Strain identifier
BacDive ID: 16477
Type strain: 
Species: Streptomyces caeruleatus
Strain Designation: GIMN4.002
Strain history: <- NRRL; NRRL B-24802
NCBI tax ID(s): 661399 (species)
version 8.1 (current version)
General
@ref: 17965
BacDive-ID: 16477
DSM-Number: 42045
keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive
description: Streptomyces caeruleatus GIMN4.002 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from tomato rhizosphere soil.
NCBI tax id
- NCBI tax id: 661399
- Matching level: species
strain history
- @ref: 17965
- history: <- NRRL; NRRL B-24802
doi: 10.13145/bacdive16477.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces caeruleatus
- full scientific name: Streptomyces caeruleatus Zhu et al. 2011
@ref: 17965
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces caeruleatus
full scientific name: Streptomyces caeruleatus Zhu et al. 2011
strain designation: GIMN4.002
type strain: yes
Morphology
cell morphology
- @ref: 29630
- gram stain: positive
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 18734 | Black brown (8022) | 10-14 days | ISP 2 |
| 18734 | Wine red (3005) | 10-14 days | ISP 3 |
| 18734 | Purple violet (4007) | 10-14 days | ISP 4 |
| 18734 | Wine red (3005) | 10-14 days | ISP 5 |
| 18734 | Yellow grey (7034) | 10-14 days | ISP 6 |
| 18734 | Black brown (8022) | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | complex name | complex color | medium name |
|---|---|---|---|---|
| 18734 | yes | Aerial Mycelium | Signal grey (7004) | ISP 2 |
| 18734 | yes | Aerial Mycelium | Cream (9001) | ISP 3 |
| 18734 | yes | Aerial Mycelium | Signal grey (7004) | ISP 4 |
| 18734 | no | ISP 5 | ||
| 18734 | yes | Aerial Mycelium | Cream (9001) | ISP 6 |
| 18734 | yes | Aerial Mycelium | Black grey (7021) | ISP 7 |
pigmentation
- @ref: 29630
- production: yes
multimedia
- @ref: 17965
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_42045.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 17965 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 18734 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 18734 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 18734 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 18734 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 18734 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 18734 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 17965 | ISP2 MEDIUM (DSMZ Medium 987) | yes | https://mediadive.dsmz.de/medium/987 | Name: ISP 2 MEDIUM (DSMZ Medium 987) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 17965 | positive | growth | 28 | mesophilic |
| 18734 | positive | optimum | 28 | mesophilic |
| 29630 | positive | growth | 28 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 29630
- oxygen tolerance: aerobe
spore formation
- @ref: 29630
- spore formation: yes
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 29630 | 22599 | arabinose | + | carbon source |
| 29630 | 29864 | mannitol | + | carbon source |
| 29630 | 26546 | rhamnose | + | carbon source |
| 29630 | 18222 | xylose | + | carbon source |
metabolite production
- @ref: 29630
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: yes
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 29630 | catalase | + | 1.11.1.6 |
| 68382 | alkaline phosphatase | + | 3.1.3.1 |
| 68382 | esterase (C 4) | - | |
| 68382 | esterase lipase (C 8) | + | |
| 68382 | lipase (C 14) | + | |
| 68382 | leucine arylamidase | + | 3.4.11.1 |
| 68382 | valine arylamidase | + | |
| 68382 | cystine arylamidase | + | 3.4.11.3 |
| 68382 | trypsin | + | 3.4.21.4 |
| 68382 | alpha-chymotrypsin | + | 3.4.21.1 |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | |
| 68382 | alpha-galactosidase | + | 3.2.1.22 |
| 68382 | beta-galactosidase | + | 3.2.1.23 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | alpha-glucosidase | + | 3.2.1.20 |
| 68382 | beta-glucosidase | + | 3.2.1.21 |
| 68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68382 | alpha-mannosidase | + | 3.2.1.24 |
| 68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
| @ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 18734 | + | - | + | + | + | + | + | + | + | + | + | + | + | - | + | + | + | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 17965
- sample type: tomato rhizosphere soil
- geographic location: Guangzhou
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Soil |
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Rhizosphere |
taxonmaps
- @ref: 69479
- File name: preview.99_826.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_109;98_118;99_826&stattab=map
- Last taxonomy: Streptomyces
- 16S sequence: GQ329712
- Sequence Identity:
- Total samples: 872
- soil counts: 433
- aquatic counts: 18
- animal counts: 71
- plant counts: 350
Safety information
risk assessment
- @ref: 17965
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17965
- description: Streptomyces caeruleatus strain GIMN4.002 16S ribosomal RNA gene, partial sequence
- accession: GQ329712
- length: 1397
- database: ena
- NCBI tax ID: 661399
GC content
- @ref: 17965
- GC-content: 70.7
External links
@ref: 17965
culture collection no.: DSM 42045, CCTCC M 208213, NRRL B-24802
straininfo link
- @ref: 85512
- straininfo: 404070
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 20363928 | Streptomyces caeruleatus sp. nov., with dark blue diffusible pigment. | Zhu HH, Guo J, Yao Q, Yang SZ, Deng MR, Li TH | Int J Syst Evol Microbiol | 10.1099/ijs.0.017392-0 | 2010 | *Antibiosis, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Lycopersicon esculentum, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Pigments, Biological/*metabolism, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification/physiology | Genetics |
| Phylogeny | 21930680 | Streptomyces shaanxiensis sp. nov., a blue pigment-producing streptomycete from sewage irrigation soil. | Lin YB, Wang XY, Fang H, Ma YN, Tang J, Tang M, Wei GH | Int J Syst Evol Microbiol | 10.1099/ijs.0.029959-0 | 2011 | Bacterial Typing Techniques, DNA, Bacterial/genetics, Molecular Sequence Data, *Phylogeny, *Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage, *Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification | Genetics |
| Phylogeny | 31219415 | Streptomyces cyaneochromogenes sp. nov., a blue pigment-producing actinomycete from manganese-contaminated soil. | Tang X, Zhao J, Li K, Chen Z, Sun Y, Gao J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003406 | 2019 | Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Manganese, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, *Soil Pollutants, Streptomyces/classification, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
| Phylogeny | 34775568 | Streptomyces blattellae, a novel actinomycete isolated from the in vivo of a Blattella germanica. | Liu GM, Yuan LL, Zhang LL, Zeng H | Antonie Van Leeuwenhoek | 10.1007/s10482-021-01681-w | 2021 | *Actinobacteria/genetics, DNA, Bacterial/genetics, Humans, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Streptomyces/genetics | Pathogenicity |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 17965 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42045) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-42045 | ||||
| 18734 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM42045.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 29630 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26020 | 28776041 | ||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 68382 | Automatically annotated from API zym | ||||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 85512 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID404070.1 | StrainInfo: A central database for resolving microbial strain identifiers |