Strain identifier

BacDive ID: 16184

Type strain: Yes

Species: Streptomyces macrosporus

Strain Designation: A1201

Strain history: J. Lacey A 1201 <-- INMI 2892.

NCBI tax ID(s): 44032 (species)

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General

@ref: 10313

BacDive-ID: 16184

DSM-Number: 41449

keywords: 16S sequence, Bacteria, spore-forming, thermophilic

description: Streptomyces macrosporus A1201 is a spore-forming, thermophilic bacterium that builds an aerial mycelium and was isolated from sewage compost and soil.

NCBI tax id

  • NCBI tax id: 44032
  • Matching level: species

strain history

@refhistory
10313<- J. Lacey, A1201 <- INMI
67770J. Lacey A 1201 <-- INMI 2892.

doi: 10.13145/bacdive16184.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces macrosporus
  • full scientific name: Streptomyces macrosporus (ex Krassilnikov et al. 1968) Goodfellow et al. 1988

@ref: 10313

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces macrosporus

full scientific name: Streptomyces macrosporus (ex Krassilnikov et al. 1968) Goodfellow et al. 1988

strain designation: A1201

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19499Yellow brown10-14 daysISP 2
19499Yellow brown10-14 daysISP 3
19499Yellow brown10-14 daysISP 4
1949910-14 daysISP 5
19499Yellow brown10-14 daysISP 6
19499Red brown10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium namecomplex namecomplex color
19499noISP 2
19499noISP 3
19499noISP 4
19499yesISP 5Aerial MyceliumWhite
19499noISP 6
19499yesISP 7Aerial MyceliumWhite

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10313GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
10313NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
19499ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19499ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19499ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19499ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19499ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19499ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
10313positivegrowth45thermophilic
19499positiveoptimum45thermophilic
67770positivegrowth40thermophilic

Physiology and metabolism

spore formation

  • @ref: 19499
  • spore description: Formation of spore chains (rectiflixibilis), spore surface smooth
  • type of spore: spore
  • spore formation: yes

halophily

  • @ref: 19499
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1949962968cellulose-
1949916634raffinose-
1949926546rhamnose+
1949928757fructose+
1949929864mannitol-
1949917268myo-inositol+
1949918222xylose-
1949917992sucrose-
1949922599arabinose+
1949917234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase+4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19499+++++-++--+

Isolation, sampling and environmental information

isolation

  • @ref: 10313
  • sample type: sewage compost and soil

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation#Composting
#Engineered#Waste#Wastewater
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_83776.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_2143;97_2583;98_58130;99_83776&stattab=map
  • Last taxonomy: Streptomyces macrosporus subclade
  • 16S sequence: Z68099
  • Sequence Identity:
  • Total samples: 7
  • soil counts: 6
  • plant counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
103131Risk group (German classification)
194991Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218S.macrosporus 16S rRNA geneZ680991484ena44032
20218Streptomyces macrosporus gene for 16S rRNA, partial sequence, strain: NBRC 14748AB1846161465ena44032

External links

@ref: 10313

culture collection no.: DSM 41449, ATCC 51533, IFO 14748, INMI 2892, JCM 6305, NBRC 14748, CGMCC 4.1994, HUT 6608, NCIMB 12473, VKM Ac-777

straininfo link

  • @ref: 85247
  • straininfo: 46156

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17978221Streptomyces radiopugnans sp. nov., a radiation-resistant actinomycete isolated from radiation-polluted soil in China.Mao J, Tang Q, Zhang Z, Wang W, Wei D, Huang Y, Liu Z, Shi Y, Goodfellow MInt J Syst Evol Microbiol10.1099/ijs.0.65027-02007Bacterial Typing Techniques, China, Cobalt Radioisotopes, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, *Gamma Rays, Genes, rRNA, Molecular Sequence Data, Phenotype, RNA, Ribosomal, 16S/genetics, *Radiation Tolerance, Sequence Analysis, DNA, *Soil Microbiology, *Soil Pollutants, Radioactive, Species Specificity, Streptomyces/*classification/genetics/isolation & purification/physiology/*radiation effectsGenetics
Phylogeny24436069Streptomyces barkulensis sp. nov., isolated from an estuarine lake.Ray L, Mishra SR, Panda AN, Rastogi G, Pattanaik AK, Adhya TK, Suar M, Raina VInt J Syst Evol Microbiol10.1099/ijs.0.056614-02014Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Estuaries, Fatty Acids/chemistry, Geologic Sediments/microbiology, India, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny29580317Streptomyces salilacus sp. nov., an actinomycete isolated from a salt lake.Luo XX, Gao GB, Xia ZF, Chen ZJ, Wan CX, Zhang LLInt J Syst Evol Microbiol10.1099/ijsem.0.0027032018Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Lakes/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10313Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41449)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41449
19499Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41449.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85247Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46156.1StrainInfo: A central database for resolving microbial strain identifiers