Strain identifier
BacDive ID: 16539
Type strain:
Species: Nonomuraea pusilla
Strain Designation: A-118, A3
Strain history: KCC A-0144 <-- H. Nonomura A-118.
NCBI tax ID(s): 46177 (species)
General
@ref: 10977
BacDive-ID: 16539
DSM-Number: 43357
keywords: genome sequence, 16S sequence, Bacteria, mesophilic
description: Nonomuraea pusilla A-118 is a mesophilic bacterium that was isolated from soil.
NCBI tax id
- NCBI tax id: 46177
- Matching level: species
strain history
doi: 10.13145/bacdive16539.20221219.7.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Streptosporangiales
- family: Streptosporangiaceae
- genus: Nonomuraea
- species: Nonomuraea pusilla
- full scientific name: Nonomuraea pusilla corrig. (Nonomura and Ohara 1971) Zhang et al. 1998
synonyms
@ref synonym 20215 Actinomadura pusilla 20215 Nonomuria pusilla 20215 Microtetraspora pusilla
@ref: 10977
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptosporangiaceae
genus: Nonomuraea
species: Nonomuraea pusilla
full scientific name: Nonomuraea pusilla (Nonomura and Ohara 1971) Zhang et al. 1998 emend. Nouioui et al. 2018
strain designation: A-118, A3
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 95.929
multimedia
@ref | multimedia content | caption | intellectual property rights |
---|---|---|---|
10977 | https://www.dsmz.de/microorganisms/photos/DSM_43357.jpg | Medium 65 37°C | © Leibniz-Institut DSMZ |
66793 | EM_DSM_43357_1.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_43357_2.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_43357_3.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_43357_4.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_43357_5.jpg | electron microscopic image | © HZI/Manfred Rohde |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
10977 | CZAPEK PEPTONE AGAR (DSMZ Medium 83) | yes | https://mediadive.dsmz.de/medium/83 | Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water |
37279 | MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum | yes | Distilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g) | |
10977 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
37279 | positive | growth | 37 | mesophilic |
10977 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
- @ref: 69480
- spore formation: yes
- confidence: 97.506
compound production
@ref | compound |
---|---|
10977 | actitiocin |
20216 | Actinotiocin |
halophily
- @ref: 69480
- halophily level: non-halophilic
- confidence: 95.848
observation
- @ref: 67770
- observation: quinones: MK-9(H4), MK-9(H6), MK-9(H4), MK-9(H2)
metabolite production
- @ref: 67770
- metabolite: actinotiocin
- production: yes
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
10977 | soil | ||||
67770 | Soil, dry-heated | Yamanashi Pref. | Japan | JPN | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_6928.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_239;96_3254;97_3993;98_5096;99_6928&stattab=map
- Last taxonomy: Nonomuraea pusilla subclade
- 16S sequence: U48978
- Sequence Identity:
- Total samples: 1377
- soil counts: 1122
- aquatic counts: 31
- animal counts: 43
- plant counts: 181
Safety information
risk assessment
- @ref: 10977
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Microtetraspora pusilla strain ATCC27296 16S ribosomal RNA gene, partial sequence | AF051384 | 77 | ena | 46177 |
20218 | Microtetraspora pusilla strain ATCC27296 16S ribosomal RNA gene, partial sequence | AF051391 | 193 | ena | 46177 |
20218 | Nonomuraea pusilla gene for 16S ribosomal RNA, partial sequence | D85491 | 1471 | ena | 46177 |
20218 | Microtetraspora pusilla 16S rRNA gene | U48978 | 1414 | ena | 46177 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Nonomuraea pusilla strain DSM 43357 | 46177.4 | wgs | patric | 46177 |
66792 | Nonomuraea pusilla DSM 43357 | 2599185218 | draft | img | 46177 |
67770 | Nonomuraea pusilla DSM 43357 | GCA_900109355 | scaffold | ncbi | 46177 |
66792 | Streptococcus pneumoniae SMRU2570 | GCA_001142865 | scaffold | patric | 1313 |
GC content
@ref | GC-content | method |
---|---|---|
67770 | 72.4 | genome sequence analysis |
67770 | 68 | thermal denaturation, midpoint method (Tm) |
67770 | 68.3 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 10977
culture collection no.: DSM 43357, ATCC 27296, CBS 262.72, IFO 14684, KCC A-0144, NBRC 14684, NCIMB 11116, JCM 3144, BCRC 11619, CECT 3284, CIP 106954, IMET 9586, IMSNU 20095, KCTC 9278, MTCC 1121, NBIMCC 1641, NRRL B-16126, VKM Ac-1508
straininfo link
@ref | passport |
---|---|
20218 | http://www.straininfo.net/strains/153492 |
20218 | http://www.straininfo.net/strains/105148 |
20218 | http://www.straininfo.net/strains/230652 |
20218 | http://www.straininfo.net/strains/153493 |
20218 | http://www.straininfo.net/strains/168236 |
20218 | http://www.straininfo.net/strains/333464 |
20218 | http://www.straininfo.net/strains/153497 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
10977 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43357) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-43357 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20216 | Curators of the HKI | http://www.leibniz-hki.de/de/ | Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
37279 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/19141 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66793 | Mukherjee et al. | 10.1038/nbt.3886 | GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life | 35: 676-683 2017 | 28604660 | |
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) |