Strain identifier

BacDive ID: 16364

Type strain: Yes

Species: Streptomyces mexicanus

Strain Designation: BM-B-384, CH-M-1035, NNRL B-24196

Strain history: CIP <- 2003, M.E. Flores, UNAM, Mexico: strain CH-M-1035

NCBI tax ID(s): 1305627 (strain), 178566 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 10580

BacDive-ID: 16364

DSM-Number: 41796

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic

description: Streptomyces mexicanus BM-B-384 is an obligate aerobe, mesophilic bacterium that builds an aerial mycelium and was isolated from soil at a sugar-cane fields.

NCBI tax id

NCBI tax idMatching level
178566species
1305627strain

strain history

@refhistory
10580<- C. Huitrón, UNAM; CH-M-1035
67770DSM 41796 <-- C. Huitrón CH-M-1035.
120931CIP <- 2003, M.E. Flores, UNAM, Mexico: strain CH-M-1035

doi: 10.13145/bacdive16364.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces mexicanus
  • full scientific name: Streptomyces mexicanus Petrosyan et al. 2003

@ref: 10580

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces mexicanus

full scientific name: Streptomyces mexicanus Petrosyan et al. 2003

strain designation: BM-B-384, CH-M-1035, NNRL B-24196

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no91.626
6948099.606positive
120931nopositiverod-shaped

colony morphology

@refcolony colorincubation periodmedium used
18721Sand yellow (1002)10-14 daysISP 2
18721Beige (1001)10-14 daysISP 3
18721Brown beige (1011)10-14 daysISP 4
18721Ivory (1014)10-14 daysISP 5
18721Beige (1001)10-14 daysISP 6
18721Beige (1001)10-14 daysISP 7
120931

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18721yesAerial MyceliumPlatinum grey (7036)ISP 2
18721yesAerial MyceliumPlatinum grey (7036)ISP 3
18721yesAerial MyceliumPlatinum grey (7036)ISP 4
18721yesAerial MyceliumIvory (1014)ISP 5
18721noISP 6
18721yesAerial MyceliumIvory (1014)ISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10580GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18721ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18721ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18721ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18721ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18721ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18721ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33203MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
10580ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
10580STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
120931CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
10580positivegrowth37mesophilic
18721positiveoptimum28mesophilic
33203positivegrowth37mesophilic
67770positivegrowth37mesophilic
120931positivegrowth25-45
120931nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120931
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.977

compound production

@refcompound
10580xylanase
10580B xylosidase
67770beta-xylosidase

halophily

@refsaltgrowthtested relationconcentration
120931NaClpositivegrowth0-8 %
120931NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis
12093116947citrate-carbon source
1209314853esculin-hydrolysis
120931606565hippurate+hydrolysis
12093117632nitrate-reduction
12093116301nitrite-reduction
12093117632nitrate-respiration

antibiotic resistance

  • @ref: 120931
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideyes
12093135581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole-
12093115688acetoin+
12093117234glucose-

enzymes

@refvalueecactivity
67770Xylanase3.2.1.8
68368gelatinase+
68368tryptophan deaminase4.1.99.1-
68368urease3.5.1.5-
68368ornithine decarboxylase4.1.1.17+
68368lysine decarboxylase4.1.1.18+
68368arginine dihydrolase3.5.3.6+
68368beta-galactosidase3.2.1.23+
120931oxidase-
120931beta-galactosidase3.2.1.23+
120931alcohol dehydrogenase1.1.1.1-
120931gelatinase+
120931amylase+
120931DNase-
120931caseinase3.4.21.50-
120931catalase1.11.1.6+
120931tween esterase-
120931gamma-glutamyltransferase2.3.2.2+
120931lecithinase-
120931lipase-
120931lysine decarboxylase4.1.1.18-
120931ornithine decarboxylase4.1.1.17-
120931phenylalanine ammonia-lyase4.3.1.24-
120931tryptophan deaminase-
120931urease3.5.1.5-
68382alkaline phosphatase3.1.3.1+
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase3.4.11.1+
68382valine arylamidase+
68382trypsin3.4.21.4-
68382alpha-chymotrypsin3.4.21.1-
68382acid phosphatase3.1.3.2+
68382naphthol-AS-BI-phosphohydrolase+
68382beta-galactosidase3.2.1.23+
68382beta-glucuronidase3.2.1.31-
68382alpha-glucosidase3.2.1.20+
68382N-acetyl-beta-glucosaminidase3.2.1.52+
68382alpha-mannosidase3.2.1.24+
68382alpha-fucosidase3.2.1.51-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18721++++++---++++-++++-
120931+++-+++--++-+-+-++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18721++++++----+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120931+++++-+-++++++-++-++++----+----+-+---------++----++--+++++-++-+-+------+++++---++---+---++++-++-++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
10580soil at a sugar-cane fieldsMorelos, ZacatepecMexicoMEXNorth America
67770Soil at a sugar-cane fieldMorelosMexicoMEXNorth America
120931Environment, Soil samplesMexicoMEXNorth America1982

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
105801Risk group (German classification)
187211German classification
1209311Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
10580Streptomyces sp. CH-M-1035 16S ribosomal RNA gene, partial sequenceAF4411681450ena178566
67770Streptomyces mexicanus gene for 16S rRNA, partial sequence, strain: NBRC 100915AB2499661483ena178566

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces mexicanus JCM 12681GCA_001313875contigncbi1305627
66792Streptomyces mexicanus JCM 126811305627.3wgspatric1305627
66792Streptomyces mexicanus JCM 126812675903513draftimg1305627

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes86no
motileno89.465no
gram-positiveyes84.132no
anaerobicno96.655no
halophileno89.118no
spore-formingno62.553no
thermophileno94.352yes
glucose-utilyes87.34no
aerobicyes91.76no
flagellatedno97.876no
glucose-fermentno79.86no

External links

@ref: 10580

culture collection no.: DSM 41796, JCM 12681, NRRL B-24196, CIP 108132, NBRC 100915, NCIMB 14336

straininfo link

  • @ref: 85419
  • straininfo: 100976

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12656184Streptomyces mexicanus sp. nov., a xylanolytic micro-organism isolated from soil.Petrosyan P, Garcia-Varela M, Luz-Madrigal A, Huitron C, Flores MEInt J Syst Evol Microbiol10.1099/ijs.0.02251-02003Base Sequence, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification/metabolism, Xylans/metabolismGenetics
Phylogeny24994773Streptomyces pluripotens sp. nov., a bacteriocin-producing streptomycete that inhibits meticillin-resistant Staphylococcus aureus.Lee LH, Zainal N, Azman AS, Eng SK, Ab Mutalib NS, Yin WF, Chan KGInt J Syst Evol Microbiol10.1099/ijs.0.065045-02014*Antibiosis, Avicennia, Bacterial Typing Techniques, Bacteriocins/*biosynthesis, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Malaysia, Methicillin-Resistant Staphylococcus aureus, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification/*metabolism, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitle
10580Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41796)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41796
18721Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41796.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33203Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5721
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
85419Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100976.1StrainInfo: A central database for resolving microbial strain identifiers
120931Curators of the CIPCollection of Institut Pasteur (CIP 108132)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108132