Strain identifier
BacDive ID: 16466
Type strain: 
Species: Streptomyces artemisiae
Strain history: W.-J. Li YIM 63135.
NCBI tax ID(s): 481002 (species)
version 9 (current version)
General
@ref: 16033
BacDive-ID: 16466
DSM-Number: 41953
keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive
description: Streptomyces artemisiae DSM 41953 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from surface-sterilized tissue of Artemisia annua L..
NCBI tax id
- NCBI tax id: 481002
- Matching level: species
strain history
| @ref | history |
|---|---|
| 16033 | <- S.-K. Tang, YIM; YIM 63135 <- G.-Z. Zhao |
| 67770 | W.-J. Li YIM 63135. |
doi: 10.13145/bacdive16466.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces artemisiae
- full scientific name: Streptomyces artemisiae Zhao et al. 2010
@ref: 16033
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces artemisiae
full scientific name: Streptomyces artemisiae Zhao et al. 2010
type strain: yes
Morphology
cell morphology
- @ref: 29381
- gram stain: positive
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 19832 | Pastel yellow | 10-14 days | ISP 2 |
| 19832 | Brown beige | 10-14 days | ISP 3 |
| 19832 | Brown beige | 10-14 days | ISP 4 |
| 19832 | Beige | 10-14 days | ISP 5 |
| 19832 | Sand yellow | 10-14 days | ISP 6 |
| 19832 | Beige | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | medium name |
|---|---|---|
| 19832 | no | ISP 2 |
| 19832 | no | ISP 3 |
| 19832 | no | ISP 4 |
| 19832 | no | ISP 5 |
| 19832 | no | ISP 6 |
| 19832 | no | ISP 7 |
pigmentation
- @ref: 29381
- production: yes
multimedia
- @ref: 16033
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_41953.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 16033 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 19832 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 19832 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 19832 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 19832 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 19832 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 19832 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 16033 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://mediadive.dsmz.de/medium/84 | Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 16033 | positive | growth | 28 |
| 19832 | positive | optimum | 28 |
| 29381 | positive | growth | 10-45 |
| 29381 | positive | optimum | 28 |
| 67770 | positive | growth | 28 |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 29381 | positive | growth | 05-09 | alkaliphile |
| 29381 | positive | optimum | 7.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 29381
- oxygen tolerance: aerobe
spore formation
- @ref: 29381
- spore formation: yes
halophily
- @ref: 29381
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: <5 %
observation
- @ref: 67770
- observation: quinones: MK-9(H6), MK-9(H8)
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 19832 | 17234 | glucose | + | |
| 19832 | 22599 | arabinose | + | |
| 19832 | 17992 | sucrose | + | |
| 19832 | 18222 | xylose | - | |
| 19832 | 17268 | myo-inositol | + | |
| 19832 | 29864 | mannitol | + | |
| 19832 | 28757 | fructose | + | |
| 19832 | 26546 | rhamnose | + | |
| 19832 | 16634 | raffinose | + | |
| 19832 | 62968 | cellulose | - | |
| 29381 | 28260 | galactose | + | carbon source |
| 29381 | 17234 | glucose | + | carbon source |
| 29381 | 17754 | glycerol | + | carbon source |
| 29381 | 37684 | mannose | + | carbon source |
| 29381 | 16634 | raffinose | + | carbon source |
| 29381 | 26546 | rhamnose | + | carbon source |
| 29381 | 30911 | sorbitol | + | carbon source |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 29381 | catalase | + | 1.11.1.6 |
| 68382 | alkaline phosphatase | + | 3.1.3.1 |
| 68382 | esterase (C 4) | + | |
| 68382 | esterase lipase (C 8) | + | |
| 68382 | lipase (C 14) | - | |
| 68382 | leucine arylamidase | + | 3.4.11.1 |
| 68382 | valine arylamidase | + | |
| 68382 | cystine arylamidase | + | 3.4.11.3 |
| 68382 | trypsin | - | 3.4.21.4 |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | |
| 68382 | alpha-galactosidase | - | 3.2.1.22 |
| 68382 | beta-galactosidase | + | 3.2.1.23 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | alpha-glucosidase | + | 3.2.1.20 |
| 68382 | beta-glucosidase | - | 3.2.1.21 |
| 68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68382 | alpha-mannosidase | + | 3.2.1.24 |
| 68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
| @ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 19832 | + | + | + | - | + | + | + | - | - | + | + | - | + | - | + | - | + | + | - |
Isolation, sampling and environmental information
isolation
| @ref | sample type | host species | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|---|
| 16033 | surface-sterilized tissue of Artemisia annua L. | Artemisia annua | Yunnan Province | China | CHN | Asia |
| 67770 | Surface-sterilized tissue of Artemisia annua L. | Artemisia annua | Yunnan Province, south-west China | China | CHN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Sterilized plant part |
taxonmaps
- @ref: 69479
- File name: preview.99_37807.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_9745;97_11742;98_27782;99_37807&stattab=map
- Last taxonomy: Streptomyces
- 16S sequence: EU200685
- Sequence Identity:
- Total samples: 119
- soil counts: 49
- aquatic counts: 5
- animal counts: 63
- plant counts: 2
Safety information
risk assessment
- @ref: 16033
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16033
- description: Streptomyces artemisiae strain YIM 63135 16S ribosomal RNA gene, partial sequence
- accession: EU200685
- length: 1419
- database: nuccore
- NCBI tax ID: 481002
GC content
| @ref | GC-content | method |
|---|---|---|
| 16033 | 72.6 | high performance liquid chromatography (HPLC) |
| 29381 | 72.6 |
External links
@ref: 16033
culture collection no.: DSM 41953, CCTCC AA 208059, YIM 63135, JCM 19150
straininfo link
- @ref: 85502
- straininfo: 397954
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 19648351 | Streptomyces artemisiae sp. nov., isolated from surface-sterilized tissue of Artemisia annua L. | Zhao GZ, Li J, Qin S, Huang HY, Zhu WY, Xu LH, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.011965-0 | 2009 | Artemisia annua/*microbiology, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Streptomyces/*classification/genetics/*isolation & purification/metabolism | Metabolism |
| Phylogeny | 21724951 | Streptomyces pharmamarensis sp. nov. isolated from a marine sediment. | Carro L, Zuniga P, de la Calle F, Trujillo ME | Int J Syst Evol Microbiol | 10.1099/ijs.0.034066-0 | 2011 | Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Geologic Sediments/*microbiology, Mediterranean Sea, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, Pigments, Biological/metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Spores, Bacterial/cytology, Streptomyces/*classification/genetics/*isolation & purification/physiology, Vitamin K 2/analysis | Genetics |
| Phylogeny | 22527624 | Streptomyces phytohabitans sp. nov., a novel endophytic actinomycete isolated from medicinal plant Curcuma phaeocaulis. | Bian GK, Qin S, Yuan B, Zhang YJ, Xing K, Ju XY, Li WJ, Jiang JH | Antonie Van Leeuwenhoek | 10.1007/s10482-012-9737-8 | 2012 | China, Curcuma/*microbiology/physiology, Endophytes/classification/genetics/*isolation & purification/physiology, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, Plants, Medicinal/*microbiology/physiology, Streptomyces/classification/genetics/*isolation & purification/physiology | Enzymology |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 16033 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41953) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-41953 | |||
| 19832 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM41953.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 29381 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25789 | 28776041 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 68382 | Automatically annotated from API zym | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 85502 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID397954.1 | StrainInfo: A central database for resolving microbial strain identifiers |