Strain identifier

BacDive ID: 164621

Type strain: Yes

Species: Motilibacter deserti

Strain Designation: E257

Strain history: C.-H. Sun; Chin. Acad. of Med. Sci., China; E257.

NCBI tax ID(s): 2714956 (species)

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General

@ref: 67770

BacDive-ID: 164621

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, colony-forming

description: Motilibacter deserti E257 is an obligate aerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from Desert soil in Cholistan Desert.

NCBI tax id

  • NCBI tax id: 2714956
  • Matching level: species

strain history

  • @ref: 67770
  • history: C.-H. Sun; Chin. Acad. of Med. Sci., China; E257.

doi: 10.13145/bacdive164621.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Motilibacterales
  • family: Motilibacteraceae
  • genus: Motilibacter
  • species: Motilibacter deserti
  • full scientific name: Motilibacter deserti Liu et al. 2021

@ref: 67770

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Frankiales

family: Motilibacteraceae

genus: Motilibacter

species: Motilibacter deserti

full scientific name: Motilibacter deserti Liu et al. 2021

strain designation: E257

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell lengthcell widthcell shapemotility
69480positive98.97
69640positive2.1-3.0 µm1.0-1.4 µmrod-shapedyes

colony morphology

  • @ref: 69640
  • colony color: orange
  • colony shape: circular
  • incubation period: 4 days
  • medium used: ISP 2 agar

Culture and growth conditions

culture medium

@refnamegrowth
69640ISP 2yes
69640Trypticase Soy Agar (TSA)yes
69640ISP 3yes
69640ISP 4yes
69640ISP 5yes
69640Nutrient agar (NA)yes
69640ISP 7no
69640R2A MEDIUM (DSMZ Medium 830)yes
69640ISP 2 agaryes

culture temp

@refgrowthtypetemperaturerange
67770positivegrowth37mesophilic
69640positivegrowth20-40
69640positiveoptimum37mesophilic

culture pH

@refabilitytypepH
69640positivegrowth6-9
69640positiveoptimum7-8

Physiology and metabolism

oxygen tolerance

  • @ref: 69640
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidencetype of spore
69480no93.072
69640nospore

halophily

@refsaltgrowthtested relationconcentration
69640NaClpositivegrowth0 %(w/v)
69640NaClpositiveoptimum0 %(w/v)

observation

@refobservation
67770quinones: MK-9(H4) and MK-9(H2)
69640mycelium-forming

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6964017128adipate-assimilation
6964030849L-arabinose-assimilation
69640506227N-acetyl-D-glucosamine-assimilation
6964032032potassium gluconate-assimilation
6964018305arbutin-builds acid from
6964017108D-arabinose-builds acid from
6964012936D-galactose-builds acid from
6964017634D-glucose-builds acid from
6964017113erythritol-builds acid from
6964016813galactitol-builds acid from
6964062345L-rhamnose-builds acid from
6964017716lactose-builds acid from
69640320061methyl alpha-D-glucopyranoside-builds acid from
6964074863methyl beta-D-xylopyranoside-builds acid from
6964017814salicin-builds acid from
6964017992sucrose-builds acid from
6964017234glucose-fermentation
6964016024D-mannose+assimilation
6964017306maltose+assimilation
6964053258sodium citrate+assimilation
6964017057cellobiose+builds acid from
6964028847D-fucose+builds acid from
6964062318D-lyxose+builds acid from
6964016024D-mannose+builds acid from
6964016988D-ribose+builds acid from
6964016443D-tagatose+builds acid from
6964065327D-xylose+builds acid from
6964028087glycogen+builds acid from
6964030849L-arabinose+builds acid from
6964018287L-fucose+builds acid from
6964065328L-xylose+builds acid from
6964017306maltose+builds acid from
6964029864mannitol+builds acid from
6964028053melibiose+builds acid from
6964028017starch+builds acid from
6964032528turanose+builds acid from
696404853esculin+hydrolysis
696405291gelatin+hydrolysis
6964028017starch+hydrolysis
6964053424tween 20+hydrolysis
6964053423tween 40+hydrolysis
6964053426tween 80+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6964016136hydrogen sulfideno
6964035581indoleno

enzymes

@refvalueactivityec
69640N-acetyl-beta-glucosaminidase+3.2.1.52
69640acid phosphatase+/-3.1.3.2
69640alkaline phosphatase-3.1.3.1
69640esterase (C 4)+
69640esterase Lipase (C 8)+
69640alpha-galactosidase-3.2.1.22
69640beta-galactosidase-3.2.1.23
69640beta-glucosidase-3.2.1.21
69640alpha-mannosidase-3.2.1.24
69640valine arylamidase-
69640catalase+1.11.1.6
69640cytochrome oxidase-1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic locationlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperature
67770Desert soil in Cholistan DesertPakistanPAKAsia
69640Desert soilPakistanPAKAsia2018-10-25Cholistan Desert28.606771.54Yeast-Malt Extract Agar4-8 weeks30

Sequence information

16S sequences

  • @ref: 67770
  • description: Motilibacter sp. strain E257 16S ribosomal RNA gene, partial sequence
  • accession: MT233298
  • length: 1428
  • database: ena
  • NCBI tax ID: 2714956

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
67770Motilibacter deserti E257GCA_011250635scaffoldncbi2714956
69640Whole genome shotgunJAANNP000000000ncbi2714956

GC content

  • @ref: 67770
  • GC-content: 73.6
  • method: genome sequence analysis

Genome-based predictions

predictions

  • trait: spore-forming
  • prediction: yes
  • confidence: 86
  • training_data: no

External links

@ref: 67770

culture collection no.: JCM 33651, CGMCC 1.17159

literature

  • topic: Phylogeny
  • Pubmed-ID: 33099259
  • title: Motilibacter deserti sp. nov. and Motilibacter aurantiacus sp. nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter.
  • authors: Liu SW, Ye JJ, Lu QP, Cheema MT, Abbas M, Huang DL, Sajid I, Sun CH
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/j.syapm.2020.126150
  • year: 2020
  • mesh: Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Desert Climate, Fatty Acids/chemistry, Nucleic Acid Hybridization, Pakistan, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/url
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69640Shao-Wei Liu, Jing-Jing Ye, Qin-Pei Lu, Mohsin Tassawar Cheema, Muhammad Abbas, Da-Lin Huang, Imran Sajid, Cheng-Hang SunMotilibacter deserti sp. nov. and Motilibacter aurantiacus sp. nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter10.1016/j.syapm.2020.126150