidpatric479432
66792Streptosporangium roseum NI 9100, DSM 43021646311958completeimg479432

GC content

@refGC-contentmethod
6777070.3thermal denaturation, midpoint method (Tm)
6777070.6
6777070.86genome sequence analysis
6777070high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno91.336yes
flagellatedno98.066yes
gram-positiveyes88.309no
anaerobicno99.14yes
aerobicyes92.574yes
halophileno91.944yes
spore-formingyes93.692no
thermophileno98.953yes
glucose-utilyes89.519no
glucose-fermentno89.846no

External links

@ref: 10692

culture collection no.: DSM 43021, ATCC 12428, CBS 313.56, IAM 14294, IFO 3776, JCM 3005, KCC A-0005, NBRC 3776, NCIB 10171, NRRL B-2505, RIA 470, BCRC 12463, BCRC 13667, CGMCC 4.1208, CGMCC 4.2071, IMSNU 20328, KCTC 9067, MTCC 332, NCIMB 10171, NRRL 2505, VKM Ac-807

straininfo link

  • @ref: 85616
  • straininfo: 35579

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology2775496Purification and N-terminal amino-acid sequences of bacterial malate dehydrogenases from six actinomycetales strains and from Phenylobacterium immobile, strain E.Rommel TO, Hund HK, Speth AR, Lingens FBiol Chem Hoppe Seyler10.1515/bchm3.1989.370.2.7631989Actinomycetales/*enzymology, Amino Acid Sequence, Ammonium Sulfate, Chromatography, Affinity, Chromatography, DEAE-Cellulose, Chromatography, Ion Exchange, Culture Media, Electrophoresis, Polyacrylamide Gel, Gram-Negative Bacteria/*enzymology, Malate Dehydrogenase/*analysis/isolation & purification, Molecular Sequence DataCultivation
Metabolism24389065Identification and characterization of 2-oxoglutarate-dependent dioxygenases catalyzing selective cis-hydroxylation of proline and pipecolinic acid from actinomycetes.Hara R, Uchiumi N, Kino KJ Biotechnol10.1016/j.jbiotec.2013.12.0032013Actinobacteria/classification/*enzymology, Bacterial Proteins/genetics/physiology, Carboxylic Acids/*metabolism, Dioxygenases/genetics/*physiology, Hydroxylation, Hydroxyproline/*metabolism, Ketoglutaric Acids/*metabolism, Models, ChemicalEnzymology
Phylogeny26507967Analysis of recombinase A (recA/RecA) in the actinobacterial family Streptosporangiaceae and identification of molecular signatures.Meyers PRSyst Appl Microbiol10.1016/j.syapm.2015.10.0012015Actinobacteria/*enzymology/genetics, Cluster Analysis, DNA Gyrase/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Genetic Variation, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/*genetics, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino AcidGenetics
Phylogeny26767659Streptosporangium lutulentum sp. nov., Streptosporangium fenghuangense sp. nov. and Streptosporangium corydalis sp. nov., three novel actinobacterial species isolated from National Forest Park of Fenghuang Mountain.Fang B, Liu H, Pan T, Liu C, Guan X, He H, Yan K, Li J, Xiang W, Wang XAntonie Van Leeuwenhoek10.1007/s10482-016-0650-42016Actinobacteria/*classification/genetics/isolation & purification/ultrastructure, Bacterial Typing Techniques, Base Composition, *Environment, *Environmental Microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Metabolism26814669Chemical diversity of labdane-type bicyclic diterpene biosynthesis in Actinomycetales microorganisms.Yamada Y, Komatsu M, Ikeda HJ Antibiot (Tokyo)10.1038/ja.2015.1472016Actinomycetales/*enzymology/genetics, Bacterial Proteins/chemistry/genetics/metabolism, Cell Engineering, Cyclization, Databases, Chemical, Diterpenes/chemistry/*metabolism, Streptomyces/geneticsEnzymology
Enzymology27157442Cultivation and purification of two stereoselective imine reductases from Streptosporangium roseum and Paenibacillus elgii.Lenz M, Scheller PN, Richter SM, Hauer B, Nestl BMProtein Expr Purif10.1016/j.pep.2016.05.0032016Actinobacteria/enzymology/*genetics, *Bacterial Proteins/biosynthesis/chemistry/genetics/isolation & purification, Escherichia coli/chemistry/genetics/*metabolism, *Oxidoreductases/biosynthesis/chemistry/genetics/isolation & purification, Paenibacillus/enzymology/*genetics, Recombinant Proteins/biosynthesis/chemistry/genetics/isolation & purificationPhylogeny
Enzymology27464826The biochemical characterization of three imine-reducing enzymes from Streptosporangium roseum DSM43021, Streptomyces turgidiscabies and Paenibacillus elgii.Scheller PN, Nestl BMAppl Microbiol Biotechnol10.1007/s00253-016-7740-02016Actinobacteria/*enzymology, Biotransformation, Enzyme Inhibitors/metabolism, Enzyme Stability, Hydrogen-Ion Concentration, Imines/*metabolism, Oxidation-Reduction, Oxidoreductases/chemistry/isolation & purification/*metabolism, Paenibacillus/*enzymology, Solvents/metabolism, Streptomyces/*enzymology, Substrate Specificity, TemperatureMetabolism
Metabolism35076224Combining Precursor-Directed Engineering with Modular Designing: An Effective Strategy for De Novo Biosynthesis of l-DOPA in Bacillus licheniformis.Xu Y, Li Y, Wu Z, Lu Y, Tao G, Zhang L, Ding Z, Shi GACS Synth Biol10.1021/acssynbio.1c004112022*Bacillus licheniformis/metabolism, Bioreactors, Fermentation, Levodopa/metabolism, Metabolic Engineering, Tyrosine/metabolismBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10692Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43021)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43021
18737Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43021.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
43424Matt Nolan, Johannes Sikorski, Marlen Jando, Susan Lucas, Alla Lapidus, Tijana Glavina Del Rio, Feng Chen, Hope Tice, Sam Pitluck, Jan-Fang Cheng, Olga Chertkov, David Sims, Linda Meincke, Thomas Brettin, Cliff Han, John C. Detter, David Bruce, Lynne Goodwin, Miriam Land, Loren Hauser, Yun-Juan Chang, Cynthia D. Jeffries, Natalia Ivanova, Konstantinos Mavromatis, Natalia Mikhailova, Amy Chen, Krishna Palaniappan, Patrick Chain, Manfred Rohde, Markus Göker, Jim Bristow, Jonathan A. Eisen, Victor Markowitz, Philip Hugenholtz, Nikos C. Kyrpides, Hans-Peter Klenk10.4056/sigs.631049Complete genome sequence of Streptosporangium roseum type strain (NI 9100)Stand Genomic Sci 2: 29-37 201021304675
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69355Wink, J.https://cdn.dsmz.de/wink/DSM%2043021.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85616Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID35579.1StrainInfo: A central database for resolving microbial strain identifiers