Strain identifier

BacDive ID: 16463

Type strain: Yes

Species: Streptomyces marokkonensis

Strain Designation: AP1, R-22003

Strain history: LMG 23016 <-- B. Bouizgarne Ap1 (=R-22003).

NCBI tax ID(s): 324855 (species)

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General

@ref: 10450

BacDive-ID: 16463

DSM-Number: 41918

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Streptomyces marokkonensis AP1 is an aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from rhizosphere soil of Argania spinosa L..

NCBI tax id

  • NCBI tax id: 324855
  • Matching level: species

strain history

@refhistory
10450<- Y. Ouhdouch, Univ. Cadi Ayyad, Marrakech, Morocco; AP1 <- B. Bouizgarne et al.
67770LMG 23016 <-- B. Bouizgarne Ap1 (=R-22003).

doi: 10.13145/bacdive16463.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces marokkonensis
  • full scientific name: Streptomyces marokkonensis Bouizgarne et al. 2009

@ref: 10450

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces marokkonensis

full scientific name: Streptomyces marokkonensis Bouizgarne et al. 2009

strain designation: AP1, R-22003

type strain: yes

Morphology

cell morphology

  • @ref: 29350
  • gram stain: positive
  • motility: no

colony morphology

@refcolony colormedium used
69310Green Brown (8000), Olive brown (8008)ISP 2
69310Green brown (8000), Terra brown (8028)ISP 5
69310Concrete grey (7023), Terra brown (8028)ISP 4
69310Brown green (6008), Olive grey (7002)ISP 7
69310Ivory (1014), Grey beige (1019)ISP 3
69310Olive brown (8008)suter with tyrosine
69310Olive brown (8008)suter without tyrosine
69310Pale brown (8025), Brown beige (1011)ISP 6

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69310yesAerial myceliumSilk grey (7044)ISP 2
69310yesAerial myceliumGrey white (9002)ISP 3
69310yesAerial myceliumOyster white (1013)ISP 4
69310yesAerial myceliumSilk grey (7044)ISP 5
69310noAerial myceliumISP 6
69310yesAerial myceliumStone grey (7030), agate grey (7038)ISP 7
69310yesAerial myceliumStone grey (7030), telegrey 4 (7047)suter with tyrosine
69310yesAerial myceliumStone grey (7030), telegrey 4 (7047)suter without tyrosine

pigmentation

@refproductionnamecolor
29350yes
69310noMelanin
69310yessoluble pigmentOchre yellow (1042), sand yellow (1002), green brown (8000), ochre yellow (1024)

multimedia

@refmultimedia contentcaptionintellectual property rights
10450https://www.dsmz.de/microorganisms/photos/DSM_41918.jpgMedium 65 28°C© Leibniz-Institut DSMZ
69310DSM_41918_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69310DSM_41918_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

  • @ref: 10450
  • name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/65
  • composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
10450positivegrowth28mesophilic
18730positiveoptimum28mesophilic
29350positivegrowth15-40
29350positiveoptimum29mesophilic
67770positivegrowth28mesophilic

culture pH

  • @ref: 29350
  • ability: positive
  • type: growth
  • pH: 4.5-10
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 29350
  • oxygen tolerance: aerobe

halophily

  • @ref: 69310
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-7.5 %

observation

  • @ref: 29350
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2935022599arabinose+carbon source
2935017057cellobiose+carbon source
2935028757fructose+carbon source
2935028260galactose+carbon source
2935017234glucose+carbon source
2935017754glycerol+carbon source
2935017716lactose+carbon source
2935017306maltose+carbon source
2935029864mannitol+carbon source
2935037684mannose+carbon source
2935026546rhamnose+carbon source
2935017814salicin+carbon source
2935017992sucrose+carbon source
2935018222xylose+carbon source
2935017632nitrate+reduction
6931022599arabinose+/-growth
6931062968cellulose+growth
6931028757fructose+growth
6931017234glucose+growth
6931017268inositol+/-growth
6931037684mannose+growth
6931016634raffinose-growth
6931026546rhamnose+growth
6931017992sucrose+/-growth
6931018222xylose+growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 67770
  • metabolite: chaninin
  • production: yes

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69310---+------+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69310++++/-++/----++-+-++++-

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinent
10450rhizosphere soil of Argania spinosa L.Argania spinosaMoroccoMARAfrica
67770Rhizosphere soil of the argan tree (Argania spinosa L.) in the south of MoroccoArgania spinosa L.

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants#Tree
#Host Body-Site#Plant#Rhizosphere

Safety information

risk assessment

  • @ref: 10450
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 10450
  • description: Streptomyces marokkonensis 16S rRNA gene
  • accession: AJ965470
  • length: 1479
  • database: ena
  • NCBI tax ID: 324855

GC content

@refGC-contentmethod
1045070high performance liquid chromatography (HPLC)
1045060-78
2935070

External links

@ref: 10450

culture collection no.: DSM 41918, LMG 23016, CCMM 2001, JCM 17027

straininfo link

  • @ref: 85499
  • straininfo: 137805

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19628602Streptomyces marokkonensis sp. nov., isolated from rhizosphere soil of Argania spinosa L.Bouizgarne B, Lanoot B, Loqman S, Sproer C, Klenk HP, Swings J, Ouhdouch YInt J Syst Evol Microbiol10.1099/ijs.0.011387-02009DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Phylogeny, Plant Roots/microbiology, RNA, Ribosomal, 16S/genetics, Sapotaceae/*microbiology, *Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purificationGenetics
Phylogeny19643894Streptomyces thinghirensis sp. nov., isolated from rhizosphere soil of Vitis vinifera.Loqman S, Bouizgarne B, Barka EA, Clement C, von Jan M, Sproer C, Klenk HP, Ouhdouch YInt J Syst Evol Microbiol10.1099/ijs.0.008946-02009DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification, *VitisGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
10450Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41918)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41918
18730Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41918.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29350Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2576228776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69310Wink, J.https://cdn.dsmz.de/wink/DSM%2041918.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
85499Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID137805.1StrainInfo: A central database for resolving microbial strain identifiers