Strain identifier

BacDive ID: 16371

Type strain: Yes

Species: Streptomyces europaeiscabiei

Strain history: CIP <- 2001, K. Bouchek, INRA, France: strain CFBP 4497

NCBI tax ID(s): 146819 (species)

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General

@ref: 10586

BacDive-ID: 16371

DSM-Number: 41802

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped, plant pathogen

description: Streptomyces europaeiscabiei DSM 41802 is an obligate aerobe, mesophilic, Gram-positive plant pathogen that was isolated from common scab lesions on potato.

NCBI tax id

  • NCBI tax id: 146819
  • Matching level: species

strain history

@refhistory
10586<- NCPPB
346582001, K. Bouchek, INRA, France strain
119143CIP <- 2001, K. Bouchek, INRA, France: strain CFBP 4497

doi: 10.13145/bacdive16371.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces europaeiscabiei
  • full scientific name: Streptomyces europaeiscabiei Bouchek-Mechiche et al. 2000

@ref: 10586

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces europaeiscabiei

full scientific name: Streptomyces europaeiscabiei Bouchek-Mechiche et al. 2000

type strain: yes

Morphology

cell morphology

  • @ref: 119143
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 119143
  • hemolysis ability: 1

multimedia

@refmultimedia contentintellectual property rightscaption
10586https://www.dsmz.de/microorganisms/photos/DSM_41802-1.jpg© Leibniz-Institut DSMZ
10586https://www.dsmz.de/microorganisms/photos/DSM_41802.jpg© Leibniz-Institut DSMZMedium 65 28°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10586GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
34658MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
10586ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
10586STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
119143CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
10586positivegrowth28mesophilic
34658positivegrowth30mesophilic
119143positivegrowth25-41
119143nogrowth10psychrophilic
119143nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119143
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
119143NaClpositivegrowth0 %
119143NaClnogrowth2 %
119143NaClnogrowth4 %
119143NaClnogrowth6 %
119143NaClnogrowth8 %
119143NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1191434853esculin+hydrolysis
119143606565hippurate-hydrolysis
11914317632nitrate-respiration

metabolite production

  • @ref: 119143
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11914315688acetoin-
11914317234glucose-

enzymes

@refvalueactivityec
119143oxidase-
119143beta-galactosidase-3.2.1.23
119143alcohol dehydrogenase-1.1.1.1
119143gelatinase+
119143amylase-
119143DNase-
119143caseinase-3.4.21.50
119143catalase+1.11.1.6
119143tween esterase-
119143gamma-glutamyltransferase+2.3.2.2
119143lecithinase-
119143lipase-
119143lysine decarboxylase-4.1.1.18
119143ornithine decarboxylase-4.1.1.17
119143phenylalanine ammonia-lyase-4.3.1.24
119143protease-
119143tryptophan deaminase-
119143urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119143-+++-+----++-----+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119143+++++-++++++++++-+++++-++-+----+++---+--+++-+----++-----++-++-+++++--++-------+++-----+---+--+-----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
10586common scab lesions on potatoFranceFRAEurope
119143Solanum tuberosumFranceFRAEurope

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Other#Wound

Safety information

risk assessment

@refpathogenicity plantbiosafety levelbiosafety level comment
10586yes1Risk group (German classification)
1191431Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces europaeiscabiei strain CFBP 4497 clone ITS11 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296984278ena146819
20218Streptomyces europaeiscabiei strain CFBP 4497 clone ITS2 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296985279ena146819
20218Streptomyces europaeiscabiei strain CFBP 4497 clone ITS21 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296986278ena146819
20218Streptomyces europaeiscabiei strain CFBP 4497 clone ITS24 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296987279ena146819
20218Streptomyces europaeiscabiei strain CFBP 4497 clone ITS29 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296988278ena146819
20218Streptomyces europaeiscabiei strain CFBP 4497 16S ribosomal RNA gene, partial sequenceFJ0074061441ena146819
10586Streptomyces sp. 16S rRNA gene, isolate CFBP4497AJ0074231492ena146819

GC content

  • @ref: 10586
  • GC-content: 71.3

External links

@ref: 10586

culture collection no.: DSM 41802, CFBP 4497, NCPPB 4039, CIP 107142, ICMP 13714, CIP 107062

straininfo link

  • @ref: 85425
  • straininfo: 87859

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10826791DNA relatedness among strains of Streptomyces pathogenic to potato in France: description of three new species, S. europaeiscabiei sp. nov. and S. stelliscabiei sp. nov. associated with common scab, and S. reticuliscabiei sp. nov. associated with netted scab.Bouchek-Mechiche K, Gardan L, Normand P, Jouan BInt J Syst Evol Microbiol10.1099/00207713-50-1-912000Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Genes, rRNA/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Plant Diseases/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Solanum tuberosum/*microbiology, Streptomyces/*classification/genetics/isolation & purification/physiologyGenetics
30699653First Report of Streptomyces europaeiscabiei Causing Common Scab on Potato in Finland.Hiltunen LH, Kelloniemi J, Valkonen JPTPlant Dis10.1094/PDIS-03-14-0278-PDN2014

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10586Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41802)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41802
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34658Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4545
68382Automatically annotated from API zym
85425Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID87859.1StrainInfo: A central database for resolving microbial strain identifiers
119143Curators of the CIPCollection of Institut Pasteur (CIP 107142)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107142