Strain identifier
BacDive ID: 16184
Type strain: 
Species: Streptomyces macrosporus
Strain Designation: A1201
Strain history: J. Lacey A 1201 <-- INMI 2892.
NCBI tax ID(s): 44032 (species)
version 9 (current version)
General
@ref: 10313
BacDive-ID: 16184
DSM-Number: 41449
keywords: 16S sequence, Bacteria, spore-forming
description: Streptomyces macrosporus A1201 is a spore-forming bacterium that builds an aerial mycelium and was isolated from sewage compost and soil.
NCBI tax id
- NCBI tax id: 44032
- Matching level: species
strain history
| @ref | history |
|---|---|
| 10313 | <- J. Lacey, A1201 <- INMI |
| 67770 | J. Lacey A 1201 <-- INMI 2892. |
doi: 10.13145/bacdive16184.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces macrosporus
- full scientific name: Streptomyces macrosporus (ex Krassilnikov et al. 1968) Goodfellow et al. 1988
@ref: 10313
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces macrosporus
full scientific name: Streptomyces macrosporus (ex Krassilnikov et al. 1968) Goodfellow et al. 1988
strain designation: A1201
type strain: yes
Morphology
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 19499 | Yellow brown | 10-14 days | ISP 2 |
| 19499 | Yellow brown | 10-14 days | ISP 3 |
| 19499 | Yellow brown | 10-14 days | ISP 4 |
| 19499 | 10-14 days | ISP 5 | |
| 19499 | Yellow brown | 10-14 days | ISP 6 |
| 19499 | Red brown | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | medium name | complex name | complex color |
|---|---|---|---|---|
| 19499 | no | ISP 2 | ||
| 19499 | no | ISP 3 | ||
| 19499 | no | ISP 4 | ||
| 19499 | yes | ISP 5 | Aerial Mycelium | White |
| 19499 | no | ISP 6 | ||
| 19499 | yes | ISP 7 | Aerial Mycelium | White |
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 10313 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 10313 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
| 19499 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 19499 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 19499 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 19499 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 19499 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 19499 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 10313 | positive | growth | 45 |
| 19499 | positive | optimum | 45 |
| 67770 | positive | growth | 40 |
Physiology and metabolism
spore formation
- @ref: 19499
- spore description: Formation of spore chains (rectiflixibilis), spore surface smooth
- type of spore: spore
- spore formation: yes
halophily
- @ref: 19499
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0 %
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 19499 | 62968 | cellulose | - | |
| 19499 | 16634 | raffinose | - | |
| 19499 | 26546 | rhamnose | + | |
| 19499 | 28757 | fructose | + | |
| 19499 | 29864 | mannitol | - | |
| 19499 | 17268 | myo-inositol | + | |
| 19499 | 18222 | xylose | - | |
| 19499 | 17992 | sucrose | - | |
| 19499 | 22599 | arabinose | + | |
| 19499 | 17234 | glucose | + | |
| 68368 | 5291 | gelatin | + | hydrolysis |
| 68368 | 27897 | tryptophan | - | energy source |
| 68368 | 16199 | urea | + | hydrolysis |
| 68368 | 16947 | citrate | + | assimilation |
| 68368 | 18257 | ornithine | + | degradation |
| 68368 | 25094 | lysine | + | degradation |
| 68368 | 29016 | arginine | + | hydrolysis |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 68368 | 15688 | acetoin | no |
| 68368 | 35581 | indole | no |
| 68368 | 16136 | hydrogen sulfide | no |
metabolite tests
| @ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
|---|---|---|---|---|
| 68368 | 15688 | acetoin | - | |
| 68368 | 35581 | indole | - |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68368 | gelatinase | + | |
| 68368 | tryptophan deaminase | + | 4.1.99.1 |
| 68368 | urease | + | 3.5.1.5 |
| 68368 | ornithine decarboxylase | + | 4.1.1.17 |
| 68368 | lysine decarboxylase | + | 4.1.1.18 |
| 68368 | arginine dihydrolase | + | 3.5.3.6 |
| 68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
| @ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 19499 | + | + | + | + | + | - | + | + | - | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 10313
- sample type: sewage compost and soil
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Biodegradation | #Composting |
| #Engineered | #Waste | #Wastewater |
| #Environmental | #Terrestrial | #Soil |
taxonmaps
- @ref: 69479
- File name: preview.99_83776.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_2143;97_2583;98_58130;99_83776&stattab=map
- Last taxonomy: Streptomyces macrosporus subclade
- 16S sequence: Z68099
- Sequence Identity:
- Total samples: 7
- soil counts: 6
- plant counts: 1
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 10313 | 1 | Risk group (German classification) |
| 19499 | 1 | Risk group (German classification) |
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | S.macrosporus 16S rRNA gene | Z68099 | 1484 | nuccore | 44032 |
| 20218 | Streptomyces macrosporus gene for 16S rRNA, partial sequence, strain: NBRC 14748 | AB184616 | 1465 | nuccore | 44032 |
External links
@ref: 10313
culture collection no.: DSM 41449, ATCC 51533, IFO 14748, INMI 2892, JCM 6305, NBRC 14748, CGMCC 4.1994, HUT 6608, NCIMB 12473, VKM Ac-777
straininfo link
- @ref: 85247
- straininfo: 46156
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 17978221 | Streptomyces radiopugnans sp. nov., a radiation-resistant actinomycete isolated from radiation-polluted soil in China. | Mao J, Tang Q, Zhang Z, Wang W, Wei D, Huang Y, Liu Z, Shi Y, Goodfellow M | Int J Syst Evol Microbiol | 10.1099/ijs.0.65027-0 | 2007 | Bacterial Typing Techniques, China, Cobalt Radioisotopes, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, *Gamma Rays, Genes, rRNA, Molecular Sequence Data, Phenotype, RNA, Ribosomal, 16S/genetics, *Radiation Tolerance, Sequence Analysis, DNA, *Soil Microbiology, *Soil Pollutants, Radioactive, Species Specificity, Streptomyces/*classification/genetics/isolation & purification/physiology/*radiation effects | Genetics |
| Phylogeny | 24436069 | Streptomyces barkulensis sp. nov., isolated from an estuarine lake. | Ray L, Mishra SR, Panda AN, Rastogi G, Pattanaik AK, Adhya TK, Suar M, Raina V | Int J Syst Evol Microbiol | 10.1099/ijs.0.056614-0 | 2014 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Estuaries, Fatty Acids/chemistry, Geologic Sediments/microbiology, India, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
| Phylogeny | 29580317 | Streptomyces salilacus sp. nov., an actinomycete isolated from a salt lake. | Luo XX, Gao GB, Xia ZF, Chen ZJ, Wan CX, Zhang LL | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002703 | 2018 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Lakes/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 10313 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41449) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-41449 | |||
| 19499 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM41449.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 68368 | Automatically annotated from API 20E | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 85247 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID46156.1 | StrainInfo: A central database for resolving microbial strain identifiers |