Strain identifier

BacDive ID: 160441

Type strain: Yes

Species: Microlunatus speluncae

Strain history: <- W.-J. Li, State Key Lab. for Biocontrol, Sun Yat-sen Univ., Guangzhou, People's Republic of China; SYSU K12189 <- M.-X. Han

NCBI tax ID(s): 2594267 (species)

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General

@ref: 67349

BacDive-ID: 160441

DSM-Number: 103947

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped

description: Microlunatus speluncae DSM 103947 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from karst cave.

NCBI tax id

  • NCBI tax id: 2594267
  • Matching level: species

strain history

  • @ref: 67349
  • history: <- W.-J. Li, State Key Lab. for Biocontrol, Sun Yat-sen Univ., Guangzhou, People's Republic of China; SYSU K12189 <- M.-X. Han

doi: 10.13145/bacdive160441.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Microlunatus
  • species: Microlunatus speluncae
  • full scientific name: Microlunatus speluncae Xie et al. 2020

@ref: 67349

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Propionibacteriales

family: Propionibacteriaceae

genus: Microlunatus

species: Microlunatus speluncae

full scientific name: Microlunatus speluncae Xie et al. 2020

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
68328positive1.5-2 µm0.7-0.9 µmrod-shapedno
69480no90.785
69480positive100

multicellular morphology

  • @ref: 68328
  • forms multicellular complex: yes
  • complex color: white substrate mycelia are produced on most media
  • medium name: ISP 2, 3, 4, 5 and Czapek's agar, Potato-glucose agar and nutrient agar media

pigmentation

  • @ref: 68328
  • production: no

Culture and growth conditions

culture medium

  • @ref: 67349
  • name: R2A MEDIUM (DSMZ Medium 830)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830
  • composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
67349positivegrowth28mesophilic
68328positivegrowth15-37

culture pH

  • @ref: 68328
  • ability: positive
  • type: growth
  • pH: 6-10
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 68328
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69480no98.806
69481yes100

halophily

  • @ref: 68328
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683285291gelatin-hydrolysis
6832828017starch-hydrolysis
68328milk-assimilation
6832862968cellulose-hydrolysis
6832853424tween 20-hydrolysis
6832853423tween 40-hydrolysis
6832853425tween 60-hydrolysis
6832853426tween 80-hydrolysis
6832817632nitrate-reduction
6832817057cellobiose+carbon source
6832812936D-galactose+carbon source
6832817634D-glucose+carbon source
6832817754glycerol+carbon source
6832817268myo-inositol+carbon source
6832817306maltose+carbon source
6832816899D-mannitol+carbon source
6832816551D-trehalose+carbon source
6832815824D-fructose-carbon source
6832816634raffinose-carbon source
6832816988D-ribose-carbon source
6832817317D-sorbose-carbon source
6832817924D-sorbitol-carbon source
6832817992sucrose-carbon source
6832817151xylitol-carbon source
6832865327D-xylose-carbon source
6832816643L-methionine+nitrogen source
6832815971L-histidine+nitrogen source
6832817368hypoxanthine+nitrogen source
6832817203L-proline+nitrogen source
6832817895L-tyrosine+nitrogen source
6832816467L-arginine-nitrogen source
6832816708adenine-nitrogen source
6832816414L-valine+nitrogen source
6832817561L-cysteine-nitrogen source
6832829985L-glutamate-nitrogen source
6832815428glycine-nitrogen source
6832817295L-phenylalanine-nitrogen source
6832817115L-serine-nitrogen source
6832816857L-threonine-nitrogen source
6832816828L-tryptophan-nitrogen source

metabolite production

  • @ref: 68328
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
68328urease-3.5.1.5
68328catalase-1.11.1.6
68328cytochrome oxidase-1.9.3.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    68328C16:01.2
    68328C18:00.6
    68328C14:0 iso10.3
    68328C15:0 iso23.3
    68328C16:0 iso19
    68328C15:0 anteiso35
    68328C17:0 anteiso0.6
    68328C15:1 w5c1.9
    68328C17:1 iso w5c2.9
    68328C16:1 2OH0.7
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A medium
  • incubation temperature: 28
  • incubation time: 7
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperature
67349karst caveXingyi (E:104° 46', N:25° 9')ChinaCHNAsia25.15104.767
68328soilKarst cave in Xingyi county, Guizhou provinceChinaCHNAsia25.15104.767humic acid-vitamin agar2 weeks28

Sequence information

16S sequences

  • @ref: 68328
  • description: Microlunatus speluncae 16S ribosomal RNA gene, partial sequence
  • accession: MF480393
  • length: 1515
  • database: ena
  • NCBI tax ID: 2594267

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Microlunatus speluncae strain SYSU K121892594267.3wgspatric2594267
68328Microlunatus speluncae SYSU K12189GCA_009299835contigncbi2594267

GC content

  • @ref: 68328
  • GC-content: 69.4
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno94.145yes
gram-positiveyes85.671no
anaerobicno99.278yes
aerobicyes91.036yes
halophileno91.022no
spore-formingno55.175no
glucose-utilyes77.616no
flagellatedno98.105yes
thermophileno99.068yes
glucose-fermentno84.164no

External links

@ref: 67349

culture collection no.: DSM 103947, KCTC 39847, SYSU K12189

straininfo link

  • @ref: 112688
  • straininfo: 402914

literature

  • topic: Phylogeny
  • Pubmed-ID: 31485839
  • title: Microlunatus speluncae sp. nov., a novel actinobacterium isolated from a Karstic subterranean environment sample.
  • authors: Xie YG, Fang BZ, Han MX, Liu L, Jiao JY, Zhang XT, Xiao M, Li WJ
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-019-01321-4
  • year: 2019
  • mesh: Actinomycetales/classification/genetics, Base Composition/genetics, Glycolipids/metabolism, Phosphatidylglycerols/metabolism, Phosphatidylinositols/metabolism, Phylogeny, Propionibacteriaceae/classification/*genetics, RNA, Ribosomal, 16S/genetics
  • topic2: Metabolism

Reference

@idauthorstitledoi/urlcataloguejournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67349Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103947Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103947)
68328Yuan-Guo Xie, Bao-Zhu Fang, Ming-Xian Han, Lan Liu, Jian-Yu Jiao, Xiao-Tong Zhang, Min Xiao & Wen-Jun LiMicrolunatus speluncae sp. nov., a novel actinobacterium isolated from a Karstic subterranean environment sample10.1007/s10482-019-01321-4Antonie Van Leeuwenhoek 113: 117-125 2020
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
112688Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402914.1