Strain identifier

BacDive ID: 1631

Type strain: Yes

Species: Bacteroides faecis

Strain Designation: MAJ27

Strain history: <- JW Bae, Kyung Hee Univ., Seoul, Korea

NCBI tax ID(s): 1077285 (strain), 674529 (species)

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General

@ref: 17776

BacDive-ID: 1631

DSM-Number: 24798

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, Gram-negative, rod-shaped, human pathogen

description: Bacteroides faecis MAJ27 is an anaerobe, Gram-negative, rod-shaped human pathogen that was isolated from faeces of healthy human.

NCBI tax id

NCBI tax idMatching level
674529species
1077285strain

strain history

@refhistory
17776<- JCM <- J.-W. Bae; MAJ27
67770J.-W. Bae MAJ27.
67771<- JW Bae, Kyung Hee Univ., Seoul, Korea

doi: 10.13145/bacdive1631.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Bacteroidaceae
  • genus: Bacteroides
  • species: Bacteroides faecis
  • full scientific name: Bacteroides faecis Kim et al. 2010

@ref: 17776

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Bacteroidaceae

genus: Bacteroides

species: Bacteroides faecis

full scientific name: Bacteroides faecis Kim et al. 2010

strain designation: MAJ27

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
29749negative1.5-2 µm1 µmrod-shapedno
67771rod-shapedno
67771negative
69480negative94.198

colony morphology

@refincubation period
177761-2 days
624971 day

pigmentation

  • @ref: 29749
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 17776
  • name: CHOPPED MEAT MEDIUM (DSMZ Medium 78)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/78
  • composition: Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78; with strain-specific modifications) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) Yeast extract 5.0 g/l K2HPO4 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water

culture temp

@refgrowthtypetemperature
17776positivegrowth37
29749positivegrowth25-43
29749positiveoptimum37
62497positivegrowth37
67770positivegrowth37
67771positivegrowth37

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
17776anaerobe
29749anaerobe
62497anaerobe
67771anaerobe

spore formation

@refspore formationconfidence
29749no
67771no
69480no91.4

observation

  • @ref: 29749
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2974922599arabinose+carbon source
2974917057cellobiose+carbon source
2974917234glucose+carbon source
2974917716lactose+carbon source
2974917306maltose+carbon source
2974937684mannose+carbon source
2974916634raffinose+carbon source
2974926546rhamnose+carbon source
2974917992sucrose+carbon source
2974918222xylose+carbon source
297494853esculin+hydrolysis
6836727082trehalose+builds acid from
6836762345L-rhamnose+builds acid from
6836730911sorbitol-builds acid from
6836716634raffinose+builds acid from
6836716024D-mannose+builds acid from
6836717057cellobiose-builds acid from
6836717754glycerol-builds acid from
683674853esculin+hydrolysis
683675291gelatin-hydrolysis
6836730849L-arabinose+builds acid from
6836765327D-xylose+builds acid from
6836717814salicin-builds acid from
6836717306maltose+builds acid from
6836717992sucrose+builds acid from
6836717716lactose+builds acid from
6836717634D-glucose+builds acid from
6836716199urea-hydrolysis
6836727897tryptophan+energy source
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose+fermentation
6838029985L-glutamate+degradation
6838017632nitrate-reduction
6838027897tryptophan+energy source

metabolite production

@refChebi-IDmetaboliteproduction
2974935581indoleyes
6836735581indoleyes
6838035581indoleyes

metabolite tests

@refChebi-IDmetaboliteindole test
2974935581indole+
6838035581indole+
6836735581indole+

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase+
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase+4.1.99.1
68380alpha-fucosidase+3.2.1.51
68380glutamate decarboxylase+4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase+3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68367beta-glucosidase+3.2.1.21
68367gelatinase-
68367urease-3.5.1.5

API 20A

@refINDUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRE
17776+-++/-+++-++-+--++/-+-++

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
17776--++-+++-+++++-+-+-+-+--+--+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling date
17776faeces of healthy humanRepublic of KoreaKORAsia
62497Human faeces2009
67770Human feces
67771From faeces of healthy humanRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_5196.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_21;96_684;97_1763;98_3873;99_5196&stattab=map
  • Last taxonomy: Bacteroides
  • 16S sequence: GQ496624
  • Sequence Identity:
  • Total samples: 7978
  • soil counts: 20
  • aquatic counts: 257
  • animal counts: 7699
  • plant counts: 2

Safety information

risk assessment

  • @ref: 17776
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacteroides faecis gene for 16S ribosomal RNA, partial sequence, strain: JCM 16478AB5476401475nuccore1077285
17776Bacteroides sp. MAJ27 16S ribosomal RNA gene, partial sequenceGQ4966241458nuccore1077285

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacteroides faecis MAJ271077285.3wgspatric1077285
66792Bacteroides faecis MAJ271077285.5wgspatric1077285
66792Bacteroides faecis MAJ272548876788draftimg1077285
66792Bacteroides faecis DSM 247982693429916draftimg1077285
67771Bacteroides faecis MAJ27GCA_000226135contigncbi1077285
67771Bacteroides faecis MAJ27GCA_900106755scaffoldncbi1077285

GC content

@refGC-content
1777642.7
6777141.7-43.7

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingno94.198yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)yes83yes
69480spore-formingspore-formingAbility to form endo- or exosporesno91.4yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no92.85yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.553no
69480flagellatedmotile2+Ability to perform flagellated movementno87.5yes

External links

@ref: 17776

culture collection no.: CCUG 60914, DSM 24798, JCM 16478, KCTC 5823

straininfo link

@refstraininfo
71277404697
71278409613

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20008108Bacteroides faecis sp. nov., isolated from human faeces.Kim MS, Roh SW, Bae JWInt J Syst Evol Microbiol10.1099/ijs.0.020024-02009Adult, Bacteroides/*classification/genetics/*isolation & purification/metabolism, Base Composition, DNA, Bacterial/genetics, Fatty Acids/metabolism, Feces/*microbiology, Humans, Male, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism
Genetics22072652Draft genome sequence of Bacteroides faecis MAJ27T, a strain isolated from human feces.Kim MS, Whon TW, Roh SW, Shin NR, Bae JWJ Bacteriol10.1128/JB.06210-112011Bacteroides/*genetics/isolation & purification, Base Sequence, Feces/*microbiology, *Genome, Bacterial, Humans, Molecular Sequence DataPathogenicity
Phylogeny22544795Bacteroides reticulotermitis sp. nov., isolated from the gut of a subterranean termite (Reticulitermes speratus).Sakamoto M, Ohkuma MInt J Syst Evol Microbiol10.1099/ijs.0.040931-02012Animals, Bacterial Typing Techniques, Bacteroides/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Gastrointestinal Tract/*microbiology, Isoptera/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analysisGenetics
Phylogeny33881983Bacteroides luhongzhouii sp. nov. and Bacteroides zhangwenhongii sp. nov., isolated from human faeces.Ge Y, Zhang G, Yang J, Lai XH, Jin D, Lu S, Pu J, Huang Y, Luo X, Zheng H, Zhang X, Xu JInt J Syst Evol Microbiol10.1099/ijsem.0.0047722021Bacterial Typing Techniques, Bacteroides/*classification/isolation & purification, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Humans, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/chemistryPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
17776Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24798)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24798
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
29749Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126131
62497Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 60914)https://www.ccug.se/strain?id=60914
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68367Automatically annotated from API 20A
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71277Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID404697.1StrainInfo: A central database for resolving microbial strain identifiers
71278Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID409613.1StrainInfo: A central database for resolving microbial strain identifiers