Strain identifier

BacDive ID: 16503

Type strain: Yes

Species: Streptomyces endophyticus

Strain history: <- S.-K. Tang, Yunnan Univ., Kunming; YIM 65594 <- J. Li, Yunnan Univ., Kunming

NCBI tax ID(s): 714166 (species)

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General

@ref: 16372

BacDive-ID: 16503

DSM-Number: 41984

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Streptomyces endophyticus DSM 41984 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from surface-sterilized roots of Artemisia annua L..

NCBI tax id

  • NCBI tax id: 714166
  • Matching level: species

strain history

  • @ref: 16372
  • history: <- S.-K. Tang, Yunnan Univ., Kunming; YIM 65594 <- J. Li, Yunnan Univ., Kunming

doi: 10.13145/bacdive16503.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces endophyticus
  • full scientific name: Streptomyces endophyticus Li et al. 2013

@ref: 16372

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces endophyticus

full scientific name: Streptomyces endophyticus Li et al. 2013

type strain: yes

Morphology

cell morphology

  • @ref: 30422
  • gram stain: positive
  • cell length: 0.775 µm
  • cell width: 0.42 µm
  • motility: no

colony morphology

@refcolony colormedium used
69316Beige (1001), Light ivory (1015)ISP 3
69316Beige (1001), Light ivory (1015)ISP 4
69316Beige (1001), Light ivory (1015)suter with tyrosine
69316Beige (1001), Beige brown (8024)ISP 2
69316Light ivory (1015), Beige (1001)suter without tyrosine
69316Light ivory (1015), Brown beige (1011)ISP 5
69316Light ivory (1015), Ochre yellow (1024)ISP 7
69316Sand yellow (1002)ISP 6

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69316yesAerial myceliumOyster white (1013)ISP 2
69316noAerial myceliumISP 3
69316noAerial myceliumISP 4
69316noAerial myceliumISP 5
69316noAerial myceliumISP 6
69316yesAerial myceliumSignal white (9003)ISP 7
69316noAerial myceliumsuter with tyrosine
69316noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
30422no
69316yesMelanin
69316yessoluble pigmentOchre yellow (1024)

multimedia

@refmultimedia contentcaptionintellectual property rights
16372https://www.dsmz.de/microorganisms/photos/DSM_41984.jpgMedium 65 28°C© Leibniz-Institut DSMZ
69316DSM_41984_image3.jpegPlates (84, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69316DSM_41984_image4.jpegPlates (84, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16372STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf
16372GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
16372ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16372positivegrowth28mesophilic
30422positivegrowth10-37
30422positiveoptimum24psychrophilic

culture pH

@refabilitytypepHPH range
30422positivegrowth06-09alkaliphile
30422positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 30422
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30422
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
30422NaClpositivegrowth0-10 %
30422NaClpositiveoptimum5 %
69316NaClpositivegrowth0-5 %

observation

  • @ref: 30422
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6931622599arabinose+growth
3042216449alanine+carbon source
3042222599arabinose+carbon source
3042229016arginine+carbon source
3042222653asparagine+carbon source
3042217057cellobiose+carbon source
3042228757fructose+carbon source
3042228260galactose+carbon source
3042217754glycerol+carbon source
3042215428glycine+carbon source
30422182404-hydroxy-L-proline+carbon source
3042217716lactose+carbon source
3042217306maltose+carbon source
3042237684mannose+carbon source
3042228044phenylalanine+carbon source
3042226546rhamnose+carbon source
3042233942ribose+carbon source
3042217822serine+carbon source
3042253424tween 20+carbon source
3042253423tween 40+carbon source
3042253426tween 80+carbon source
6931662968cellulose+growth
6931628757fructose+growth
6931617234glucose+growth
6931617268inositol+growth
6931637684mannose+growth
6931616634raffinose+growth
6931626546rhamnose+growth
6931617992sucrose+growth
6931618222xylose+growth
6837917632nitrate+reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
30422catalase+1.11.1.6
30422gelatinase+
30422urease+3.5.1.5
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382leucine arylamidase+3.4.11.1
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69316+-++-+++-++--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69316+++/-+/-++/-+/---++-+-+++--

Isolation, sampling and environmental information

isolation

  • @ref: 16372
  • sample type: surface-sterilized roots of Artemisia annua L.
  • host species: Artemisia annua
  • geographic location: Yunnan province, Kunming
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root (Rhizome)
#Host Body-Site#Plant#Sterilized plant part

Safety information

risk assessment

  • @ref: 16372
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
16372Streptomyces endophyticus strain YIM 65594 16S ribosomal RNA gene, partial sequenceGU3671541522ena714166
16372Streptomyces endophyticus strain CS3KG2LA133 16S ribosomal RNA gene, partial sequenceDSM_419841439ena714166

GC content

  • @ref: 16372
  • GC-content: 72

External links

@ref: 16372

culture collection no.: DSM 41984, CCTCC AA 209036, YIM 65594

straininfo link

  • @ref: 85536
  • straininfo: 406229

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22389285Streptomyces endophyticus sp. nov., an endophytic actinomycete isolated from Artemisia annua L.Li J, Zhao GZ, Zhu WY, Huang HY, Xu LH, Zhang S, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.035725-02012Artemisia annua/*microbiology, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/analysisGenetics
Phylogeny25663029Streptomyces fractus sp. nov., a novel streptomycete isolated from the gut of a South African termite.Rohland J, Meyers PRAntonie Van Leeuwenhoek10.1007/s10482-015-0404-82015Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Gastrointestinal Tract/microbiology, Isoptera/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, South Africa, Streptomyces/*classification/genetics/*isolation & purificationGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16372Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41984)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41984
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30422Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2675828776041
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69316Wink, J.https://cdn.dsmz.de/wink/DSM%2041984.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
85536Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID406229.1StrainInfo: A central database for resolving microbial strain identifiers