Strain identifier
BacDive ID: 16081
Type strain: 
Species: Streptomyces violaceoruber
Strain Designation: A3(2)
Strain history: KCC S-0979 <-- DSM 40783 <-- L. Ettlinger LGB A 3128 <-- G. Sermonti A 3(2) (one of the agar-decomposing strain (Waksman's 3443) from D. Erikson).
NCBI tax ID(s): 1935 (species)
version 9 (current version)
General
@ref: 9589
BacDive-ID: 16081
DSM-Number: 40783
keywords: 16S sequence, Bacteria
description: Streptomyces violaceoruber A3 is a bacterium that builds an aerial mycelium and was isolated from One of the agar-decomposing strain from D. Erikson.
NCBI tax id
- NCBI tax id: 1935
- Matching level: species
strain history
| @ref | history |
|---|---|
| 9589 | <- L. Ettlinger, LBG <- Sermonti (Streptomyces coelicolor A3(2)) |
| 67770 | KCC S-0979 <-- DSM 40783 <-- L. Ettlinger LGB A 3128 <-- G. Sermonti A 3(2) (one of the agar-decomposing strain (Waksman's 3443) from D. Erikson). |
doi: 10.13145/bacdive16081.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces violaceoruber
- full scientific name: Streptomyces violaceoruber (Waksman and Curtis 1916) Pridham 1970 (Approved Lists 1980)
synonyms
@ref synonym 20215 Actinopycnidium caeruleum 20215 Actinomyces tricolor 20215 Actinomyces anthocyanicus 20215 Actinomyces violaceolatus 20215 Streptomyces humiferus 20215 Streptomyces anthocyanicus 20215 Streptomyces tricolor 20215 Streptomyces violaceolatus 20215 Streptomyces roseodiastaticus 20215 Actinomyces violaceusruber 20215 Actinomyces coelescens 20215 Actinomyces roseodiastaticus 20215 Streptomyces coelescens
@ref: 9589
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces violaceoruber
full scientific name: Streptomyces violaceoruber (Waksman and Curtis 1916) Pridham 1970
strain designation: A3(2)
type strain: no
Morphology
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 19815 | Black grey | 10-14 days | ISP 2 |
| 19815 | Wine red | 10-14 days | ISP 3 |
| 19815 | Saphire blue | 10-14 days | ISP 4 |
| 19815 | Saphire blue | 10-14 days | ISP 5 |
| 19815 | Beige | 10-14 days | ISP 6 |
| 19815 | Brown beige | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | complex name | complex color | medium name |
|---|---|---|---|---|
| 19815 | yes | Aerial Mycelium | Iron grey (7011) | ISP 2 |
| 19815 | yes | Aerial Mycelium | Cream (9001) | ISP 3 |
| 19815 | yes | Aerial Mycelium | Iron grey (7011) | ISP 4 |
| 19815 | yes | Aerial Mycelium | Iron grey (7011) | ISP 5 |
| 19815 | no | ISP 6 | ||
| 19815 | yes | Aerial Mycelium | Cream (9001) | ISP 7 |
multimedia
- @ref: 9589
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40783.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 9589 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 19815 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 19815 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 19815 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 19815 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 19815 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 19815 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 9589 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf | |
| 9589 | STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 9589 | positive | growth | 28 |
| 19815 | positive | optimum | 28 |
| 67770 | positive | growth | 28 |
Physiology and metabolism
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 67770 | 2448 | actinorhodin | yes |
| 67770 | methylenomycin A | yes |
Isolation, sampling and environmental information
isolation
- @ref: 67770
- sample type: One of the agar-decomposing strain (Waksman's 3443) from D. Erikson
taxonmaps
- @ref: 69479
- File name: preview.99_357.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_91;97_97;98_106;99_357&stattab=map
- Last taxonomy: Streptomyces
- 16S sequence: AB184698
- Sequence Identity:
- Total samples: 5402
- soil counts: 2201
- aquatic counts: 991
- animal counts: 1970
- plant counts: 240
Safety information
risk assessment
- @ref: 9589
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Streptomyces violaceoruber strain DSM 40783 16S ribosomal RNA gene, partial sequence | GU383133 | 780 | nuccore | 1935 |
| 67770 | Streptomyces violaceoruber gene for 16S rRNA, partial sequence, strain: NBRC 15732 | AB184698 | 1467 | nuccore | 1935 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 67770 | 72 | thermal denaturation, midpoint method (Tm) |
| 67770 | 71.4 | Buoyant density centrifugation (BD) |
External links
@ref: 9589
culture collection no.: DSM 40783, JCM 4979, KCC S-0979, LBG A 3128, BCRC 12079, CECT 3080, IFO 15732, NBRC 15732
straininfo link
- @ref: 85150
- straininfo: 49796
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 9589 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40783) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40783 | |||
| 19815 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40783.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 85150 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49796.1 | StrainInfo: A central database for resolving microbial strain identifiers |