Strain identifier

BacDive ID: 16204

Type strain: Yes

Species: Streptomyces coelicoflavus

Culture col. no.: DSM 41471, INA 9630, JCM 6918

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General

@ref: 10335

BacDive-ID: 16204

DSM-Number: 41471

keywords: Bacteria, 16S sequence, genome sequence, mesophilic

description: Streptomyces coelicoflavus DSM 41471 is a mesophilic bacterium that builds an aerial mycelium.

strain history: <- N.S. Agre, INA

doi: 10.13145/bacdive16204.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Streptomycetales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces coelicoflavus
  • full scientific name: Streptomyces coelicoflavus (ex Ryabova and Preobrazhenskaya 1957) Terekhova 1986

@ref: 10335

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces coelicoflavus

full scientific name: Streptomyces coelicoflavus (ex Ryabova and Preobrazhenskaya 1957) Terekhova 1986

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19511Sand yellow10-14 daysISP 2
19511Sand yellow10-14 daysISP 3
19511Sand yellow10-14 daysISP 4
19511Sand yellow10-14 daysISP 5
19511Sand yellow10-14 daysISP 6
19511Pastel yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium namecomplex namecomplex color
19511noISP 2
19511yesISP 3Aerial MyceliumWindow grey
19511yesISP 4Aerial MyceliumWindow grey
19511noISP 5
19511noISP 6
19511noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10335GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
19511ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19511ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19511ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19511ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19511ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19511ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
10335positivegrowth28mesophilic
19511positiveoptimum28mesophilic

Physiology and metabolism

halophily

  • @ref: 19511
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 7.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activity
1951162968cellulose-
1951116634raffinose+
1951126546rhamnose+
1951128757fructose+
1951129864mannitol+
1951117268myo-inositol+
1951118222xylose-
1951117992sucrose-
1951122599arabinose+
1951117234glucose+/-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19511+-+------++-----+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19511------+--+-

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
103351Risk group (German classification)
195111Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces coelicoflavus strain JCM 6918 16S ribosomal RNA gene, partial sequenceAY9997521404ena285562
20218Streptomyces coelicoflavus gene for 16S rRNA, partial sequence, strain: NBRC 15399AB1846501480ena285562

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces coelicoflavus NBRC 15399GCA_003112555contigncbi285562
66792Streptomyces coelicoflavus F13GCA_011007525contigncbi285562
66792Streptomyces coelicoflavus SID14172GCA_010548435contigncbi285562
66792Streptomyces coelicoflavus SID14163GCA_010548385contigncbi285562
66792Streptomyces coelicoflavus strain NBRC 15399285562.5wgspatric285562

External links

@ref: 10335

culture collection no.: DSM 41471, INA 9630, JCM 6918

straininfo link

@refpassport
20218http://www.straininfo.net/strains/167084
20218http://www.straininfo.net/strains/167085
20218http://www.straininfo.net/strains/167086

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10335Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41471)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41471
19511Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41471.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)