Strain identifier

BacDive ID: 16495

Type strain: Yes

Species: Streptomyces staurosporininus

Strain Designation: BK179

Strain history: KACC 20912 <-- B.-Y. Kim et al. BK179.

NCBI tax ID(s): 887469 (species)

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General

@ref: 18076

BacDive-ID: 16495

DSM-Number: 42057

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, pigmented

description: Streptomyces staurosporininus BK179 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and has a Sand yellow , ivory pigmentation.

NCBI tax id

  • NCBI tax id: 887469
  • Matching level: species

strain history

@refhistory
18076<- J. Sweezy, NRRL; NRRL B-24850
67770KACC 20912 <-- B.-Y. Kim et al. BK179.

doi: 10.13145/bacdive16495.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces staurosporininus
  • full scientific name: Streptomyces staurosporininus Kim et al. 2012

@ref: 18076

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces staurosporininus

full scientific name: Streptomyces staurosporininus Kim et al. 2012

strain designation: BK179

type strain: yes

Morphology

cell morphology

  • @ref: 30259
  • gram stain: positive

colony morphology

@refcolony colormedium used
69333Beige (1001)ISP 6
69333Ivory (1014)suter without tyrosine
69333Light ivory (1015)ISP 4
69333Sand yellow (1002)suter with tyrosine

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69333yesAerial myceliumPure white (9010)ISP 2
69333yesAerial myceliumSignal white (9003)ISP 3
69333yesAerial myceliumTraffic white (9016)ISP 4
69333yesAerial myceliumPure white (9010), signal grey (7004)ISP 5
69333noAerial myceliumISP 6
69333yesAerial myceliumSignal white (9003), grey white (9002)ISP 7
69333noAerial myceliumsuter with tyrosine
69333noAerial myceliumsuter without tyrosine

pigmentation

  • @ref: 69333
  • production: yes
  • color: Sand yellow (1002), ivory (1014)
  • name: soluble pigment

multimedia

@refmultimedia contentcaptionintellectual property rights
18076https://www.dsmz.de/microorganisms/photos/DSM_42057.jpgMedium 252 28°C© Leibniz-Institut DSMZ
69333DSM_42057_image3.jpegPlates (84, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69333DSM_42057_image4.jpegPlates (84, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18076ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
18076STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
18076GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
18076positivegrowth28mesophilic
30259positivegrowth10-30
67770positivegrowth28mesophilic

culture pH

  • @ref: 30259
  • ability: positive
  • type: growth
  • pH: 04-10
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 30259
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30259
  • spore formation: yes

compound production

  • @ref: 18076
  • compound: staurosporine

halophily

@refsaltgrowthtested relationconcentration
30259NaClpositivegrowth5 %
69333NaClpositivegrowth0-2.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3025918403L-arabitol+carbon source
3025917057cellobiose+carbon source
3025917306maltose+carbon source
3025929864mannitol+carbon source
3025928053melibiose+carbon source
3025917268myo-inositol+carbon source
3025916634raffinose+carbon source
302594853esculin+hydrolysis
6933322599arabinose-growth
6933362968cellulose+/-growth
6933328757fructose+/-growth
6933317234glucose+growth
6933317268inositol+growth
6933337684mannose+/-growth
6933316634raffinose+growth
6933326546rhamnose-growth
6933317992sucrose-growth
6933318222xylose+growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 67770
  • Chebi-ID: 15738
  • metabolite: staurosporine
  • production: yes

enzymes

@refvalueactivityec
30259urease+3.5.1.5
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69333--++-+/-+/-+-++--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69333++/-+-++/-+/-+/-+++++-+++++/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
18076hay meadow soilNorthumberland, Cockle Park Experimental FarmUnited KingdomGBREurope
67770Hay meadow soil at Cockle Park Experimental FarmNorthumberlandUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Meadow
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

  • @ref: 18076
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18076
  • description: Streptomyces staurosporininus partial 16S rRNA gene, strain BK179
  • accession: FR692111
  • length: 1457
  • database: ena
  • NCBI tax ID: 887469

GC content

@refGC-contentmethod
1807671.2thermal denaturation, midpoint method (Tm)
3025971.2

External links

@ref: 18076

culture collection no.: DSM 42057, KACC 20912, NRRL B-24850, JCM 19087

straininfo link

  • @ref: 85528
  • straininfo: 401051

literature

  • topic: Phylogeny
  • Pubmed-ID: 21669925
  • title: Streptomyces staurosporininus sp. nov., a staurosporine-producing actinomycete.
  • authors: Kim BY, Zucchi TD, Fiedler HP, Goodfellow M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.031922-0
  • year: 2011
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Staurosporine/*biosynthesis, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
18076Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42057)https://www.dsmz.de/collection/catalogue/details/culture/DSM-42057
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30259Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2660028776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69333Wink, J.https://cdn.dsmz.de/wink/DSM%2042057.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
85528Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID401051.1StrainInfo: A central database for resolving microbial strain identifiers